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SAB as a Biomarker for Degenerative Diseases and Therapeutic Sensitivity in Cancers

a biomarker and cancer technology, applied in the field of blood factors, can solve the problems attracting death-inducing proteins to the mitochondrial surface, etc., and achieve the effects of reducing metabolic efficiency, monitoring and modulating mitochondrial function, and destabilizing integrity

Inactive Publication Date: 2016-01-21
FLORIDA INTERNATIONAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The subject invention provides materials and methods for monitoring and modulating mitochondrial function. Specifically, the invention may help detect and diagnose degenerative diseases and cancer cells that are susceptible to treatment with apoptosis-inducing chemotherapeutic agents and / or radiation. The invention is based on the discovery of a protein called Src homology 3 domain binding protein 5 (SH3BP5 or SAB) which coordinates signaling components on the outer mitochondrial membrane. SAB can help maintain the integrity of the mitochondrial membrane potential, and its degradation can lead to the recruitment of death-inducing proteins to the mitochondrial surface. The invention also includes assays for detecting and monitoring SAB protein levels.

Problems solved by technology

Specifically, these signaling components destabilize the integrity of the mitochondrial membrane potential thereby reducing metabolic efficiency, which can lead to the recruitment of death-inducing proteins to the mitochondrial surface.

Method used

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  • SAB as a Biomarker for Degenerative Diseases and Therapeutic Sensitivity in Cancers
  • SAB as a Biomarker for Degenerative Diseases and Therapeutic Sensitivity in Cancers
  • SAB as a Biomarker for Degenerative Diseases and Therapeutic Sensitivity in Cancers

Examples

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example 1

In-Cell Western Blot Analysis

[0148]Cells or tissue samples are placed in a fluorescent compatible plate (for example, a 96-well plate) and the sample can be fixed to the plate. The sample is then permeabilized using detergent to disrupt the plasma membrane. Primary antibodies to detect SAB (Abnova, Inc.) are then incubated with the samples. Following a brief wash, the cells are incubated with a fluorescently labeled secondary antibody used to recognize the primary antibody. The samples are washed again. The plate is then scanned on a Li-Cor Bioscience Odyssey CLx near-infrared scanner to measure the fluorescent signal from the sample. A high fluorescent signaling indicates a higher level of SAB protein, while a low fluorescence signal indicates a lower level of SAB protein.

[0149]This assay can be used to detect SAB protein concentrations in a variety of cells and tissues. The assay can be useful for adherent cells, non-adherent cell, and primary cells. The assay can be modified to d...

example 2

SAB Levels in Various Disease Conditions

[0150]Parkinson's disease-like state was induced in animals by localizing treatment to the ventral midbrains of rats using 6-hydroxydopamine. Blood was obtained from 6-hydroxydopamine treated rats and the control rats. Levels of SAB protein or RNA encoding SAB proteins in platelets and other blood cells were higher in 6-hydroxydopamine treated rats compared to control rats.

[0151]Similarly, blood samples can be obtained from humans suffering from Parkinson's disease and humans free from Parkinson's disease. Levels of SAB protein or RNA encoding SAB proteins in platelets and other blood cells will be higher in the humans suffering from Parkinson's disease compared to the humans free from Parkinson's disease.

[0152]Tissues samples from ventral midbrain and muscles were obtained from young rats and aged rats. SAB protein levels were determined in the two sets of samples. SAB levels were higher in the ventral midbrains and muscles of aged rats compa...

example 3

Correlation Between SAB Expression, Mitochondrial Priming, and Susceptibility of a Cancer to Apoptosis Inducing Chemotherapeutic Agents

[0155]Mitochondrial priming influences cancer cell responses to chemotherapy. Mitochondrial priming is the process potentiating mitochondrial physiology towards a pro-apoptotic response. In cancer cells, mitochondrial priming has been used as means to sensitize cells to chemotherapy and radiotherapy approaches. Mitochondrial priming drives cancer cells toward apoptosis by exploiting their unique biology and permits physicians to administer lower doses of toxic chemotherapies. The amount of mitochondrial priming can be used as a predictive index of chemotherapeutic success. Elevated levels of pro-apoptotic Bcl-2 family members are seen on primed mitochondria. Therefore, detection of primed mitochondria in tumors can be used as a biomarker for chemotherapeutic success (FIG. 10).

[0156]HeLa cell mitochondria were primed using a sub-chronic (2 μM dose) of...

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Abstract

The current invention pertains to a method of diagnosing a disease or identifying an increased likelihood of developing the disease in a subject. The method comprises determining the level of Src homology 3 domain binding protein 5 (SH3BP5 or SAB) or the RNA encoding SAB protein in a biological sample obtained from the subject and identifying the subject as having the disease or having an increased likelihood of developing the disease if the biological sample obtained from the subject has an altered level of SAB protein or the RNA encoding SAB protein relative to a control sample. The methods of the current invention can be practiced to diagnose and treat a systemic degenerative disease, a neurodegenerative disease, obesity, diabetes, a cancer, or an aging related disease. The invention also provides a kit for diagnosing a disease or diagnosing an increased likelihood of developing the disease in a subject.

Description

CROSS REFERENCE TO RELATED APPLICATION(S)[0001]This application claims the benefit of U.S. Provisional Application Ser. No. 62 / 026,894, filed Jul. 21, 2014, which is hereby incorporated by reference in its entirety, including any figures, tables, or drawings.[0002]The Sequence Listing for this application is labeled “SEQ-LIST-07-21-15-ST25.txt”, which was created on Jul. 21, 2015, and is 2 KB. The entire content is incorporated herein by reference in its entirety.BACKGROUND OF INVENTION[0003]The current technology for diagnosing degenerative diseases focuses on blood factors that are commonly associated with normal immune responses. Since these blood factors are associated with normal immune responses, such biomarkers do not provide an accurate prediction of the occurrence of degenerative diseases. Therefore, there is a need for a more specific biomarker for diagnosis of degenerative diseases.[0004]The protein Src homology 3 binding protein 5 (SH3BP5 or SAB) is a scaffold protein fo...

Claims

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Application Information

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IPC IPC(8): G01N33/68A61K31/167A61K31/519A61K31/5377A61K31/69A61K33/24A61K38/07A61K47/48C07K16/28C12N7/00C12N15/113C12Q1/68
CPCG01N33/6893C07K2317/24A61K31/5377A61K33/24C12N15/113C12N7/00A61K31/167A61K38/07A61K31/69A61K31/519C07K16/2887C07K16/2878A61K47/48384G01N2800/50G01N2800/52G01N2500/10C12Q2600/136C12Q2600/106C12Q2600/118C12N2710/10071C12N2310/122C12N2310/531C12N2710/10043C12N2710/10023C12Q1/6883C07K16/18C12N2750/14143C12Q1/6886C12Q2600/158G01N33/5079G01N33/574G01N33/6872
Inventor CHAMBERS, JEREMY, W.
Owner FLORIDA INTERNATIONAL UNIVERSITY
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