Method for preparing transparent calcium hyaIuronate in low molecular weight

A technology of calcium hyaluronate and low molecular weight, applied in the field of preparation of calcium hyaluronate, can solve the problems of poor stability, long cycle, complicated processing technology and the like

Inactive Publication Date: 2005-01-12
CHINA EASTAR HLDG GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After being exchanged with calcium salt, the obtained calcium hyaluronate has low solubility in aqueous solution due to its large molecular weight and poor stability, and it is easy to precipitate when placed
The current method only uses one of the two characteristics of acid instability and thermal instability of hyaluronic acid to degrade hyaluronic acid and then form calcium salt. This technology has many process steps and complex processing technology. Long cycle, about 2-4 days, difficult to control, high processing cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Take 30g of crude sodium hyaluronate (viscosity average molecular weight about 1.8×106-2.0×106 Daltons), dissolve it in 3000ml deionized water, adjust the pH value to 4.3-4.7, heat to 60-70°C, fully dissolve, keep stirring 2-3 hours; add 6-10g of activated carbon, fully stir for 0.5 hours; use diatomaceous earth to filter the solution, cool the filtrate to room temperature, adjust the pH value of the filtrate to 4.3-4.7, and pass through the strong acid cation exchange resin (001×7) smoothly, The pH value of the exchange solution is 1.8; add 7.0g of calcium chloride CaCl to the exchange solution 2 (the molar ratio is about 1.25), heated to 60-70°C and stirred for 2 hours, and then used calcium hydroxide Ca(OH) 2 Adjust the pH value to 4.3-4.7; filter the reaction solution to remove solid insoluble matter, concentrate under vacuum at 60-70°C to 30ml; add 0.3g activated carbon to the concentrated solution for decolorization at 60-70°C for 30 minutes, filter; cool the filt...

Embodiment 2

[0010] Take 20g of crude sodium hyaluronate (viscosity average molecular weight about 1.2×106-1.4×106 Daltons), dissolve it in 2000ml deionized water, adjust the pH value to 4.3-4.6, heat to 60-65°C, fully dissolve, keep warm and stir 2-3 hours; add 4-6g of activated carbon, stir well for 0.5 hours; the solution is filtered with kaolin, the filtrate is cooled to room temperature, the pH of the filtrate is adjusted to 4.3-4.5, and the filtrate is passed smoothly through a membrane filter with a cut-off molecular weight of 5000-10000, fully concentrated and desalted ;Add 4.5g calcium chloride CaCl to the exchange solution 2 (the molar ratio is about 1.2), heated to 60-70°C and stirred for 1.5 hours, and then used calcium hydroxide Ca(OH) 2 Adjust the pH value to 4.5-4.7; filter the reaction solution to remove solid insoluble matter, concentrate under reduced pressure at 60-70°C to 20ml; add 0.15g activated carbon to the concentrated solution for decolorization at 60-70°C for 30 ...

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Abstract

The invention discloses a method for preparing calcium hyaluronate with low molecular weight. Said method utilizes the acid instability and thermal instability of hyaluronic acid to make the molecular weight of the hyaluronic acid by degraded into 5000-10000, then adds the inorganic calcium salt to make the hyaluronic acid be converted into the calcium hyaluronate, then utilizes the organic solvent obtained by mixing ethyl alcohol and acetone, etc. with water to make the calcium hyaluronate be precipitated and separated out. The technological process of said preparation method is relatively simple, its period is short, and is about 10 hr. and it is suitable for industrial large scale production. Said calcium hyaluronate has high water solubility, and aqueous solution stability.

Description

1. Technical field [0001] The invention relates to a preparation process of calcium hyaluronate, in particular to a method for preparing low molecular weight calcium hyaluronate. 2. Technical background [0002] At present, the methods for obtaining calcium hyaluronate are mainly fermentation or extraction, and hyaluronic acid obtained from fermentation or extraction is in the form of its sodium salt, and its molecular weight is generally greater than 5×10 5 . After being exchanged with calcium salt, the obtained calcium hyaluronate has low solubility in aqueous solution due to its large molecular weight and poor stability, and it is easy to precipitate out when placed. The current method only uses one of the two characteristics of acid instability and thermal instability of hyaluronic acid to degrade hyaluronic acid and then form calcium salt. This technology has many process steps and complex processing technology. The cycle is long, about 2-4 days, it is difficult to co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
Inventor 阮春学
Owner CHINA EASTAR HLDG GRP
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