Production method and preparation method of glucans

A technology for glucan and glucose, applied in the field of production of glucan and its derivatives, can solve the problems of undisclosed thermal stability of sucrose phosphorylase, cost and human safety, environmental problems are not good, prediction and the like

Inactive Publication Date: 2004-10-06
EZAKI GLICO CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Prior to the present invention, there was no publication regarding the effect of changing the concentration of sucrose in the reaction solution on the stability of sucrose phosphorylase, and no prediction of its effect
Furthermore, there is nothing disclosed about the thermostability of sucrose phosphorylase from Leuconostoc
Therefore, it is not possible to make predictions about any effect of utilizing this sucrose phosphorylase in glucan synthesis
[0016] The second problem is the operational problem
However, when producing amylose in large quantities industrially, the method of using organic solvents is not a good method in terms of cost, safety to human body, and environmental problems.
No method using non-organic solvents has been disclosed

Method used

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  • Production method and preparation method of glucans
  • Production method and preparation method of glucans
  • Production method and preparation method of glucans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2-1 to 2-5 and comparative Embodiment 2-1 to 2-7

[0368] (Examples 2-1 to 2-5 and Comparative Examples 2-1 to 2-7: Amylose is synthesized using a small amount of enzyme)

[0369] Synthesis of amylose was carried out using a reaction mixture whose composition (at the start of the reaction) is shown in Table 3 below.

[0370] Numbering

sucrose

(%)

G7

(mM)

Pi

(mM)

SP(U / g

sucrose)

GP(U / g

sucrose)

reaction

temperature

Comparative Example 2-1

4

2

20

5

5

37℃

Comparative Example 2-2

8

4

40

5

5

37℃

Comparative Examples 2-3

10

5

50

5

5

37℃

Comparative Examples 2-4

15

7.5

75

5

5

37℃

Comparative Examples 2-5

20

10

100

5

5

37℃

Comparative Examples 2-6

25

12.5

125

5

5

37℃

Comparative Examples 2-7

4

2

20

5

5

45℃

Example 2-1 ...

Embodiment 3-1-1 to 3-2-5 and comparative Embodiment 3-1-1 and 3-2-1

[0379] (Examples 3-1-1 to 3-2-5 and Comparative Examples 3-1-1 and 3-2-1: Synthesis of Amylose Using Thermostable Sucrose Phosphorylase)

[0380] Synthesis of amylose was carried out using a reaction mixture whose composition (at the beginning of the reaction) is shown in Table 4 below.

[0381] Numbering

sucrose

(%)

G7

(mM)

Pi

(mM)

SP(U / g

sucrose)

GP(U / g

sucrose)

reaction

temperature

comparative example

3-1-1

4

2

20

10

10

45℃

Example 3-1-1

8

4

40

10

10

45℃

Example 3-1-2

10

5

50

10

10

45℃

Example 3-1-3

15

7.5

75

10

10

45℃

Example 3-1-4

20

10

100

10

10

45℃

Example 3-1-5

25

12.5

125

10

10

45℃

comparative example

3-2-1

4

2

20

10

10

...

Embodiment 4-1-1 to 4-2-5， and comparative Embodiment 4-1-1 and 4-2-1

[0392] (Example 4-1-1 to 4-2-5, and comparative example 4-1-1 and 4-2-1: adopt a small amount of enzyme and thermostable sucrose phosphorylase to synthesize amylose)

[0393] Synthesis of amylose was carried out using a reaction mixture whose composition (at the start of the reaction) is shown in Table 5 below.

[0394] Numbering

sucrose

(%)

G7

(mM)

Pi

(mM)

SP(U / g

sucrose)

GP(U / g

sucrose)

reaction

temperature

comparative example

4-1-1

4

2

20

5

5

45℃

Example 4-1-1

8

4

40

5

5

45℃

Example 4-1-2

10

5

50

5

5

45℃

Example 4-1-3

15

7.5

75

5

5

45℃

Example 4-1-4

20

10

100

5

5

45℃

Embodiment 4-1-5

25

12.5

125

5

5

45℃

comparative example

4-2-1

4

2

20 ...

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Abstract

A first method for producing glucan comprises the step of allowing a reaction solution containing sucrose, a primer, inorganic phosphate or glucose-1-phosphate, sucrose phosphorylase, and glucan phosphorylase to react to produce glucans. The maximum value of the sucrose-phosphate ratio of the reaction solution from the start of the reaction to the end of the reaction is no more than about 17. A second method for producing glucan comprises the step of allowing a reaction solution containing sucrose, a primer, inorganic phosphate or glucose-1-phosphate, sucrose phosphorylase, and glucan phosphorylase to react to produce glucans. The reaction is conducted at a temperature of about 40 C to about 70 C.

Description

technical field [0001] The present invention relates to methods for producing dextran and its derivatives. More specifically, the present invention relates to methods of extending alpha-1,4-glucan chains. Background technique [0002] Dextran is a general term for a polysaccharide whose carbohydrate component consists only of D-glucose. Examples of representative glucans include starch, cellulose, and the like. Starches are alpha-glucans whose carbohydrate components are linked by alpha-glycosidic bonds. Amylose, that is, linear α-1,4-glucan, and amylopectin with a branched structure exist in starch. The ratio of amylose to amylopectin abundance varies depending on the plant that stores the starch. Therefore, it is difficult to obtain starch containing amylose and amylopectin in arbitrary composition ratios. If amylose can be produced stably, this amylose can be blended with commercially available starches to produce starches with any amylose co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C08B30/20C08B33/00C08B37/00C12P19/18C12R1/46
CPCC08B33/00C08B37/0009C12P19/18C08B30/20C12P19/04
Inventor 藤井和俊寺田喜信柳濑美千代大段光司高田洋树鹰羽武史栗木隆冈田茂孝
Owner EZAKI GLICO CO LTD
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