SAB as a Biomarker for Degenerative Diseases and Therapeutic Sensitivity in Cancers

Abstract

The current invention pertains to a method of diagnosing a disease or identifying an increased likelihood of developing the disease in a subject. The method comprises determining the level of Src homology 3 domain binding protein 5 (SH3BP5 or SAB) or the RNA encoding SAB protein in a biological sample obtained from the subject and identifying the subject as having the disease or having an increased likelihood of developing the disease if the biological sample obtained from the subject has an altered level of SAB protein or the RNA encoding SAB protein relative to a control sample. The methods of the current invention can be practiced to diagnose and treat a systemic degenerative disease, a neurodegenerative disease, obesity, diabetes, a cancer, or an aging related disease. The invention also provides a kit for diagnosing a disease or diagnosing an increased likelihood of developing the disease in a subject.

Description

CROSS REFERENCE TO RELATED APPLICATION(S)[0001]This application claims the benefit of U.S. Provisional Application Ser. No. 62 / 026,894, filed Jul. 21, 2014, which is hereby incorporated by reference in its entirety, including any figures, tables, or drawings.[0002]The Sequence Listing for this application is labeled “SEQ-LIST-07-21-15-ST25.txt”, which was created on Jul. 21, 2015, and is 2 KB. The entire content is incorporated herein by reference in its entirety.BACKGROUND OF INVENTION[0003]The current technology for diagnosing degenerative diseases focuses on blood factors that are commonly associated with normal immune responses. Since these blood factors are associated with normal immune responses, such biomarkers do not provide an accurate prediction of the occurrence of degenerative diseases. Therefore, there is a need for a more specific biomarker for diagnosis of degenerative diseases.[0004]The protein Src homology 3 binding protein 5 (SH3BP5 or SAB) is a scaffold protein fo...

AI Technical Summary

Benefits of technology

The subject invention provides materials and methods for monitoring and modulating mitochondrial function. Specifically, the invention may help detect and diagnose degenerative diseases and cancer cells that are susceptible to treatment with apoptosis-inducing chemotherapeutic agents and / or radiation. The invention is based on the discovery of a protein called Src homology 3 domain binding protein 5 (SH3BP5 or SAB) which coordinates signaling components on the outer mitochondrial membrane. SAB can help maintain the integrity of the mitochondrial membrane potential, and its degradation can lead to the recruitment of death-inducing proteins to the mitochondrial surface. The invention also includes assays for detecting and monitoring SAB protein levels.

Problems solved by technology

Specifically, these signaling components destabilize the integrity of the mitochondrial membrane potential thereby reducing metabolic efficiency, which can lead to the recruitment of death-inducing proteins to the mitochondrial surface.

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