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Quick determining method for period of pasteruzied milk on counter

A pasteurization and detection method technology, applied in the fields of biochemical equipment and methods, testing food, material inspection products, etc., can solve the problem that it takes several months or even 1-2 years, the impedance method is difficult to measure, and it is difficult to adapt to rapid detection. and other problems to achieve the effect of rapid prediction

Inactive Publication Date: 2005-07-27
SOUTH CHINA UNIV OF TECH
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  • Abstract
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Problems solved by technology

However, the traditional impedance method reported in Peter K, Ruth F E. Prediction of shelf-life of pasteurized milk and other fluid dairy products in 48 hours. J. Dairy Sci., 1987, 70: 1544~1550 is mainly aimed at psychrophilic bacteria. The pre-cultivation process at 18°C ​​and 18h ​​took 48h to predict the shelf life
[0004] Since the detection sensitivity of the impedance method is 10cfu / mL, and the bacteria content in the freshly produced pasteurized milk is often lower than this concentration, it is difficult to directly measure the impedance method
Moreover, since the traditional impedance method needs to establish a standard curve between the number of bacteria and DT, and then perform the conversion determination of the shelf life, the establishment of the standard curve generally requires more than 100 pairs of valid data with a span of 4 to 5 orders of magnitude. It takes several months or even 1 to 2 years, and it is difficult to adapt to the requirements of rapid detection

Method used

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  • Quick determining method for period of pasteruzied milk on counter
  • Quick determining method for period of pasteruzied milk on counter

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Prediction of Shelf Life of Xiangmanlou Pasteurized Milk

[0030] From the day of production, the samples were stored at 4°C, and one sample was taken every day for testing. Half of the samples were tested for shelf life, and the other half of the samples were pre-cultured as follows before impedance measurement: no pre-culture; Impedance measurement, the result is as figure 1 shown.

[0031] From figure 1 A It can be seen that with the sample volume of 100-400 μL, some DT values ​​of the impedance detection instrument on the non-pre-cultured milk samples failed to appear, and the obtained DT time values ​​were large and varied irregularly, so it was difficult to predict the shelf life. .

[0032] Compared with no pre-culture, the DT time measured by pre-culture at 30°C for 6 hours becomes shorter. In the range of 100-400 μL sample volume, there is a certain correlation between shelf life and DT time, that is, the longer the shelf life, the longer the DT time. The ...

Embodiment 2

[0090] Popular pasteurized milk shelf life forecast

[0091]The popular pasteurized milk samples were subjected to three pre-culture treatments: no pre-culture, 6h pre-culture at 30°C, and 6h pre-culture at 37°C, and 100-400μL samples were taken every day for DT determination. The result is as figure 2 shown.

[0092] From figure 2 A. It can be seen that with the sample volume of 100-400 μL, the DT time value obtained by the impedance detection instrument on the non-pre-cultured milk sample is large and irregular, and it is difficult to predict the shelf life. It is estimated that the number of bacteria in the milk sample without pre-culture is less, especially when the potential shelf life of the milk is longer, the number of bacteria in the milk is less, and it will occasionally not appear during the set 24h test time DT detection time.

[0093] Compared with no pre-culture, pre-culture at 30°C for 6 hours ( figure 2 B) The measured DT time becomes shorter, and withi...

Embodiment 3

[0157] Yantang pasteurized milk shelf life test

[0158] The Yantang pasteurized milk samples were pre-incubated at 30°C, 6h or 37°C, 6h respectively, and 100-400 μL was sampled for impedance detection. Compared with the actual shelf life, the results are shown in Table 8.

[0159] Table 8 Detection of shelf life of Yantang pasteurized milk

[0160] Batch Pre-culture Sample volume / μL DT / h Estimated shelf life / d Measured shelf life / d

[0161] 1 30℃, 6h 300 6.8 1.94 2.0

[0162] 400 6.4 2.33

[0163] 37℃, 6h 100 5.9 2.39

[0164] 200 5.3 1.77

[0165] 300 5.1 1.71

[0166] 400 5.1 2.35

[0167] 2 30℃, 6h 300 7.9 6.10 6.0

[0168] 400 7.2 6.14

[0169] 37℃, 6h 100 6.7 6.29

[0170] 200 6.2 5.98

[0171] 300 5.8 5.99

[0172] 400 5.9 6.16

[0173] 3 30℃, 6h 300 8.7 8.43 8.0

[0174] 400 7.7 8.11...

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Abstract

A fast detection method includes preculturing sample of pasteurization milk, setting up fast detection standard curve and prediction equation for selflife of pasteurization milk, measuring impedance of pasteurization milk and detecting selflife of pasteurization milk.

Description

technical field [0001] The invention relates to a method for rapidly detecting pasteurized milk, in particular to a method for rapidly detecting the shelf life of pasteurized milk. Background technique [0002] Pasteurized milk refers to milk products that pass pasteurization process after fresh milk is packaged, and are heated at 72°C to 75°C for 15 to 20 seconds to control the number of bacteria within 30,000 per milliliter as stipulated by the state. Because its taste and flavor are similar to fresh milk and consume less nutrients, it has gradually become a popular daily consumer product. Its biggest advantage is to keep the fresh taste of liquid milk. At present, pasteurized milk packaging occupies a large market share in the milk-rich regions of the world. Since pasteurized milk still contains a certain amount of bacteria, its freshness period is very short, and it can only be kept fresh for about a week in an environment below 4°C. Therefore, the requirements for sto...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06G01N27/04G01N33/04
Inventor 王菊芳李志勇
Owner SOUTH CHINA UNIV OF TECH
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