Modified skin tinea fungus test culture medium

Abstract

The modified dermatophyte test culture medium is compounded with soybean peptone 5g / L, glucose 5 g / L, chloromycetin 0.0625-0.25g / L, cycloheximide 0.1-0.5g / L, bromthymol blue 0.025-0.1g / L, agar 20g / L, and 1N HCl 4.8mL, and has pH value of 5.5 + / - 0.1. The present invention has the advantages of capacity of resulting in accurate interpretation, short culture period and low cost, except the advantages of traditional DTM culture medium. The present invention has latent clinical application foreground.

Description

technical field [0001] The invention relates to a fungal chromogenic medium, in particular to an improved dermatophyte test medium capable of rapidly distinguishing dermatophyte from non-dermatophyte fungi. Background technique [0002] Dermatophytes are a large group of fungi that can cause infections of the skin, hair, and nail plate in humans and animals. The incidence of dermatophytosis is very high, and the total number of patients in my country is estimated to be as high as hundreds of millions. Before clinical treatment, doctors often have to conduct fungal tests for patients. Only when it is clear that the infection is fungal and what kind of fungal infection is it, can effective antifungal drugs be selected for thorough treatment. Fungal culture is the "gold standard" for diagnosis. One of the key factors affecting the speed and accuracy of fungal culture is the culture medium. [0003] nitrogen source carbon source antibiotics Cycloheximide ...

AI Technical Summary

Problems solved by technology

[0006] DTM medium has high practical value because: 1. In recent years, the incidence of fungal infection has been increasing year by year, and the diagnosis and treatment of such diseases is the focus of attention of both government health departments and doctors and patients; 2. Different antifungal drugs The antimicrobial spectrum is not the same, some fungal infectious diseases such as onychomycosis have a long course of treatment and high cost, and the drugs have potential adverse reactions, so the identification of bacterial species and targeted and reasonable treatment are very important for such diseases It is especially important; 3. The traditional fungal culture identification method has high requirements on the experience and skills of the inspectors. At present, only a few hospitals in China have carried out this project, and it generally takes about 2 weeks to culture, and DTM medium produces positive results. In less than a week, the results are intuitive and can be reported by non-professionals

2. The result judgment standard needs to be improved

3. The antibiotic (chlortetracycline) in the medium is not resistant to high temperature and needs to be added after autoclaving, which is relatively cumbersome