Polynucleotide molecules encoding Neospora proteins

Inactive Publication Date: 2003-09-25
PFIZER INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Neospora caninum infects dogs, and congenitally infects pups, often leading to paralysis.

Method used

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Experimental program
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Embodiment Construction

[0026] 4.1. Polynucleotide Molecules

[0027] An isolated polynucleotide molecule of the present invention can have a nucleotide sequence derived from any species or strain of Neospora, but is preferably from a pathogenic species of Neospora such as N. caninum. A non-limiting example of a strain of N. caninum from which a polynucleotide molecule of the present invention can be isolated or derived is strain NC-1, which is available in host MARC-145 monkey kidney cells under Accession No. CRL-12231 from the American Type Culture Collection (ATCC), located at 10801 University Blvd, Manassas, Va., 20110, USA. Strain NC-1 is also described in Dubey et al., 1988, J. Am. Vet. Med. Assoc. 193:1259-63, which publication is incorporated herein by reference. Alternatively, pathogenic strains or species of Neospora for use in practicing the present invention can be isolated from organs, tissues or body fluids of infected animals using standard isolation techniques such as those described in the pu...

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Abstract

The present invention provides isolated polynucleotide molecules comprising nucleotide sequences encoding GRA1, GRA2, SAG1, MIC1 and MAG1 proteins from Neospora caninum, as well as recombinant vectors, transformed host cells, and recombinantly-expressed proteins. The present invention further provides a polynucleotide molecule comprising the nucleotide sequence of the bidirectional GRA1 / MAG1 promoter of N. caninum. The present invention further provides genetic constructs based on the polynucleotide molecules of the present invention that are useful in preparing modified strains of Neospora cells for use in vaccines against neosporosis.

Description

1. FIELD OF THE INVENTION[0001] The present invention is in the field of animal health, and is directed to vaccine compositions and diagnostics for disease. More particularly, the present invention relates to polynucleotide molecules comprising nucleotide sequences encoding GRA1, GRA2, SAG1, MIC1, and MAG1 proteins from Neospora, which polynucleotide molecules and proteins are useful in the production of vaccines against neosporosis, and as diagnostic reagents.2. BACKGROUND OF THE INVENTION[0002] Neospora is a pathogenic protozoan parasite of animals that has been recognized as a major cause of abortion, neonatal death, congenital infection, and encephalitic disease in mammals. Dubey and Lindsay, 1996, Vet. Parasitol. 67:1-59; Dubey and Lindsay, 1993, Parasitology Today, 9:452458. Neospora caninum infects dogs, and congenitally infects pups, often leading to paralysis. Tachyzoites of N. caninum have been isolated from naturally infected pups. Lindsay and Dubey, 1989, J. Parasitol. 7...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N15/02A61K31/00A61K35/12A61K38/00A61K39/00A61K39/002A61K39/02A61K39/07A61K39/08A61K39/085A61K39/09A61K39/106A61K39/108A61K39/112A61K39/12A61K39/145A61K39/15A61K39/205A61K39/215A61K39/245A61K39/295A61K39/39A61K48/00A61P15/06A61P25/00A61P31/00A61P31/04A61P31/12A61P33/00C07K14/44C07K16/18C07K16/20C12N1/11C12N1/15C12N1/19C12N1/21C12N5/10C12N15/09C12N15/30C12P21/02C12P21/08C12R1/90
CPCA61K38/00C07K14/44A61K39/00A61P15/06A61P25/00A61P31/00A61P31/04A61P31/12A61P33/00A61P33/02
Inventor KRISHNAN, B. RAJENDRAMADURA, REBECCAYODER, CHRISTINEDURTSCHI, BECKYBRAKE, DAVID A.
Owner PFIZER INC
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