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Method for preparing immobilized thallus and its use

A technology for bacterial cells and bacterial suspensions, applied in biochemical equipment and methods, chemical instruments and methods, microorganism-based methods, etc., can solve the problems of high immobilization cost, complex process, reduced bacterial activity, etc., and achieve low prices. , the effect of rich source of raw materials and high porosity

Inactive Publication Date: 2007-06-13
NANJING UNIV OF SCI & TECH
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Problems solved by technology

Usually, the immobilization of such materials will reduce the activity of the strain by 20-30%, and the cost of immobilization is high and the process is complicated.

Method used

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  • Method for preparing immobilized thallus and its use

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Embodiment Construction

[0017] The present invention will be described in further detail below in conjunction with the accompanying drawings.

[0018] In conjunction with the accompanying drawings, the preparation method of the immobilized thalline of the present invention comprises the following steps:

[0019] (1) Preparation of loofah sponge body: after drying the mature loofah gourd, removing the skin part, the loofah sponge body can be obtained. Then cut the sponge fiber into a disc with a diameter of about 2.5cm, 2-3mm thick, boil in boiling water for 30 minutes, rinse with tap water, soak in distilled water for 24 hours, and change the distilled water 3-4 times. The fully soaked sponge discs were dried at 70°C and stored in a desiccator.

[0020] (2) Slant culture: inoculate Trichoderma viride on the slant medium, culture at 28-30° C. for 3-5 days, and wait for spore formation for later use. Among them, the slant medium is a potato medium, and the potato medium is prepared by a conventional ...

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Abstract

This invention discloses a method to produce immobilized cells. The steps are as follows: Preparation of Spongiform vines, that is vines Spongiform fiber is got after the skin of dried mature vines is removed, then cut it into disk, put into boiling water and wash with water, immerse in distilled water, then dry the full immersion of the cavernous disc; Preparation of spores, inoculate the Trichoderma into the slant medium, cultivate at 20-40 degree C for 3-5 days, the spores are prepared for reservation; Preparation of the bacterial suspension, the spore suspension is prepared using the normal saline; Immobilization of the cells, inoculate spore suspension into medium and the Spongiform disk which are conducive to germination of mycelia in conical flask, immobilized cells are gained after cultivation at 20-30 degree C for 2-3 days at 100-200rpm. The advantages of the invention: high porosity of Spongiform vines, not dense entanglement generated about the immobilized mycelium, efficient adsorption of heavy metals in wastewater; Spongiform vines are natural porous fibers, don't contain harmful substances, and don't cause secondary pollution to water.

Description

a technical field [0001] The invention relates to heavy metal wastewater bioremediation technology, in particular to a preparation method and application of immobilized bacteria. Two background technology [0002] There are many traditional physical and chemical methods to deal with heavy metals, such as chemical precipitation, ion exchange, electrolysis, reverse osmosis, extraction, activated carbon adsorption, membrane separation, etc. They have their own advantages, but to varying degrees, there are disadvantages such as large investment, high energy consumption, difficult operation, and easy to produce secondary pollution. Especially when dealing with low-level heavy metal pollution, the operating cost and raw material cost are relatively high. With the development of biotechnology, microbial removal of heavy metal wastewater has received more and more attention since the 1980s. Biosorbents have attracted more and more attention due to their advantages such as simple fa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/16C12N1/14C02F3/34C02F1/62C12R1/885
CPCY02W10/37
Inventor 杨树林沈薇宁长发高力虎孟广荣李新柱梁广陆晓曾亮亮
Owner NANJING UNIV OF SCI & TECH
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