Method of selecting and breeding wheat seed capable of resisting powdery mildow stripe rust and yellow dwarf disease
A technology for powdery mildew resistance and powdery mildew resistance genes is applied in the field of selective breeding of polygenes and powdery mildew resistance, and can solve the problems of high conditions and experience in the identification and identification of pathogenic races.
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[0022] Example 1. Using molecular markers of 5 disease-resistant genes, including Pm4a-STS, Pm13-SCAR, Pm21-SCAR, Bdv2-SCAR and YrX-SSR, rapid and assisted breeding and resistance to powdery mildew, stripe rust and yellow dwarf Diseased wheat germplasm.
[0023] 1. Acquisition of test materials
[0024] 1. Field spring wheat germplasm 04P161~04P170 (BC 2 f 2 ) and its inbred line BC 2 f 3 get
[0025] 04P161~04P170 is 3×M269 / GP22-15, BC 2 f 2 The material is a spring yellow dwarf resistant wheat line M269 as the female parent, and GP22-15 (carrying Pm4a, Pm13, PmV, YrX) resistant to powdery mildew and stripe rust as the male parent to obtain F 1 , and then take M269 as the male parent and F 1 Backcross to get BC 1 Generation, with M269 (carrying Bdv2) as the male parent and BC 1 Backcross to get BC 2 Progeny plants, BC can be obtained after selfing once 2 f 2 Material 04P161~04P170, self-crossed again to get BC 2 f 3 Material. The self-bred plants of 04P161 wer...
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