39 results about "Cell substance" patented technology

In vitro culture devices and methods are described. The subject methods and devices provide a means whereby cells and/or subcellular material are grown or held in a culture device that maintains the cells and/or subcellular material in a physiologically representative environment, thereby improving the predictive value of toxicity and metabolism assays, and the relevance of experimental results derived from such assays to actual in vivo conditions, processes and outcomes. The culture devices of the invention comprise a fluidic channel connected to or otherwise integrated with at least one chamber, preferably integrated in a chip format. The specific chamber geometry is designed to provide cellular interactions, liquid flow, and liquid residence and other parameter values that correlate with those found in or produced by the corresponding cell, organs or tissues, or components or products thereof, in vivo. Each device comprises at least one chamber and at least one inlet and one outlet port that allow for recirculation of the culture medium. The device will usually include a mechanism for obtaining signals from the cells and culture medium.

The invention provides a spray nozzle with double flow channels. The spray nozzle comprises a spray nozzle unit with the inner flow channel, a spray nozzle unit with the outer flow channel, a connector and a spray nozzle body. The spray nozzle unit with the inner flow channel and the spray nozzle unit with the outer flow channel are connected to the spray nozzle body by the connector, and a spinneret structure is formed in the spray nozzle body in such a manner that an inner-layer material squeezed out of the spray nozzle unit with the inner flow channel is positioned in the center of the spinneret structure and an outer-layer material squeezed out of the spray nozzle unit with the outer flow channel wraps the periphery of the spinneret structure. The spray nozzle with the double flow channels has the advantages that the two different materials are respectively arranged in the inner flow channel and the outer flow channel by the aid of inner and outer double-flow-channel technologies, accordingly, the spinneret structure with the corresponding material in the center and the other material uniformly arranged on the periphery can be formed by means of squeezing, the central material can be effectively protected, and cell substances in the center of the spray nozzle can be protected when the spray nozzle is applied to 3D (three-dimensional) biological printing; nutrients can be provided, so that the survival rate of printed biological cells can be increased.

The invention relates to a confocal-photoacoustic dual-mode microscopic imaging method which comprises the following steps of: (1) carrying out confocal microscopic imaging to an observed object and carrying out photoacoustic scanning imaging to the observed object; and (2) acquiring the functional image of biological tissue by comparing dual-mode images. The invention also relates to a confocal-photoacoustic dual-mode microscopic imaging device which comprises a confocal microscopic imaging system and a photoacoustic scanning imaging system, wherein the confocal microscopic imaging system mainly comprises a laser, a laser scanning galvanometer, a pinhole and a photomultiplier, and the photoacoustic scanning imaging system is formed by sequentially and electrically connecting a photoacoustic sensor, a chopper, a phase-locked amplifier, a data acquisition card and a computer. The invention can carry out confocal-photoacoustic dual-mode microscopic imaging to cells for acquiring the structure information, the content space distribution information and the function information of the cells and the dynamic distribution information of the cell substance contents.

The invention discloses a method for co-culturing, inducing and amplifying gamma delta T cells and NK cells. The gamma delta T cells and the NK cells are obtained in a co-culture manner; peripheral blood is collected to separate and screen mononuclear cells and stimulate and induce the mononuclear cells to induce the gamma delta T cells and the NK cells, the gamma delta T cells and the NK cells are subjected to activation amplification culture to amplify a gamma delta T cell and NK cell composite cell substance having characteristics of large number, high amplification multiple, strong cytotoxicity and the like so as to form the co-culture cell composition through compounding, and the co-culture cell composition has good clinical application value. The co-culture induced amplification method improves the cell culture efficiency, greatly reduces the culture cost, reduces the difficulty of a culture technology, simplifies the clinical treatment process, reduces the treatment cost, and simplifies the treatment means.

The invention belongs to the field of biochemical industry, and in particular relates to a method for producing phytosterol from deodorized distillate of vegetable fat by microbial fermentation. The method is characterized in that the deodorized distillate of vegetable fat obtained by microbial fermentation is used, the free fatty acid in the deodorized distillate is used as a nutrient substance of microorganism to be directly metabolized and utilized; glyceride in the deodorized distillate is hydrolyzed into micromolecule substances in the presence of hydrolases such as lipase, esterase and the like, and then the micromolecule substances are absorbed and utilized by microorganism cells so as to be transformed to cell substances; phytosterol combined with free fatty acid and glyceride is released to fermentation broth after free fatty acid and glyceride are digested and utilized, the fermentation broth is subjected to microfiltration so that microorganism cells in the fermentation broth are removed; and because phytosterol is not dissolved in water, filtrate is subjected to low temperature crystallization after concentration so as o separate out phytosterol. The method has the advantages of greatly simplifying the production process, reducing energy consumption and reducing production cost; and at the same time, because an organic solvent is not used, the method dose not generate environmental pollution and has obvious economic benefits.

