Unlock instant, AI-driven research and patent intelligence for your innovation.

Extracellular potential measuring device and method for fabricating the same

A manufacturing method, cell technology, applied in the direction of measuring devices, electrochemical variables of materials, biochemical cleaning devices, etc.

Inactive Publication Date: 2006-09-20
PANASONIC CORP
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the fragment-clamping method requires special techniques in the production and handling of the micropipette, and since it takes a lot of time to measure one sample, it is not suitable for high-speed screening of a large number of drug candidate compounds.
In addition, a large number of drug candidate compounds can be screened at high speed using methods such as fluorescent dyes. However, the process of staining cells is necessary. S / N is also bad

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Extracellular potential measuring device and method for fabricating the same
  • Extracellular potential measuring device and method for fabricating the same
  • Extracellular potential measuring device and method for fabricating the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0059] figure 1 is a perspective view of the extracellular potential measuring device according to Embodiment 1 of the present invention, figure 2 is its cross-sectional view, image 3 is an enlarged view of its cross-section.

[0060] This extracellular potential measuring device includes a substrate 1 made of silicon on which grooves 2 are provided. A plurality of cell trapping holes 101 are provided on the bottom surface 3 of the groove 2, and each trapping hole 101 is constituted by a first opening 4, a cavity 6, and a second opening 5 sequentially connected on a straight line. The diameter of the first opening 4 is smaller than the maximum diameter of the cavity 6 and larger than the diameter of the second opening 5 .

[0061] These specific dimensions are optimally determined according to the size of the subject cells. For example, in a subject cell with a diameter of 25 microns, the diameter of the first opening 4 is 20 microns, which is smaller than 25 microns, and ...

Embodiment approach 2

[0094] 23 is a perspective view of an extracellular potential measurement device according to Embodiment 2 of the present invention, Figure 24 is its cross-sectional view, Figure 25 is its enlarged cross-sectional view. Unlike Embodiment 1, substrate 16 is a laminate of first silicon layer 17 , silicon dioxide layer 18 , and second silicon layer 19 . The first opening 21 is formed on the silicon layer 17 , the second opening 22 is formed on the second silicon layer 19 , and the cavity 23 is formed on the silicon dioxide layer 18 .

[0095] The detection electrode 24 is formed only on the inner wall of the second opening 22 and below the cavity 23 , and the extraction electrode 25 is formed on the bottom of the substrate 16 . The detection electrode 24 and the extraction electrode 25 are electrically connected near the second opening 22 .

[0096] Since the operation of this extracellular potential measuring device is the same as that of the measuring device in Embodiment ...

Embodiment approach 3

[0106] Figure 30 is a perspective view of an extracellular potential measuring device according to Embodiment 3 of the present invention, Figure 31A , Figure 31B as well as Figure 32 is its cross-sectional view. also, Figure 33 ~ Figure 34 is an enlarged cross-sectional view of the measurement setup. Figure 35 ~ Figure 42 is a cross-sectional view for explaining the manufacturing method of the measuring device.

[0107] exist Figure 30 ~ Figure 32 The substrate 28 is composed of a laminated structure of a base 29 made of silicon, an intermediate layer 30 made of silicon dioxide, and a thin plate 31 made of silicon. A sample containing subject cells is stored on the substrate 29, a groove 32 for mixing the subject cells 37, the culture solution 38, and a drug is provided, and a through hole is provided on the thin plate 31 forming the bottom of the groove 32. 33. A recess 34 is provided on the side of the groove 32 of the through hole 33 , and the diameter of the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
diameteraaaaaaaaaa
depthaaaaaaaaaa
Login to View More

Abstract

The invention relates to a device for measuring the cell external electric potential of somatic cell, which is characterized in that: it comprises base plate with a bottom, a middle layer above said bottom and formed by different material, and a sheet above the middle layer and formed by the same material as bottom. From said bottom, said middle layer has the groove with a bottom, from the bottom to the middle layer; and said sheet has the through cut through to the groove and outside the base plate.

Description

[0001] (This application is a divisional application of the application with the same name filed on June 2, 2003 with application number 03801204.9) technical field [0002] The present invention relates to an extracellular potential measuring device and a manufacturing method thereof for simple and high-speed electrophysiological evaluation using electrochemical changes in living samples, especially cells, as indicators. Background technique [0003] At present, the electrical activity of cells is used as an indicator, and the drug is screened by a patch clamp method, or by using a fluorescent dye or a luminescent indicator drug. [0004] In this fragment clamping method, ion transport of a minute portion of a patch called a cell membrane attached to the tip of a micropipette through a single-channel protein molecule is electrically recorded by a microelectrode probe. This method is one of the few methods that can investigate the function of a protein molecule in real time ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26C12M1/34C12Q1/02G01N27/30G01N27/416G01N33/483G01N27/28
Inventor 中谷将也冈弘章江本文昭
Owner PANASONIC CORP