Transport media

Active Publication Date: 2009-02-19
PRETIUM CANADA PACKAGING ULC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the presence of charcoal particles can interfere with the Gram interpretation of the specimen.

Method used

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Examples

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Embodiment Construction

[0006]To prepare transport media according to the invention, various ingredients are solubilized in water. In an exemplary embodiment, components as listed below are suspended in 1.0 liters of distilled water; heated to boiling with frequent agitation to solubilize the ingredients; dispensed into screw-capped tubes; and sterilized at 121° C. for 15 minutes.

Activated charcoal0.50 gramsMOPS Acid3.15 gramsMOPS Salt8.48 gramsPotassium Chloride1.00 gramsCalcium Chloride1.26 gramsMagnesium Chloride5.48 gramsSodium Thiosulfate10.90 grams N-Acetyl Glucosamine0.50 gramsBetaine5.00 gramsGluten Hydrolysate from maize0.50 gramsAgar7.50 grams

[0007]The media was tested in accordance with the procedures set forth in Quality Control of Microbiological Transport Systems; Approved Standard, CLSI, (formerly NCCLS) M-40A. Strains included Neisseria gonorrhoeae (NG), Haemophilus influenzae (HI), Streptococcus pneumoniae (SPN), Streptococcus pyogenes (SP), P. aeruginosa (PA) and S. aureus (SA) and E. col...

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Abstract

A transport media, particularly for fastidious microorganisms, has a concentration of activated charcoal less than 10 grams per litre of water. This maintains viability of the microorganisms while permitting Gram interpretation of the specimen.

Description

FIELD OF THE INVENTION[0001]The present invention relates to media for transporting microorganisms.BACKGROUND[0002]Maintaining the viability of clinically relevant pathogens for subsequent diagnosis is a major concern in the healthcare field, especially in outpatient settings where delay in transit may be expected, and specialized transport media is utilized for this purpose.[0003]The use of transport media was first popularized in the 1940's, when Robert Stuart, a public health microbiologist in Glasgow, Scotland, developed and disclosed to the world a transport medium consisting of sodium glycerophosphate, sodium thioglycollate, methylene blue, agar and calcium chloride. The Stuart media was subsequently improved by C. R. Amies, of the Ontario Public Health Laboratory, who substituted an inorganic phosphate buffer for the glycerophosphate of the Stuart media, and added sodium, calcium and magnesium salts. Both the Stuart and Amies media remain widely used for transporting a wide v...

Claims

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Application Information

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IPC IPC(8): C12N1/20
CPCC12N1/04
InventorFINN, SUSAN J.
OwnerPRETIUM CANADA PACKAGING ULC