Bacterial-breaking process druing procedure of BCG acid powder polysaccharide and nucleic

Abstract

Bacterium-breaking process in preparing of Calmette-Guerin bacterium and nucleic acid primitive powder is carried out by shearing, dispersing and emulsifying. It costs low, has more nucleic acid content and better quality.

Description

technical field [0001] The invention relates to a new process for destroying bacteria in the preparation process of BCG polysaccharide and nucleic acid raw powder, in particular to destroying bacteria of BCG cells by a high-shear dispersion emulsification method. Background technique [0002] BCG polysaccharide and nucleic acid (BCG Polysaccharide and Nucleic) can regulate the immune function of the human body, treat and prevent diseases such as colds, asthma, chronic dampness and chronic idiopathic urticaria. [0003] The existing whole process of preparing BCG polysaccharide and nucleic acid raw powder is culture medium preparation, inoculation culture, bacteria collection, bacteria destruction, extraction, refining and preparation of dry powder. Wherein the bacteriostasis process is a key step in the whole preparation process, and the quality of bacteriostasis seriously affects the yield and quality of the product. At present, domestic manufacturers generally use high-sp...

AI Technical Summary

Problems solved by technology

[0005] However, in the existing preparation process, due to the eddy current formed by the bacteriostasis solution during the rotation of the cutter, the contact between the cutter and the thalline is not sufficient, and the simple cutting is between the cutter and the thalline, so the bacterium destruction is not thorough enough, and the yield of raw powder is low. Only about 2%, the nucleic acid content is 10% ± 1%, which is significantly lower than the standard range of 10-20% in the "2000 Regulations for Biological Products"

Images