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Banana endophyte and its use

A technology of endophytic bacteria and bananas, applied in the direction of bacteria, etc., can solve the problems that there are not many studies on endophytic actinomycetes, and there is no test report on banana endophytic Streptomyces resistance to banana wilt

Inactive Publication Date: 2005-07-13
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are not many studies on endophytic actinomycetes in the world, and there is no test report on banana endophytic streptomyces for resisting banana wilt

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] One of the isolation and culture methods of Streptomyces CCTCC M203070:

[0029] 1. Collect the root samples of healthy banana plants in banana wilt disease-affected areas, fully rinse, process with 70% ethanol for 5 minutes, process with sodium hypochlorite (0.9% available chlorine) solution for 20 minutes, and fully rinse with sterile water;

[0030] 2. Cut the processed samples into pieces and insert them into the S plate;

[0031] 3. Place the plate at 26°C for 5 days;

[0032] 4. Pick the characteristic colonies of actinomycetes with compact mycelia, streak them on the same medium plate, purify and separate them, and culture them at 24-30°C for 5 days, pick a single colony, and store them on the slant of the same medium.

[0033] 5. The plate confrontation test was carried out between the obtained pure strain and the pathogen Fusarium oxysporum, from which the strain with resistance to the pathogen was selected, and after further verifying its growth-promoting and...

Embodiment 2

[0035] The second method of isolation and culture of Streptomyces CCTCC M203070:

[0036] 1. Collect leaf samples from healthy banana plants in banana wilt-affected areas, rinse thoroughly, treat with 36% formaldehyde for 30 seconds, treat with sodium hypochlorite (0.9% available chlorine) solution for 20 minutes, and fully rinse with sterile water;

[0037] 2. Cut the processed samples into pieces and insert them into the improved S plate;

[0038] 3. Place the plate at 26°C for 7 days;

[0039] 4. Pick the characteristic colonies of actinomycetes with compact mycelia, streak and separate them on the same medium plate, culture them at 24-30°C for 5 days, pick a single colony, and store them on the slant of the same medium.

[0040] 5. The obtained pure bacterial strain and the pathogenic bacteria Fusarium oxysporum were used as a plate confrontation test, and the bacterial strain with resistance to the pathogenic bacteria was selected therefrom. After further verifying its g...

Embodiment 3

[0042] One of the cultivation seed production method of Streptomyces CCTCC M203070:

[0043] 1. the streptomyces CCTCC M203070 pure species that embodiment 1 or 2 obtains is connected to the S medium slant;

[0044] 2. Cultivate at 28°C until the spores are full;

[0045] 3. Transfer to the same medium plate and culture at 28°C until the spores are full; the spores can be directly used to inoculate bananas or expand culture.

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PUM

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Abstract

The present invention relates to banana endogenous streptomycete, its separation and culture method and its use in promoting banana growth and preventing and treating banana fusarium blight. The banana endogenous streptomycete CCTCC M203070 may be obtained from banana plant in banana blight area through separation and culture. Experiment shows that the streptomycete CCTCC M203070 has the functions of promoting banana seedling growth and raising the resistance to fusarium blight, so that it may be used in promoting banana growth and preventing and treating banana fusarium blight.

Description

technical field [0001] The invention relates to a plant endophytic actinomycete-banana endophytic streptomyces and its isolation and cultivation method, and its use for promoting growth of bananas and preventing Fusarium oxysporum (Fusarium oxysporum) wilt. Background technique [0002] Plant endophytes are microorganisms that can be isolated from surface-sterilized plant tissues or obtained from the sap inside plants, and are not harmful to plants in appearance, that is, they do not cause obvious diseases. Plant endophytes include mutualistic and neutral endosymbiotic microorganisms. At present, endophytes have been isolated from various organs (roots, stems, leaves, flowers, fruits, seeds) of many plants. Their ubiquity, rich biological diversity and functional diversity in plants have been reported in many literatures. Endophytes interact with plants for a long time, and they form a more complex relationship. Endophytes have been found to have the following effects on ...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 周世宁曹理想
Owner SUN YAT SEN UNIV
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