Cislanche salsa determination leader DNA sequence and method therefor

A technology of Cistanche tubulosa and DNA sequence, which is applied in the field of identification of primer DNA sequence and identification, and can solve the problems of difficult and accurate identification of medicinal materials

Inactive Publication Date: 2007-01-17
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

However, traditional morphological identification requires a certain amount of experience, and it is even more difficult to accurately identify broken medicinal materials.

Method used

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  • Cislanche salsa determination leader DNA sequence and method therefor

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Embodiment Construction

[0041] The following examples further illustrate the present invention:

[0042] First, use the plant DNA extraction kit (QIAGEN, Germany) to extract total DNA from the original plants of different species of Cistanche medicinal materials, as well as the confusing products of Cynomorium, Cynomorium and other plants, and use PCR to amplify multiple gene fragments They were purified separately, and the purified fragments were subjected to DNA sequence analysis to establish a DNA sequence database of Cistanche Tubula medicinal materials and their fake and mixed products. On the basis of comparing databases, select appropriate DNA segments for the occurrence of fake or mixed medicinal materials, and design a pair of discriminatory PCR primers:

[0043] Primer 1: 5’-AGT CTT GAC CCG CGA TTT-3’;

[0044] Primer 2: 5'-ACA ACG GAA GGC ATA GAA-3'.

[0045] Use an automatic DNA synthesizer to synthesize the above-mentioned DNA sequence to obtain white powder crystals of the identification pr...

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Abstract

The present invention belongs to the identification technology field of Chinese traditional medicinal crops and discloses the identification primer sequences and the identification process of Cistanche tubulosa. The identification primer sequences of Cistanche tubulosa for polymerase chain reaction designed in this invention are: Primer1: 5'-AGT CTT GAC CCG CGATTT-3'; Primer2: 5'-ACA ACG GAAGGC ATA GAA-3'. The identification process of Cistanche tubulosa includes the isolation of DNA in the medicinal material and the detection of PCR product. The appearance of positive amplification bands in the result means that the tested sample is real Cistanche tubulosa while the negative result without the appearance of amplified bands means the tested sample is spurious. The present invention solves the identification problem of Cistanche tubulosa and is necessary for the production and marketing departments to identify Cistanche tubulosa and control the quality of medicinal materials.

Description

Technical field [0001] The invention is an identification primer DNA sequence and an identification method in the technical field of detecting Chinese medicinal materials, in particular to a Cistanche tubulosa identification primer DNA sequence and a Cistanche tubulosa identification method. Background technique [0002] Cistanche (Herba Cistanches), commonly known as Dayun. The Chinese Pharmacopoeia (2005 edition) stipulates that Cistanche is the dry fleshy stem with scale leaves of Orobanchaceae Cistanche deserticola YCMa or Cistanche deserticola YCMa or C.tubulosa (Schrenk) Wight. Intestinal laxative effect. Cistanche is a parasitic plant that is born in arid deserts. The host is Haloxylon ammodena (CAMey.) Bunge, H. persicum, Tamarixspp., Kalidium spp., etc. There are about 20 species in the world. In my country, there are C.deserticola, C.tubulosa, C.salsa, C.sinensis and Lanzhou Cistanche (C. .lanzhouensis), there are also reports that Lanzhou Cistanche is actually Cistanch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 李晓波王敬屠鹏飞
Owner SHANGHAI JIAO TONG UNIV
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