Sugar chain array

Abstract

A sugar chain array containing a sugar chain immobilized thereon for detecting binding between an analyte and a sugar chain, and a sugar chain array having the sugar chain immobilized thereon that is capable of inhibiting non-specific adsorption and binding of an analyte without having to coat the array with an adsorption inhibitor. A specific sugar chain is immobilized on the array, and is useful for detecting binding between the sugar chain and an analyte, such as a pathogen of an infectious disease or an excretion thereof. In addition, in the array, when a base material is coated with a polymeric compound having a unit having a primary amino group, a unit for maintaining hydrophilicity, and a unit having a hydrophobic group, the sugar chain is immobilized efficiently and non-specific adsorption and binding of the analyte can be effectively inhibited.

Description

TECHNICAL FIELD[0001]The present invention relates to a sugar chain array capable of detecting binding between an analyte and a sugar chain.BACKGROUND ART[0002]In the field of biochemistry, sugar chains have attracted attention in recent years as a third type of chain following nucleic acids and proteins. In particular, research has been conducted on their involvement with cell differentiation and malignant transformation, immune response and fertilization, and attempts are continuing towards the development of new pharmaceuticals and medical materials.[0003]In addition, sugar chains serve as receptors for numerous toxins, viruses and bacteria and are also attracting attention as tumor markers, and attempts are similarly continuing towards the development of new pharmaceuticals and medical materials in these fields as well.[0004]However, although the importance of research on sugar chains is recognized, due to their complex structure and diversity, the pace at which this research ha...

AI Technical Summary

Benefits of technology

[0036]Use of the sugar chain array of the present invention makes it possible to efficiently detect interaction between an analyte and a sugar chain. In particular, in this sugar chain array, by using a base material coated with a polymeric compound containing a unit having a primary amino group, a unit for maintaining hydrophilicity, and a unit having a hydrophobic group, the sugar chain can be immobilized efficiently and non-specific adsorption and binding of the analyte can be inhibited.

Problems solved by technology

However, although the importance of research on sugar chains is recognized, due to their complex structure and diversity, the pace at which this research has progressed is considerably slower in comparison with the first and second types of chains in the form of nucleic acids and proteins.

However, carrying out this blocking procedure following sugar chain immobilization is both complicated and troublesome.

However, in this method, it is necessary to introduce an acetyl halide group into the solid support when immobilizing the sugar chain on the base material, and although this is comparatively easy in the case the material of the base material is glass, this method lacks versatility with respect to other materials.

In this manner, in addition to delays in the development of suitable techniques and base materials for immobilizing sugar chains, development has also been delayed with respect to sugar chain arrays on which sugar chains are immobilized that are useful for analyzing interactions between analytes and sugar chains.