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Method for preparing cinobufotalin lyophilized powder

A technology of freeze-dried powder injection and cinobufacin, which is applied in the field of cinobufacin extraction process and freeze-dried powder injection preparation, which can solve the problems of fragility, abnormal heart rhythm and toxic side effects

Inactive Publication Date: 2006-05-10
郭斌阁
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Injections have high requirements on stability, and the inherent defects of aqueous injections are stability problems and easy breakage during carrying, storage, and transportation, which brings a lot of inconvenience
[0026] Chinese Patent (Application No. 200410083994.4) "A Cinobufacin Freeze-Dried Powder Injection and Its Preparation Method" describes the use of ethanol to extract active ingredients to make powder injections. The inventive process contains a large amount of fat-soluble ingredients. Abnormal heart rhythm and other side effects, and macromolecules that may cause allergen allergies have not been effectively removed in the process

Method used

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  • Method for preparing cinobufotalin lyophilized powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0139](1) The raw material is: dry toad skin 1000g;

[0140] (2) Take dry toad skin, wash it, add 10 times and 8 times distilled water, extract with warm heat, the first time is 30 minutes, the second time is 30 minutes, combine the water extracts, filter, and the filtrate is concentrated to contain 1g crude drug / ml, centrifuged at 3000r / min for 20 minutes, took the supernatant, and ultrafiltered it with a 3000 dalton ultrafiltration membrane to obtain toad skin water extract;

[0141] (3) Preparation prescription: 25 mg of cinobufacin aqueous extract, 90 mg of mannitol.

[0142] (4) Mix the above-mentioned cinobufacin water extract with pharmaceutical excipients, add water for injection to dissolve, add water for injection to a specified amount, adjust the pH value to 6.5-7.0, sterilize and filter, fill, and freeze-dry to obtain the product.

Embodiment 2

[0144] (1) The raw material is: dry toad skin 1000g;

[0145] (2) Take dry toad skin, wash it, add 10 times and 8 times distilled water, extract with warm heat, the first time is 30 minutes, the second time is 30 minutes, combine the water extracts, filter, and the filtrate is concentrated to contain 1g crude drug / ml, centrifuged at 3000r / min for 20 minutes, took the supernatant, and ultrafiltered it with a 4000 dalton ultrafiltration membrane to obtain the water extract of toad skin;

[0146] (3) Preparation prescription: 25 mg of cinobufacin aqueous extract, 90 mg of dextran.

[0147] (4) Mix the above-mentioned cinobufacin water extract with pharmaceutical excipients, add water for injection to dissolve, add water for injection to a specified amount, adjust the pH value to 6.5-7.0, sterilize and filter, fill, and freeze-dry to obtain the product.

Embodiment 3

[0149] (1) The raw material is: dry toad skin 1000g;

[0150] (2) Take dry toad skin, wash it, add 10 times and 8 times distilled water, extract with warm heat, the first time is 30 minutes, the second time is 30 minutes, combine the water extracts, filter, and the filtrate is concentrated to contain 1g crude drug / ml, centrifuged at 3000r / min for 20 minutes, took the supernatant, and ultrafiltered it with a 4000 dalton ultrafiltration membrane to obtain the water extract of toad skin;

[0151] (3) Preparation prescription: 50 mg of cinobufacin aqueous extract, 90 mg of dextran.

[0152] (4) Mix the above-mentioned cinobufacin water extract with pharmaceutical excipients, add water for injection to dissolve, add water for injection to a specified amount, adjust the pH value to 6.5-7.0, sterilize and filter, fill, and freeze-dry to obtain the product.

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Abstract

The invention discloses a process for preparing Cinobufacini freeze dried injection, which is prepared from indole alkaloid significant part extracted from dried toad peels and medicinal adjuvant through freeze drying, the Cinobufacini significant part is prepared through warm water extraction, centrifuging, and ultrafiltration purification.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine pharmacy, and in particular relates to a cinobufacin extraction process and a preparation method of freeze-dried powder injection thereof. Background technique [0002] Cinobufotalin is the whole skin extraction preparation of Bufobufo gargarizans Cantor or B.melanostictus Schneider, etc., which mainly contains active ingredients such as toad toxin, and has the functions of clearing away heat and detoxifying, diuretic and diuretic. swelling, blood stasis ulceration and other effects. Toad has been used as a medicine to treat diseases for a long time. Its medicinal properties are theoretically recorded in the first pharmacology monograph "Shen Nong's Materia Medica": "Toad, spicy and cold, dominates evil spirits, breaks lame hard blood, carbuncle, yin Sores, taking it will not cause fever." The name "Toad" was first seen in "Bielu of Famous Doctors", and Tao Hongjing noted th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/56A61K9/19A61K35/65A61P1/16A61P11/02A61P17/06A61P31/20A61P31/22A61P35/00
Inventor 郭斌阁
Owner 郭斌阁
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