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Process for extracting N6-(2-ethoxyl) adenosine from Paecilomyces Cicadae Samson culture

A technology of Paecilomyces cicadae and Paecilomyces cicadae strains, applied in chemical instruments and methods, drug combinations, sugar derivatives, etc.

Inactive Publication Date: 2006-09-06
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In summary, the analgesic active substance of Paecilomyces cicadae and its preparation method and utilization have not been reported yet. Therefore, the analgesic chemical components of Paecilomyces cicadae and their extraction methods are clarified, and then developed into a new type of safe analgesic Pain drugs have important social and economic benefits

Method used

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  • Process for extracting N6-(2-ethoxyl) adenosine from Paecilomyces Cicadae Samson culture
  • Process for extracting N6-(2-ethoxyl) adenosine from Paecilomyces Cicadae Samson culture
  • Process for extracting N6-(2-ethoxyl) adenosine from Paecilomyces Cicadae Samson culture

Examples

Experimental program
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Effect test

Embodiment 1

[0124] Embodiment 1: (solid culture fermentation method " 1 ")

[0125] Take the APC-20 strain stored in the refrigerator at 4°C and transplant it to the slant culture medium (Note 1) for 7 days, and activate it for use; each 500ml triangular bottle contains 200ml of the first-grade seed medium (Note 2), and insert the slant strain 3 rings of spore powder, placed in a DHZ-C oscillator at 25°C, 120r / min, and cultivated for 4 days as first-grade seeds; put 7.5L of secondary medium in a 10L fermenter (Note 3), and sterilize at 121°C for 30min Cool to 24°C, insert first-grade seeds according to 10% inoculation amount, at 24°C, tank pressure is about 0.04Mpa, air flow is about 0.75m 3 / h, fermented for 48 hours as secondary seeds. After mixing the solid medium (Note 4) and water at a ratio of 1:2, one method can be directly loaded into the culture container, preferably about 2 / 5 of the capacity, placed in a high-pressure steam boiler, heated at 121°C , after 0.11Mpa sterilization ...

Embodiment 2

[0130] Embodiment 2: (solid culture fermentation method " 2 ")

[0131] The solid fermentation medium of the present embodiment is bran: corn: bran in a ratio of 2: 1: 1; the solid medium dry material and water are mixed in a ratio of 1: 2.9 weight components;

[0132] The inoculation amount of secondary strains for closed container culture is 3%; the inoculum amount of secondary strains for open culture in curved disc is 10%;

[0133] The culture medium and other processes and steps of this embodiment are the same as those in Example 1.

Embodiment 3

[0134] Embodiment 3: (solid culture fermentation method " 3 ")

[0135] Use wheat grains as the solid medium, first soak the grains in water overnight, steam them (until the grains crack), and put them into a container or a bent plate to occupy one-third of the volume; the secondary or tertiary bacteria cultured in closed containers The seed inoculum amount is 3%; the strain inoculum amount of the open culture of the curved disc is 10%; the rest of the culture process and steps are the same as in Example 1.

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Abstract

The inventin is named a method for extraction of N6-(2-hydroxyethyl) adenosine from paecilomyces cicadae culture. The invention consists of seperating and breeding new bacterial strain APC-20 of paecilomyces cicadae which is of strong livabitity, high yield of metabolically active compound and good quality from the nature; presenting a method for industrialized solid culture, fluid culture or fermentation production; then eatracting analgesic compounds contained in the said fermented product with 0.36 % of extraction rate and every significant part and providing a method for preparing polytypic species of analgesic drug; the compound has 99 % of analgesic rate examined by little white mouse-twist method. The analgesic mechanism of this compound is different from the presently used opium analgesic agent, and is of no addiction and safe, so it has a broad development and application propect.

Description

[0001] This application is a divisional application of the original application date: October 30, 2004, application number: 200410094511.0, and the invention name is "artificial cultivation of new strain of Paecilomyces cicadae and extraction and utilization of analgesic compound". technical field [0002] The present invention relates to the field of extracting effective parts of Cordyceps fungus fermentation products, in particular to an artificial solid or liquid fermentation culture of Paecilomyces cicadae new strain APC-20, from which the analgesic compound N is extracted. 6 -(2-hydroxyethyl)adenosine method. Background technique [0003] Cicadae has been a precious Chinese medicinal material with high medicinal value since ancient times. It is a complex parasitic on cicada nymphs by Paecilomyces cicadae (genus Cordyceps). In the past, people mainly collected rare natural cicadae for various health care products or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H19/16C07H1/06C12N1/14C12P1/02C12P21/02A61K9/00A61P43/00
Inventor 柴一秋韦忠民陈祝安厉晓腊刘又高王根锷
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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