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Alkaline phosphatase detecting reagent

A technology for detecting reagents and phosphatase, applied in the field of diagnostic reagents, can solve problems such as large differences between reagent batches, missed diagnosis, misdiagnosis, etc., and achieve the effect of long open bottle validity period and long shelf life

Inactive Publication Date: 2005-03-09
DIASYS DIAGNOSTIC SYST SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The present invention aims to solve the common problems in the current alkaline phosphatase detection reagents that there are large differences between reagent batches, and the interfering substances contained in the detection samples have relatively large interference with the detection. Especially in clinical use, the existence of these problems brings many uncertainties to doctors in diagnosing the disease. technical problems that are likely to cause misdiagnosis and missed diagnosis

Method used

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  • Alkaline phosphatase detecting reagent
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Experimental program
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Effect test

Embodiment 1

[0035] Glycine and sodium lauryl sulfate were added to the reaction substrate at a concentration of 0.1mmol / L and 3mmol / L respectively as finished reagent 2 as a stabilizer, and the pH was adjusted to 7.5 with Good’s buffer. (hereinafter referred to as D reagent)

Embodiment 2

[0037] Glycine and sodium lauryl sulfate were added to the reaction substrate at a concentration of 1mmol / L and 17mmol / L respectively as finished reagent 2 as a stabilizer, and the pH was adjusted to 11.0 with Good’s buffer. (hereinafter referred to as E reagent)

Embodiment 3

[0039] Glycine and sodium lauryl sulfate were added to the reaction substrate at concentrations of 0.5mmol / L and 10mmol / L respectively as finished reagent 2 as a stabilizer, and the pH was adjusted to 9.0 with Good’s buffer. (hereinafter referred to as F reagent)

[0040] The above three reagents D, E, and F were tested separately, and the results are shown as follows:

[0041] Table 5 Reagent blank absorbance results (28 days)

[0042]

[0043] Table 6 reagent detection control product results (28 days)

[0044]

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Abstract

A reagent for detecting alkaline phosphotase is prepared from p-nitrophenol phosphate as substrate through proportionally mixing it with glycine and sodium laurylsulfate and regulating pH-7.5-11 by Good's buffer liquid.

Description

Technical field: [0001] The invention relates to a diagnostic reagent, in particular to an alkaline phosphatase detection reagent which uses p-nitrophenol phosphate as a reaction substrate. Background technique: [0002] my country is a big country with hepatitis. According to statistics from the National Center for Disease Control and Prevention, hepatitis B virus infection and carriers in my country account for 10 to 14% of the total population. Rickets in children is one of the "four diseases in children" that my country focuses on. Biliary tract, orthopedics, etc. The incidence of disease is also relatively high. Alkaline phosphatase, as the basic diagnostic method for biliary tract, bone, liver and other diseases, is widely used in clinic because of its simplicity, rapidity, specificity and trace amount. [0003] Some common methods for alkaline phosphatase detection are as follows: [0004] 1. Use p-nitrophenol phosphate as the reaction substrate. Under alkaline condi...

Claims

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Application Information

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IPC IPC(8): C12Q1/42
Inventor 钱震斌丁耀良巢宇
Owner DIASYS DIAGNOSTIC SYST SHANGHAI
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