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High-affinity antagonists of ELR-CXC chemokines

a chemokine and high-affinity technology, applied in the field of cxc chemokine receptor antagonists, can solve the problem that no cxc chemokine antagonists are known in the prior art that are effectiv

Inactive Publication Date: 2007-01-25
UNIVERSITY OF SASKATCHEWAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0007] Compositions of the present invention include novel ELR-CXC chemokine agonist and antagonist proteins that are capable of binding to CXCR1 or CXCR2 receptors in mammalian cells. These include agonists and antagonists that are capable of high-affinity binding, wherein “high-affinity” refers to the agonist's or antagonist's affinity for the receptor being sufficient such that it can block the wild-type chemokine agonist under physiologically relevant concentrations. The novel antagonist proteins also include those that are substantially equivalent (th

Problems solved by technology

But, despite active research in the field, no CXC chemokine antagonists are known in the prior art that are effective in suppressing adverse inflammatory cell activity induced by either ELR-CXC chemokine receptor.

Method used

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  • High-affinity antagonists of ELR-CXC chemokines
  • High-affinity antagonists of ELR-CXC chemokines
  • High-affinity antagonists of ELR-CXC chemokines

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Embodiment Construction

[0069] (The following abbreviations are used throughout this disclosure: ARDS, acute respiratory distress syndrome; BALF, bronchoalveolar lavage fluid(s); BHR, Bolton-Hunter Reagent; CXCR1, CXCR2, CXCL8 receptors A, B, respectively; ELR, glutamic acid-lysine-arginine motif; CXCL1, growth-related oncogenealpha; CXCL4, platelet factor-4; CXCL5, epithelial-derived neutrophil activator-78; CXCL6, granulocyte chemotactic protein-2; CXCL8, interleukin-8; fMLP, formyl methionyl-leucylproline bacterial tripeptide; IPTG, isopropyl-thio-D-galactopyranoside; MIP-2, macrophage inflammatory protein-2; PMSF, phenylmethylsulfonyl fluoride; TMB, tetramethylbenzidine.)

[0070] As used herein, ‘an agonist’ refers to an agent that causes a cell to become activated.

[0071] As used herein, ‘an antagonist’ refers to an agent that prevents the cell from being activated in the presence or absence of the agonist.

[0072] As used herein, “purified” does not require absolute purity but is instead intended as a ...

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Abstract

The present invention provides novel polypeptide sequences, methods for production thereof and uses thereof for novel ELR—CXC chemokine receptor agonists and antagonists.

Description

CROSS REFERENCE TO RELATED APPLICATION [0001] This application is a continuation-in-part of U.S. patent application Ser. No. 10 / 087,273, filed Mar. 1, 2002 which claims the benefit of U.S. Provisional Patent Application No. 60 / 273,181, filed on Mar. 1, 2001.FIELD OF THE INVENTION [0002] The present invention relates to the field of CXC chemokine receptor antagonists. BACKGROUND OF THE INVENTION [0003] The CXC chemokines that possess the receptor-signaling glutamic acid-leucine-arginine (ELR) motif (e.g., CXCL1 / GRO□, CXCL8 / IL-8; Baggiolini, M. 1998. Nature. 392:565-568) are important to the influx of inflammatory cells that mediates much of the pathology in multiple settings, including ischemia-reperfusion injury (Sekido, N. et al. 1993. Nature. 365:654-657; Villard, J. et al. 1995. Am. J. Respir. Crit. Care Med. 152:1549-1554), endotoxemia-induced acute respiratory distress syndrome (ARDS; Mukaida, N. et al. 1998. Inflamm. Res. 47 suppl. 3):S151-157), arthritis, and immune complex-t...

Claims

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Application Information

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IPC IPC(8): A61K38/19C07K14/52A61K38/00C12N15/09A61P9/10A61P11/00A61P13/12A61P15/14A61P43/00C07K1/22C07K14/47C07K14/54C07K19/00C12N1/15C12N1/19C12N1/21C12N5/10C12N7/00C12N15/12C12P21/02
CPCC07K14/5421A61P11/00A61P13/12A61P15/14A61P37/00A61P43/00A61P9/10
Inventor GORDON, JOHN R.LI, FANG
Owner UNIVERSITY OF SASKATCHEWAN
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