Fertilized egg isolate and use thereof
a technology of fertilized egg and isolate, which is applied in the field of fertilized egg isolate, can solve the problems of devastating impact on social structure and societal economics, patients with depression are often impaired in function, and the antidepressant is only effective in a subset of depressed patients
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example 1
[0062]Fertilized hen eggs that were 8-9 days old were selected. The whole egg was disinfected with 70% ethanol and left in the fume hood to allow the 70% ethanol to evaporate. The eggs were broken and the contents were dropped through a sterile 1.0 mm mesh. The shells and filtrate were discarded. The retentate, both solid and liquid, was collected and chilled on ice. The retentate was homogenized at 5° C. The homogenate (slurry) was poured into sterile stainless steel trays, frozen, and freeze-dried. The dried product was pulverized with a grinder. Preservatives 0.5% w / w sodium benzoate and 0.2% w / w potassium sorbate were added to the powder, and the mixture was blended. The finished powder was stored at 2-8° C. (short term) or −20° C. (long term).
example 2
[0063]Chicken embryos were harvested at 8 days old from fertilized hen eggs according to the following method:
[0064](i) 24 brown eggs were received;
[0065](ii) when cracked open, only 22 of 24 eggs where found fertilized containing the developed embryo (91.6% fertilization rate);
[0066](iii) embryos were removed and washed (×2) briefly in sterile PBS and immediately frozen at −20° C. in individual 50 mL (milliliter) test tubes;
[0067](iv) 10 test tubes were then freeze dried in a freeze dryer (shelf temp.=−40° C., condenser temp. −52° C.);
[0068](v) at the end of the cycle, the individual embryos (while in test tube) were crushed with a sterile glass rod and the resulting powder (1.7 g (grams)) was packaged;
[0069](vi) the 12 remaining embryos were pooled in a small beaker and blended with a small amount of water using a hand held blender;
[0070](vii) the resulting slurry was immediately frozen and subsequently freeze dried as above.
[0071]Results
[0072]Once dried, the powder (1.7 g) was di...
example 3
[0074]Sixty fertilized eggs (8 day old embryos) were received. The following is a description of the preparation of both the aqueous and the ether isolates.
[0075]All eggs were sprayed with 70% (v / v) ethanol to sterilize the external surface and the eggs were left in the laminar flow hood for the alcohol to evaporate. The eggs were broken and the embryos and the attached clear sac with its clear watery content were carefully separated from the rest of yolk and vascular material. Then, all material, that is, the embryo and clear sacs, was pooled in a beaker that had been chilled on ice. Following the harvest, the material was blended with the help of a hand held blender, resulting in a slurry.
[0076]The resulting slurry (approx. 200 mL) was split into two equal halves; one was used for extraction with water and the second for ether extraction.
[0077]Aqueous Isolate
[0078]To the 100 mL slurry, 100 mL of R.O. water was added and the material was stirred at room temperature for 30 minutes. ...
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