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Method for Transdifferentiating Cells

a technology of transdifferentiating cells and cells, applied in the field of transdifferentiating cells, can solve the problems of difficult control of such cells, inability to control the differentiation direction, and not only the technology requires time and effort to obtain a target cell

Inactive Publication Date: 2007-12-27
KAZUHITO SATOMURA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0009] An object to be achieved by the present invention is to address the above-described problems of conventional technologies. Specifically, an object to be achieved by the present invention is to provide a method for transdifferentiating a mature cell without using a DNA methylating agent or a DNA demethylating agent.

Problems solved by technology

At the same time, such cells have many problems, such that control of such cells is difficult because of their pluripotency.
With this technology, currently, cell transdifferentiation can be caused to take place successfully by causing DNA methylation or DNA demethylation, but differentiation direction cannot be controlled.
Accordingly, the technology not only requires much time and effort to obtain a target cell, but also involves the risk that cells could undergo unpredicted mutation.
Thus, this technology is extremely risky in medical applications or industrial uses.
However, this technology is merely a technology that uses undifferentiated cells such as fetal cells or progenitor cells.

Method used

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  • Method for Transdifferentiating Cells
  • Method for Transdifferentiating Cells
  • Method for Transdifferentiating Cells

Examples

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Effect test

examples

[0053] (1) Method

[0054] Mouse osteoblasts of an established line (MC3T3 / E1) were cultured to subconfluency in an αMEM medium supplemented with FBS (final concentration: 10%), 5 mM to 10 mM β-glycerophosphate, 50 μg / ml ascorbic acid, and 1×Glutamax (hereinafter, the medium for osteoblasts).

[0055] Cells cultured in the medium for osteoblasts were washed with a DMEM medium. Subsequently, the culture conditions of the cultured MC3T3 / E1 were changed from culture using the medium for osteoblasts to culture using an AMEM medium supplemented with FBS (final concentration: 10%), 100 ng / ml bFGF, 10 ng / ml FGF-8, 10 ng / ml EGF, and 10 ng / ml BDNF (hereinafter, the medium for nerve cells). Changes in the cells caused by the change of the media were examined with time through morphological observation by microscopic examination and through the analysis of protein expression by immunostaining.

[0056] (2) Result

[0057] MC3T3 / E1 cells cultured in the medium for osteoblasts were transferred to the me...

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Abstract

An object of the present invention is to provide a method for transdifferentiating a mature cell without using a DNA methylating agent or a DNA demethylating agent. The present invention provides a method for transdifferentiating a finally differentiated mature cell having a first differentiation phenotype into a finally differentiated mature cell having a second differentiation phenotype that differs from the above first differentiation phenotype by changing culture conditions for the finally differentiated mature cell having the first differentiation phenotype, wherein culture conditions suitable for culturing the finally differentiated mature cell having the above first differentiation phenotype are changed to culture conditions suitable for culturing the finally differentiated mature cell having the above second differentiation phenotype.

Description

TECHNICAL FIELD [0001] The present invention relates to a method for transdifferentiating cells. More specifically, the present invention relates to a method for transdifferentiating cells by varying the environment for culturing finally differentiated mature cells. The present invention further relates to differentiated cells that can be obtained by the above method. BACKGROUND ART [0002] Securing of a source for supplying target cells is essential in any field, based on which regenerative medicine or cellular medicine is realized. Various techniques therefor, such as techniques using stem cells or mature cells, have been developed to date. [0003] Currently, a merit of pluripotent stem cells represented by embryonic stem cells (ES cells) and mesenchymal stem cells (MSCs) that are mainly used in the field is their pluripotency. At the same time, such cells have many problems, such that control of such cells is difficult because of their pluripotency. [0004] In the meantime, transdif...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N5/06C12N5/00C12N5/079
CPCC12N5/0018C12N5/0618C12N2501/11C12N2506/13C12N2501/119C12N2501/13C12N2501/115
Inventor SATOMURA, KAZUHITO
Owner KAZUHITO SATOMURA
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