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Enzyme sensor

An enzyme sensor and enzyme layer technology, applied in the field of enzyme sensors, can solve problems such as poor reproducibility and stability, inability to continuously detect samples, troublesome installation, etc.

Inactive Publication Date: 2004-07-21
OCEAN UNIV OF CHINA
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

Obviously, frequent manual installation of the enzyme membrane is cumbersome, and the reproducibility and stability are poor, so it cannot be used for continuous detection of samples.
In addition, the existing enzyme sensors have low sensitivity and often cannot detect organophosphorus pesticides in seawater

Method used

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Embodiment Construction

[0008] Such as figure 1 , the present invention is made up of identification element 4 and is connected to the pH electrode 7 that is placed in measuring pool 6 as transducer via flexible pipe 5, is characterized in that identification element 4 is a tube that has tube hole or capillary C at both ends A structure composed of column 1 and immobilized enzyme 2 inside. That is, the recognition element 4 is made by injecting the immobilized enzyme 2 into the plastic or glass column 1, and is connected with other parts (measurement pool, injection valve) through thin tubes whose two ends are integral with the column, so it is very convenient to use. Usually, according to the concentration range of organophosphorus pesticides in the sample to be tested, immobilized enzymes 2 with different activity units are added to the column 1 to form a variety of enzyme columns that can test different concentrations of pesticides—recognition element 4 .

[0009] In order to reduce the volume an...

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Abstract

An enzyme transducer is composed of identification element and pH electrode being connected to energy converter and being placed in determination pool. The identification element is a structural body which is composed of tubular column and fixed enzyme contained in it as fixed enzyme is semisolid state of acetylcholinesterase using gel as a carrier. In addition, enzyme blocking layer is set at bottom of tubular column.

Description

technical field [0001] The invention relates to an enzyme sensor for detecting organophosphorus pesticides in water bodies. Background technique [0002] Enzyme sensors usually consist of two parts: a recognition element (immobilized enzyme) and a transducer (an electrode placed in a measurement cell). The existing recognition element is generally an enzyme membrane formed by immobilizing a certain biological enzyme on a carrier such as a cellulose membrane, and is in the form of a sheet. Before use, the operator needs to install the enzyme membrane on the sensitive part of the transducer (electrode) surface. Obviously, frequent manual installation of the enzyme membrane is cumbersome, and the reproducibility and stability are poor, so it cannot be used for continuous detection of samples. In addition, the existing enzyme sensors have low sensitivity and often cannot detect organophosphorus pesticides in seawater. Contents of the invention [0003] The purpose of the pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 孟范平朱小山何东海杨正先马冬李桂芳
Owner OCEAN UNIV OF CHINA
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