Oligonucleotide series for calculation of DNA chip

Abstract

A group of oligonucleotide sequences used for the calculation of DNA chip features that the length of its each sequence is 12 bases, and its each sequence can meet such restrictive conditions that 22 mother sequences are generated, the content of G+C is 90%, and the ratio of any sequence to another sequence is not greater than 4.

Description

technical field [0001] The present invention relates to an oligonucleotide sequence in the field of gene technology, in particular to an oligonucleotide sequence used in a DNA computing chip. Background technique [0002] DNA computing is a new field developed in recent years. In 1994, Adleman (Science, 1994, 266: 1021-1024) used DNA sequence as the information carrier for the first time, and used molecular biology test technology to solve the Hamilton path problem of the seven vertices in the test tube. Later, many scholars proposed a variety of DNA calculation models for different NP problems, and these DNA calculations are all calculations in the solution system. Although a variety of DNA calculations can be realized in the solution, there are still some insurmountable problems in the reaction in the solution, such as the difficulty of separating intermediate products, the complexity of the reaction system, the difficulty of monitoring the intermediate process, the poor ...

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Problems solved by technology

However, for the hybridization reaction on the surface of the chip, due to the requirement of the hybridization stability of the oligonucleotide sequence, the sequence length usually needs to exceed 12 bases. If it is lower than this length, the hybridization stability is poor and the signal is weak.

Although Frutns et al. designed 108 sequences, the length of the variable region of these sequences is only 8 bases. If they are used for DNA chip calculations, they cannot guarantee hybridization stability and no hybridization mismatches at the same time.

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