Method for inducing human embryo stem cell differentiation to liver cell and the special-purpose medium

Abstract

The invention discloses a method of inducing the human embryo stem cell differentiation to liver cell and its special culture medium. The culture medium comprises the following two culture mediums: differentiation medium I which is prepared by adding 80-120ng / L activin A in RPMI 1640 culture medium and differentiation medium II which is prepared by adding 20-60ng / mL desmocyte growth factor and 15-25ng / mL bone formation protein in HCM medium. The culture medium is of positive components and high use safety; the revulsion is of short periodic time, high differentiation rate and stable safety, and has a wide application foreground.

Description

technical field

[0001] The invention relates to a method for inducing differentiation of human embryonic stem cells and a special medium thereof, in particular to a method for inducing differentiation of human embryonic stem cells to liver cells and a special medium for the same. Background technique

[0002] Stem cells are a type of cells with self-renewal ability and multi-lineage differentiation potential. Such cells can maintain the size or expansion of their own cell population through cell division, and can further differentiate into more mature cell types. According to the differentiation potential of stem cells, they can be divided into totipotent stem cells (such as embryonic stem cells, which can differentiate into all adult tissue cells, and even develop into complete individuals), pluripotent stem cells (with multi-directional differentiation potential, which can differentiate into cells other than Other types of tissue cells other than own tissue cells, such as...

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