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Sensor for detecting stem cell differentiation based on electrochemical methods

a technology of electrochemical methods and sensors, applied in the field of sensors for detecting stem cell differentiation, can solve the problems of not using electrical or electrochemical systems to detect the differentiation of mes cells, and the size of the optical system is limited

Inactive Publication Date: 2010-09-30
IND UNIV COOP FOUND SOGANG UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Optical systems are limited by size of the instrument and the process of transforming the optical signal into an electrical signal [1-2].
However, there has been no attempt to detect the differentiation of MES cells using electrical or electrochemical systems.

Method used

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  • Sensor for detecting stem cell differentiation based on electrochemical methods
  • Sensor for detecting stem cell differentiation based on electrochemical methods
  • Sensor for detecting stem cell differentiation based on electrochemical methods

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Experimental Materials and Methods

2.1 Materials

[0046]1-Naphthyl phosphate and phosphate buffered saline (PBS) (pH 7.4, 10 mM) solution was purchased from Sigma-Aldrich. All other chemicals that are used in this study were obtained commercially as reagent grade.

2.2 Undifferentiated MES Cell Culture and Embryonic Body Formation

[0047]J1 cells, mouse embryonic stem (ES) cells, were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 15% FBS, 1 mM sodium pyruvate, 10−4M 2-mercaptoethanol, 1×nonessential amino acids, and 1,000 U of leukemia inhibitory factor (LIF) per ml at 37° C. and 5% CO2. EB formation was performed with J1 ES cells as previously described [13]. ES cells (2×106 cells) were seeded onto the bacterial-grade Petri dishes in DMEM containing 10% FBS without LIF. After 2 days of suspension culture, EBs were collected and trypsinized. A total of 1.37×106 cells were replated on 0.2% gelatin-coated 6-well plates containing the same medium. The number of cells...

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Abstract

This invention relates to a sensor for detecting a stem cell differentiation, including (a) an electrode; and (b) a substrate of an alkaline phosphatase. The phosphorylation or dephosphorylation of the substrate for an alkaline phosphatase as a stem cell undifferentiation marker which dephosphorylates its substrate may be measured using an electrical signal in the present sensor. Therefore, the sensor of the present invention enables to electrically detect a stem cell status in a high-throughput manner and to determine the stem cell differentiation.

Description

CROSS-REFERENCE TO RELATED APPLICATION[0001]This application claims under 35 U.S.C. §119(a) the benefit of Korean Application No. 10-2009-0026353 filed Mar. 27, 2009, the entire contents of which are incorporated herein by reference.BACKGROUND[0002]1. Technical Field[0003]The present invention relates to a sensor for detecting a stem cell differentiation, including: (a) an electrode; and (b) a substrate for an alkaline phosphatase.[0004]2. Background Art[0005]Cell chip technology is a promising tool for utilization in cell based assays. There are two kinds of cell detection systems for these chips. These systems are based on optical detection and electrical (electrochemical) detection respectively. Optical systems allow one to observe visual changes of the cells and have high sensitivity and selectivity. Optical systems are limited by size of the instrument and the process of transforming the optical signal into an electrical signal [1-2].[0006]While electrical cell detecting system...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12Q1/42G01N33/50
CPCC12Q1/42C12Q1/005C12Q1/001C12Q1/04G01N27/26C12N5/0606
Inventor CHOI, JEONG-WOOYEA, CHEOL-HEON
Owner IND UNIV COOP FOUND SOGANG UNIV
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