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A method for determining brown strip resistant rice species

A leaf blight resistance and rice technology, applied in the field of identification of rice varieties resistant to stripe leaf blight, can solve the problems of identifying dead seedlings, increasing the workload of disease-resistant variety breeding, delaying disease-resistant breeding, etc. Achieve the effect of reducing workload, speeding up the process and improving accuracy

Inactive Publication Date: 2007-05-16
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology

Although there are many identification methods used in the process of disease-resistant breeding, most of them use the method of inoculation identification at the seedling stage. Due to the particularity of the damage mode of rice stripe virus, the results often lead to the death of identified plants with poor resistance. Therefore, breeders often infer the resistance of contemporary materials by identifying the resistance of the second generation, which not only delays the work process of disease-resistant breeding, but also increases the workload of disease-resistant variety breeding

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  • A method for determining brown strip resistant rice species

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Embodiment 1

[0020] Example 1, Inoculation Identification of Wuyujing No. 3 and Zhendao 88

[0021] Test inoculum:

[0022] According to the method shown in Fig. 1, the Q population of the rice stripe virus high-affinity SBPH population was screened, and the virus-carrying rate was 73% as detected by the RSV dot immunoassay.

[0023] Test plant material:

[0024] The rice stripe blight susceptible variety Wuyujing No. 3 was provided by the Wujin Rice and Wheat Breeding Farm in Jiangsu Province, and the rice stripe blight resistant variety Zhendao 88 was provided by the Hilly Agricultural Science Institute of the Jiangsu Academy of Agricultural Sciences. kind.

[0025] The resistance identification method is as follows:

[0026] 1. Culture and preparation of RSV inoculum

[0027] The Q population of SBPH is cultivated in a light-temperature culture room, the temperature is controlled at 15-25°C, and it is reared according to the method shown in Figure 2. When SBPH grows to 3-5 years old...

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Abstract

The invention relates to a method for checking the resistance of rice specy toward to leaf blight, wherein it based on the character of tilering plant to remove the tiler, to check the resistance at controllable condition; and based on the resistance appearance of tiler, evaluates the resistance of whole plant. The invention has high accuracy and wide check time.

Description

[0001] The invention is a new method for identifying the resistance of rice varieties to rice stripe blight, which belongs to the identification technology of plant disease resistance. Background technique [0002] Rice stripe blight caused by rice stripe virus (RSV) was first discovered in Kanto, Japan in 1897, and later occurred in Korea, Ukraine and China. Since the virus was discovered in Jiangsu and Zhejiang in 1962 in my country, it has occurred in 16 provinces, municipalities, and autonomous regions across the country. At present, the rice fields in the lower reaches of the Yangtze River, mainly in Jiangsu Province, are still in the epidemic period. According to incomplete statistics, the occurrence area of ​​rice stripe blight in Jiangsu Province reached 27 million mu in 2004 alone, accounting for 79% of the rice area in Jiangsu Province. Experts predict that rice stripe blight will still show a tendency to occur more heavily in the five years after 2006 , prevention w...

Claims

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Application Information

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IPC IPC(8): A01G16/00A01G7/00A01G22/22
Inventor 周彤周益军程兆榜
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES