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Albino tea tree breed 'sajin' specific DNA molecular mark and its identification method

A DNA molecule and identification method technology, applied in the direction of recombinant DNA technology, biochemical equipment and methods, DNA / RNA fragments, etc., can solve problems such as shortage of seedlings, not the highest season, changes in chemical composition content, etc.

Inactive Publication Date: 2007-05-02
ZHEJIANG UNIV
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0005] As the light-induced albino tea tree variety "Sajin" is a rare variety, there is a shortage of seedlings in the market
Moreover, this variety only shows albinism in high temperature and strong light seasons, and it is difficult to distinguish it from ordinary tea varieties in terms of external morphological characteristics in winter, the season when tea trees are suitable for planting.
Determination of chemical composition can also be used for identification of albino tea tree varieties, but the chemical composition content of tea trees varies with growing seasons and environmental conditions. During the planting period of winter tea trees, the characteristic compounds of albino tea trees, such as theanine content, are not the highest season , which is comparable to general tea tree varieties, so it is limited by seasons in specific applications

Method used

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  • Albino tea tree breed 'sajin' specific DNA molecular mark and its identification method

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Embodiment

[0028] 1 Test variety: In order to verify that the specific DNA molecular marker of the present invention is unique to the light-induced albino tea variety "Sajin", this example uses the common tea variety "Fuding Dabaicha" (No. 1 variety), induced by low temperature Type albino tea tree varieties "Baiyecha No. 1" (No. 2 variety), "Millennium Snow" (No. 3 variety), 2 light-induced albino tea varieties-"Golden Bud" (No. 4 variety) and Five varieties including "Sajin" (No. 5 variety) are the test materials. One bud, one leaf and one new shoot for each variety were used for identification.

[0029] 2 Genomic NDA extraction: weigh 100 mg of fresh leaves of the above tea tree species, put them into a mortar and 100 ml of liquid nitrogen, and quickly grind; quickly transfer the ground powder to a 1.5 ml centrifuge tube. Add 500μl of 2×CTAB preheated at 65°C and mix well, and in a water bath at 65°C for 5 minutes. Add an equal amount of chloroform-isoamyl alcohol (24:1) mixture, mix well...

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Abstract

The invention belongs to the field of biotechnology, involving biotechnology identification of tea varieties. It relates to the specific DNA molecular markers and identification of a light-induced albino tea varieties 'throwing', the molecular Weight of this nucleotide sequence marked by this specific DNA molecule is 285bp.. Its identification methods : from tea leaves to extract DNA, from the DNA by PCR amplification; PCR amplification products of electrophoresis, bands, 450-500bp emerging molecular weight of the bands detected tea is judged to 'throwing' varieties. Recycling molecular weight of this 450-500bp electrophoresis band plastic, amplification and sequencing; use the 285bp size public nucleotide sequence to check sequencing determine and found 'throwing' variety.

Description

Technical field [0001] The invention belongs to the field of biotechnology and relates to the biotechnological identification of tea tree varieties, specifically a light-induced albino tea variety "Sajin" specific DNA molecular marker and a method for identifying the variety "Sajin", and also belongs to the field of tea genetic breeding Application Technology. technical background [0002] The production of famous and high-quality tea has now become the main source of economic benefits for my country's tea industry. According to statistics, the output of my country's famous and high-quality tea accounts for about 30% of the total output, but the output value has reached about 70% of the total output value; Zhejiang Province, the main producing area of ​​famous and high-quality tea, even reaches 90%. The production of famous and high-quality tea depends on the promotion and processing technology of excellent varieties of tea trees, among which the varieties play a decisive role. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11C12P19/34
Inventor 梁月荣杜颖颖金晶陆建良林晨
Owner ZHEJIANG UNIV