Method of selecting and breeding wheat seed capable of resisting powdery mildow stripe rust and yellow dwarf disease

A technology for powdery mildew resistance and powdery mildew resistance genes is applied in the field of selective breeding of polygenes and powdery mildew resistance, and can solve the problems of high conditions and experience in the identification and identification of pathogenic races.

Inactive Publication Date: 2007-01-31
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the conditions and experience required for identification of pathogens by race inoculation are quite high, and in the early stage of isolation generation, it is also limited by the difficulty of inoculation and identification of the same single plant with multiple races at the same time.

Method used

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  • Method of selecting and breeding wheat seed capable of resisting powdery mildow stripe rust and yellow dwarf disease
  • Method of selecting and breeding wheat seed capable of resisting powdery mildow stripe rust and yellow dwarf disease
  • Method of selecting and breeding wheat seed capable of resisting powdery mildow stripe rust and yellow dwarf disease

Examples

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Embodiment 1

[0022] Example 1. Using molecular markers of 5 disease-resistant genes, including Pm4a-STS, Pm13-SCAR, Pm21-SCAR, Bdv2-SCAR and YrX-SSR, rapid and assisted breeding and resistance to powdery mildew, stripe rust and yellow dwarf Diseased wheat germplasm.

[0023] 1. Acquisition of test materials

[0024] 1. Field spring wheat germplasm 04P161~04P170 (BC 2 f 2 ) and its inbred line BC 2 f 3 get

[0025] 04P161~04P170 is 3×M269 / GP22-15, BC 2 f 2 The material is a spring yellow dwarf resistant wheat line M269 as the female parent, and GP22-15 (carrying Pm4a, Pm13, PmV, YrX) resistant to powdery mildew and stripe rust as the male parent to obtain F 1 , and then take M269 as the male parent and F 1 Backcross to get BC 1 Generation, with M269 (carrying Bdv2) as the male parent and BC 1 Backcross to get BC 2 Progeny plants, BC can be obtained after selfing once 2 f 2 Material 04P161~04P170, self-crossed again to get BC 2 f 3 Material. The self-bred plants of 04P161 wer...

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Abstract

The present invention discloses a method for breeding multigene polymerized wheat germplasm capable of resisting powdery mildew, stripe rust and verticillium wilt. Said method includes the following steps: utilizing STS marker of Pm4 to detect that the wheat to be detected has the gene Pm4a for resisting powdery mildew or not; utilizing SCAR marker of Pm13 to detect that the wheat to be detected has the gene Pm13 capable of resisting powdery mildew or not; utilizing SCAR marker of Pm21 to detect that the wheat to be detected has the gene PmV capable of resisting powdery mildew or not; utilizing SCAR marker of Bdv2 to detect that the wheat to be detected has the gene Bdv2 for resisting verticillium wilt or not; and utilizing SSR marker of YrX to detect that the wheat to be detected has the gene YrX for resisting stripe rust or not. Said invention provides a new method for breeding new wheat variety capable of resisting powdery mildew, stripe rust and verticillium wilt.

Description

technical field [0001] The invention relates to a method for breeding wheat germplasm with polygenetic resistance to powdery mildew, stripe rust and yellow dwarf disease. Background technique [0002] In recent years, due to changes in farming systems, fertilizer and water conditions, and climatic conditions, as well as the single source of resistance of wheat varieties, a wheat area is often infested by more than two kinds of pests and diseases. Powdery mildew and stripe rust have become the main diseases of stable and high yield of wheat in my country. Due to the serious occurrence of wheat aphids that spread its pathogen (barley yellow dwarf virus), yellow dwarf disease has a tendency to spread from major areas such as Gansu, Shanxi, and Shaanxi to the Jianghuai and southwest wheat regions. Therefore, the creation of new wheat germplasm resistant to powdery mildew, stripe rust and yellow dwarf disease is of great significance for the stable and high yield and sustainable...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 张增艳辛志勇陈孝林志珊曾祥艳徐惠君杜丽璞
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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