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Bovine mastitis resistant yolk antibody and its preparation method and formulation

A technology for egg yolk antibody and bovine mastitis, applied in anti-inflammatory agents, antibodies, non-central analgesics, etc., can solve problems such as economic losses

Inactive Publication Date: 2004-11-10
BIOINFORBODY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This will undoubtedly cause very large economic losses

Method used

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  • Bovine mastitis resistant yolk antibody and its preparation method and formulation

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Effect test

Embodiment 1

[0122] The anti-bovine mastitis yolk antibody of the present invention is detected according to non-denaturing polypropylene gel electrophoresis (separating gel concentration is 6.0%), and after Coomassie brilliant blue R250 staining, the pattern detection can present a single antibody at a molecular weight of 170-190KD. Band, Western Bloting detection can find a single specific band at the molecular weight of 170 ~ 190KD; or detect according to denatured polypropylene gel electrophoresis (separation gel concentration is 12.0%), after Coomassie brilliant blue R250 staining, the map There are two bands, Western Bloting detection can find two specific bands at the molecular weight of about 65KD and 25KD, the protein concentration is 0.1-20mg / ml measured by UV spectrophotometry; its titer is determined by enzyme-linked immunosorbent assay It is 1:50-1:100000.

[0123] The preparation method of the yolk antibody against bovine mastitis is completed according to the following steps...

Embodiment 2

[0189] The anti-bovine mastitis yolk antibody of the present invention is detected according to non-denaturing polypropylene gel electrophoresis (separating gel concentration is 6.0%), and after Coomassie brilliant blue R250 staining, the pattern detection can present a single antibody at a molecular weight of 170-190KD. Band, Western Bloting detection can find a single specific band at the molecular weight of 170 ~ 190KD; or detect according to denatured polypropylene gel electrophoresis (separation gel concentration is 12.0%), after Coomassie brilliant blue R250 staining, the map There are two bands, Western Bloting detection can find two specific bands at the molecular weight of about 65KD and 25KD, the protein concentration is 0.1-20mg / ml measured by UV spectrophotometry; its titer is determined by enzyme-linked immunosorbent assay It is 1:50-1:100000.

[0190] The preparation method of the yolk antibody against bovine mastitis is completed according to the following steps...

Embodiment 3

[0222] The anti-bovine mastitis yolk antibody of the present invention is detected according to non-denaturing polypropylene gel electrophoresis (separating gel concentration is 6.0%), and after Coomassie brilliant blue R250 staining, the pattern detection can present a single antibody at a molecular weight of 170-190KD. Band, Western Bloting detection can find a single specific band at the molecular weight of 170 ~ 190KD; or detect according to denatured polypropylene gel electrophoresis (separation gel concentration is 12.0%), after Coomassie brilliant blue R250 staining, the map There are two bands, Western Bloting detection can find two specific bands at the molecular weight of about 65KD and 25KD, the protein concentration is 0.1-20mg / ml measured by UV spectrophotometry; its titer is determined by enzyme-linked immunosorbent assay It is 1:50-1:100000.

[0223] The preparation method of the yolk antibody against bovine mastitis is completed according to the following steps...

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Abstract

The invention discloses a vitelline antibody for resisting bovine mastitis, its preparing process and preparation thereof, wherein hens capable of healthy oviposition are used as immune animal, and pathogenic bacteria causing bovine mastitis diseases are used as antigens, the preparation process comprises the steps of first immunization, reinforced immunization, collecting eggs and extracting bovine mastitis resistant vitellus antibody from vitelline, the preparation includes liquid preparation, solid preparation and semi-solid ointment preparation. The vitelline antibody for resisting bovine mastitis and its preparation can be used in the prevention and cure of bovine mastitis caused by pathogenic bacteria.

Description

technical field [0001] The invention relates to an anti-bovine mastitis yolk antibody and its preparation method and preparation. Background technique [0002] Cow mastitis is a common disease in dairy farming. With the rapid development of the dairy industry, the incidence of this disease has increased significantly. Among them, the incidence of dominant mastitis is as high as 20-40%, and the incidence of recessive mastitis is as high as 50-80%. . If the sick cows are not treated in time, it will lead to a sharp decrease in milk production, or even cause no milk, seriously affecting the production performance of dairy cows, which will bring huge economic losses to farmers. [0003] After scientific research and experiments, it has been proved that pathogenic microorganisms are the main pathogens causing mastitis, and environmental factors, management methods, and cattle body conditions are also related to the occurrence of this disease. The main pa...

Claims

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Application Information

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IPC IPC(8): A61K39/395A61P15/14A61P29/00C07K16/02C12P21/00
Inventor 叶志海王文
Owner BIOINFORBODY
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