Production of glutamic acid based on pII feedback supplement
A feedback feeding and glutamic acid technology, which is applied in the field of adding organic carbon sources to produce glutamic acid based on pH feedback control, can solve the problems of low fermentation yield and affecting production control, and achieve simple operation, increased yield, and accurate Effect of Controlling Glucose Concentration
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Embodiment 1
[0020] The microbial strain used is Corynebacterium pekinense As1.299. The above strains were used for pH feedback controlled fed-batch fermentation, and there were two kinds of medium used:
[0021] 1) Seed medium: glucose 25 g / L, corn steep liquor 25 g / L, dipotassium hydrogen phosphate 1 g / L, magnesium sulfate 0.6 g / L, urea 5 g / L, Fe 2+ / Mn 2+ 2ppm, adjust the pH value to 6.7 with NaOH and hydrochloric acid, and sterilize at 115°C.
[0022] 2) Fermentation medium: glucose 120 g / L, corn steep liquor 6 g / L, dipotassium hydrogen phosphate 1 g / L, magnesium sulfate 0.6 g / L, Fe 2+ / Mn 2+ 2ppm, adjust the pH value to 7.0 with NaOH and hydrochloric acid, and sterilize at 115°C.
[0023] Take 50ml of seed culture medium and place it in a 1L shaker flask, insert fresh slanted seeds, cultivate on a shaker at 32°C and 260rpm for 8h, and insert 5% of the inoculum into a 5-liter automatic control fermenter containing fermentation medium. Control at: 0~10h, 32°C; after 10h, 36°C. In ...
Embodiment 2
[0025] Other fermentation conditions are with embodiment 1. When the concentration of residual sugar in the fermented liquid drops to 10 grams per liter (14h), start to feed the mixed solution of glucose and ammonia in the fermented liquid to control the pH and sugar concentration of the fermented liquid, and the glucose concentration in the mixed solution added is 500 grams / L, the ammonia concentration is 68.3 g / L. The control process is: when the pH drops to 6.95, the mixed solution of glucose and ammonia starts to flow automatically until the pH of the fermentation broth rises to 7.05. After the fermentation, the maximum output of glutamic acid was 53 g / L, and the conversion rate to glucose was 32.3%. During the fermentation process, the residual sugar concentration was maintained at 8.5-10.5 g / L. Compared with the glutamic acid yield (32.8 g / L, initial sugar concentration of 150 g / L) of batch fermentation without feeding, the yield increased by 0.62 times and the conver...
Embodiment 3
[0027]Other fermentation conditions are with embodiment 1. Fermentation was performed with pure biotin (2.84 μg / L) and thiamine (75.73 g / L) instead of corn steep liquor in the fermentation medium. When the concentration of residual sugar in the fermented liquid drops to 20 grams per liter (13h), start to feed the mixed solution of glucose and ammonia in the fermented liquid to control the pH and sugar concentration of the fermented liquid, and the glucose concentration in the mixed solution added is 500 grams / L, the ammonia concentration is 68.3 g / L. The control process is: when the pH drops to 6.95, the mixed solution of glucose and ammonia starts to flow automatically until the pH of the fermentation broth rises to 7.05. After the fermentation, the maximum output of glutamic acid was 48 g / L, and the conversion rate to glucose was 29.6%. During the fermentation process, the residual sugar concentration was maintained at 20-24 g / L. Compared with the glutamic acid yield (32...
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