Chicken escherichia coli heterotype outer membrane protein cell engineered bacterial strain preparing method

Abstract

The invention is a producing method for a kind of project strain of chicken bacillus coli allotype tunica adventitia protein cell, at first, the ill materials are inoculated, incubated, and stores the separated bacterium; the second step selects out the dominant serotype strain tunica adventitia protein; the third step takes out the dominant serotype tunica adventitia protein and finds out the totally different tunica adventitia protein type in the same serotype strain, the strain which is different in serotype is also the separated strain of the same tunica adventitia protein; the fourth step produces bioplasm for generates seed-parent strain, selects two pairs of strains whose serotype and the tunica adventitia protein type are also different, and which is used as the filtering mark of coalescence, and they are used as the seed-parent strain for bioplasm coalescence; then producing bioplasm body, incubates the middle sized colony, and gets the product.

Description

technical field [0001] The invention belongs to the technical field of strain preparation, and relates to a preparation method of Escherichia coli heterotypic outer membrane protein fusion strain, which is especially suitable for chicken source. Background technique [0002] Vaccine immunoprophylaxis is currently an important means of controlling colibacillosis. The current development of chicken E. coli vaccines is based on serotyping. The monovalent serotype inactivated vaccines are only resistant to the same type of pathogenic bacteria, and the multivalent inactivated vaccines are the addition of representative strains of different serotypes. It is difficult to have both antigenic content and protective power. More than 60 serotypes of pathogenic Escherichia coli have been isolated from poultry in my country since the 1980s, and the serotypes in different provinces vary greatly. Although the inactivated Escherichia coli vaccine prepared by local strains has preventive e...

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Problems solved by technology

Although the inactivated Escherichia coli vaccine prepared by local strains has preventive effect, it has obvious geographical limitations, which limits the promotion of the vaccine. food reaction

[0003] According to statistics, there are 18 million laying hens and 60 million broiler chickens in Tianjin each year, and the annual stock of meat and laying hens in the country is as high as 10 billion. Because there is no vaccine that can be applied uniformly in various regions and the whole country, the incidence of colibacillosis in chickens is becoming more and more serious, and more and more getting harder to control,

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