Liver tumor marker sequences

a marker sequence and liver tumor technology, applied in the field of liver tumor marker sequences, can solve the problems of lack of successful treatment options and no mutational model developed

Inactive Publication Date: 2007-02-22
FARNHAM PEGGY J +2
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

No mutational model has yet been developed for liver cancer as it has been for other cancers such as colon cancer.
With no clearly identified cause, successful treatment options are lacking.

Method used

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  • Liver tumor marker sequences
  • Liver tumor marker sequences
  • Liver tumor marker sequences

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Materials and Methods

[0039] Rapid Amplification of cDNA Ends (RACE). Rapid amplification of cDNA ends (RACE) was performed in both directions using the SMART cDNA amplification kit (Clontech) from mouse liver tumor polyA RNA. 5′ and 3′ RACE were performed using the gene-specific primers, GSP-A [5′-GCATGGCAAGAACAGACTGG-3′] (SEQ ID NO:5) and GSP-B [5′-GGATGAGAAGGGCATCTGGA-3′] (SEQ ID NO:6). 5′ and 3′ RACE products that were identified with the corresponding GSP primer were gel extracted and cloned into TOPO-TA vector (Invitrogen). Cloned products were sequenced by Big Dye (ABI) in the McArdle Laboratory Sequencing Facility (University of Wisconsin-Madison).

[0040] RNA Analysis. For analysis of murine CRG-L2 mRNA, total RNA was extracted from liver using guanidine thiocyanate / CsCl as described previously in (Lukas et al., 1999, incorporated by reference in its entirety). PolyA mRNA was isolated from 250 μg of total RNA using Oligotex mRNA Kit (Qiagen). RT-PCR was performed as describ...

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Abstract

Polypeptides whose expression is upregulated in liver tumor cells and cells from liver preneoplastic foci relative to expression in normal liver cells are disclosed as are polynucleotides that encode the polypeptides. In humans, the polynucleotide maps to a region of chromosome 15. The overexpression has also been confirmed in human liver, breast, colon and kidney cancer cell lines. It is believed that the polypeptides are overexpressed in tumor and preneoplastic cells in general.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS [0001] This application is a divisional application of U.S. application Ser. No. 10 / 620,532 filed on Jul. 16, 2003, which claims the benefit of U.S. provisional application Ser. No. 60 / 396,626, filed on Jul. 17, 2002.STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT [0002] This invention was made with U.S. Government Support from the following agency: NIH, Grant No. CA22484. The U.S. Government has certain rights in the invention.BACKGROUND OF THE INVENTION [0003] Primary liver cancer is the fifth most common cancer worldwide with approximately half a million cases reported in 1990. Hepatocellular carcinoma (HCC) accounts for 80% of all liver cancer and the rates of HCC have increased by over 70% in the last two decades in the U.S. The fatality ratio (mortality / incidence) of liver cancer is approximately 1, indicating that the majority of patients live less than a year. Late diagnosis due to lack of clinical symptoms is one of th...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12Q1/68G01N33/574C07H21/04C12P21/06C07K14/82C07K16/30A61K38/00C07K14/47
CPCA61K38/00C07K14/47C07K14/4748G01N33/57438
Inventor FARNHAM, PEGGY J.GRAVEEL, CARRIE R.HARKINS-PERRY, SARAH R.
Owner FARNHAM PEGGY J
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