Method For Producing Fractions of a Milk Composition
Treating milk with phospholipase and separating it into fractions with different fat content addresses the fouling issue in milk processing, enhancing butter yield and emulsification properties, and improving heat stability.
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example 1
[0067]Un-homogenized whole milk was used to produce three different samples of skim milk and two samples of cream by the following methods:
[0068]Sample 1.1
[0069]The milk was incubated at 40° C. for 30 minutes with a phospholipase from Fusarium venenatum. Enzyme dosage was 700 LEU / I milk. The enzyme reaction was terminated by heating the milk to 72° C. for 10 minutes. The milk was centrifuged at 3000 g for 20 minutes and separated in skim milk and cream.
[0070]Sample 1.2
[0071]The milk was held at 40° C. for 30 minutes without addition of phospholipase and heat treated at 72° C. for 10 minutes. The milk was centrifuged at 3000 g for 20 minutes and separated in skim milk and cream.
[0072]Sample 1.3
[0073]Skim milk from sample 1.2 was incubated at 40° C. for 30 minutes with a phospholipase from Fusarium venenatum. Enzyme dosage was 700 LEU / I milk. The enzyme reaction was terminated by heating the milk to 72° C. for 10 minutes
[0074]Surface tension of the three skim milk samples using a tens...
example 2
[0076]The two cream fractions obtained from sample 1.1 and sample 1.2 of experiment 1 were used for churning of butter in a Kitchen Aid whipping machine operated at maximum speed. 250 g cream was cooled to 5° C. before churning and the whipping / churning was performed at room temperature.
[0077]The butter and buttermilk was separated using a sieve and the amount of butter was measured by weighing. The cream separated from the enzyme treated whole milk of sample 1.1 gave a butter yield of 34.76% of the cream, whereas the cream from the untreated milk of sample 1.2 resulted in 33.32%.
example 3
[0078]The two skim milk fractions from the sample 1.1 and sample 1.2 of example 1 were used for production of acidified milk. 9 parts skim milk was incubated with 1 part commercial acidified buttermilk and incubated for 20 hours at room temperature. The incubation was made directly in the device used for viscosity measurements (Rapid Visco Analyzer RVA-4. Newport Scientific, Australia). This consists of a measure cylinder mounted with a spindle. Five sample of each treatment were prepared. The spindle was rotated at 100 rpm and the viscosity was recorded continuously during 15 minutes of rotation. The average viscosity was 362 cp (centipoise) for the enzyme treated acidified skim milk from sample 1.1 and 406 cp for the acidified skim milk not treated with enzyme from sample 1.2.
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