Method for quickly detecting 4-methyl iminazole in food

A technology of methylimidazole and food, which is applied in the detection field of food additives, can solve the problems of lack of detection methods, high detection limit, low sensitivity, etc., and achieve the effects of easy popularization and use, simple operation and low price

Inactive Publication Date: 2005-01-05
CHINA AGRI UNIV
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AI Technical Summary

Problems solved by technology

Although the qualitative and quantitative analysis methods of caramel pigment have reached a certain level with the progress of analytical instruments in terms of accuracy and precision, the instrumental analysis methods require special analytical instruments and expensive testing costs
The problems in the method of silica gel thin-layer chromatography and paper chromatography for the determination of 4-methylimidazole con

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0013] 1-5 mL of soy sauce containing caramel pigment was filtered through a microporous membrane and added to a pretreated and activated XTR 1000 mg solid-phase extraction column, and the 4-methylimidazole mixture was eluted with organic solvent ethyl acetate or dichloromethane to obtain the tested The sample solution was directly sampled after concentration.

[0014] Use a micro-spotter to spot 3 to 6 μL of sample on a 2×8 cm polyamide sheet, put it vertically into a closed development cylinder filled with a developer (cyclohexane:acetone:ethanol=5:1:0.7) and move upward The expansion distance is 7-7.5cm. Compared with the 4-methylimidazole standard on the same thin-layer plate, it can be determined whether the target compound exists in the soy sauce product, and the target compound and impurities are completely separated under this chromatographic condition. separate. Take out the thin plate, evaporate the solvent, develop the color with iodine vapor, conduct qualitative d...

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PUM

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Abstract

The invention discloses a method for detecting the 4-methyl imidazole in food quickly belonging to food additives detection range. The sample containing caramel pigment is extracted in solid phase through solid phase extraction pile (XTR is more than 500mg), after the sample passes through the pile, it is rinsed with organic solvent, the purged liquid is condensed, uses micro sample applicator to spot sample directly on the chromatography material polyamide film, and it is developed in the developing agent with the same proportion, observes it with naked eye after it is color developed with iodine steam, it observes the brown spots with the rf values the same to the standard sample in rf range between 0.5 and 0.7, then considers that the measured material has 4-methyl imidazole. It can detect if the measured sample is natural or is confected manually, especially proper to detect the sample such as sauce, vinegar and cola containing caramel pigment.

Description

technical field [0001] The invention belongs to the detection scope of food additives, and in particular relates to a method for rapidly detecting 4-methylimidazole in food which can be used as a food production factory to detect caramel pigment and food containing caramel pigment. Background technique [0002] Caramel coloring is a food coloring widely used in the world, with an annual consumption of about 200,000 tons worldwide, of which more than 90% are used in food and beverages. About 95% of caramel coloring in the United States is used in beverages; caramel coloring in Asia is mainly used in soy sauce and other condiments. For example, soy sauce is an indispensable traditional condiment in the life of our people. With the improvement of people's living standards, in addition to requiring soy sauce to have certain nutritional components and sensory quality, higher requirements have been put forward for the safety and hygiene of soy sauce. Color is one of the most impo...

Claims

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Application Information

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IPC IPC(8): G01N30/90
Inventor 陈敏郭小莉戴蕴青何计国
Owner CHINA AGRI UNIV
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