The invention discloses a health care product for treating uarthritis and a preparation method thereof. The health care product for treating uarthritis is prepared from the following raw materials: wheatgrass extracts, inulin, selenium-enriched yeast, medlar extracts, papain, saussurea involucrate, mentha leaves, glossy ganoderma, radix puerariae extracts, pericarpium citri reticulatae, haws, dicliptera chinensis amd folium mori extracts. The preparation method comprises the steps that the saussurea involucrate, the mentha leaves, the glossy ganoderma, the pericarpium citri reticulatae, the haws and the dicliptera chinensis are soaked in water, and after the soaking is completed, all raw materials are taken out and dried; the dried raw materials are smashed into powder and are uniformly mixed to obtain mixed powder; the mixed powder is poured into wine for sealed soaking, and after the soaking is completed, medicine dregs are filtered to obtain medical wine; the medical wine is concentrated to obtain medicine powder, and the rest raw materials and the medicine powder are uniformly mixed. The health care product has the beneficial effects that the health care product can enhance the metabolism of cell substances in the human body; the osteoporosis and the skeleton aging are prevented; the treatment effect is achieved on the uarthritis; the recurrence of the uarthritis can also be prevented.

The present disclosure is directed to a method of preparing an osteogenic bone graft. The method includes introducing an anticoagulant compound into a volume of cellular material including a mononuclear cell population from the intramedullary canal of a long bone; collecting an effluent including the volume of cellular material containing the anticoagulant compound at a collection point; separating a concentrated cell fraction from the effluent, the concentrated cell fraction including the mononuclear cell population and the anticoagulant compound; and preparing an osteogenic bone graft from the concentrated cell fraction.

The invention discloses a preparation method for a farmhouse poultry and livestock breeding deodorant, and belongs to the technical field of environmental purification materials. The method disclosedby the invention adopts soil next to a poultry and livestock breeding farm as a raw material and screens high-efficiency inorganic chemotrophy-type desulfurization bacteria, the desulfurization bacteria are aerobic bacteria and can utilize residual feed, animal feces and the like in a closed animal house, under the action of microorganisms, sulfur-containing organic substances such as proteins aredecomposed into one or more reduced or partially-reduced sulfur compounds selected from hydrogen sulfide and mercaptan to be used as energy sources, the reduced or partially-reduced sulfur compoundsare oxidized into S, SO3<2-> and SO4<2->, carbon dioxide is used as a main carbon source to synthesize a cell substance, repugnant substances ingested by microbial cells become an energy source of themicroorganisms, the repugnant substances are decomposed, utilized and turned into cell substances by metabolism of the microorganisms to be removed, and sulfur is decomposed; and the method disclosedby the invention solves the problem that a current deodorizing microbial agent has certain damage to human bodies when being used to improve malodorous gases and toxic gases emitted in an animal husbandry breeding place, and cannot effectively suppress viruses, germs and parasites at the same time.

Plant treatment products include a plant treatment component and, preferably mixed with, a microbial fermentation product. The microbial fermentation product includes cellular material of cultured microorganisms and one or more anaerobic metabolite products of the cultured microorganisms. Preferably, the microbial fermentation product comprises a whole culture lysate of a microbial fermentation suspension culture, including liquid fermentation culture medium components and lysed microorganisms. The plant treatment component of the product includes one or more pesticides or plant growth regulators. Plant treatment products can be applied to or around plants or seeds to enhance growth, health, or productivity of the plants.

A composite carbon source production process including nitrogen and phosphorus removing is disclosed and includes phosphorus removing or nitrogen removing. The process includes main steps of a) determining preconditions; b) adding a carbon source; c) determining an addition position; d) determining an addition method; e) determining dosage; f) determining a suitable object, g) determining a concentration judgment standard and h) determining a nitrogen removing manner. The composite carbon source is a composite high-efficiency carbon source which contains nutrients suitable for growth in sewage, and which is developed for industrial or municipal wastewater with high ammonia nitrogen contents. The composite carbon source is rich in nutrition, enables high metabolism vitality of bacterial, ahigh synthesis speed of new cell substances, improved growth rates of denitrifying bacteria, a shortened stagnation period of bacteria and rapid adaption to the environment, and has high tolerance toimpact of outer bad conditions such as salt, temperature and pH values. Through addition of the composite carbon source, competition for carbon sources by denitrifying bacteria and phosphorus accumulating bacteria can be improved, the nitrogen and phosphorus removal efficiency of a low-carbon source sewage treatment biochemical system can be effectively improved, and in particular, the total nitrogen removal effect of sewage can be improved.

A method comprising the steps of: 1. optionally subjecting spermatozoa to a treatment step; 2. subjecting the spermatozoa of step 1 to a sex selection step so as to select for either female or male spermatozoa of interest; 3. carrying out a fertilisation step using the spermatazoa of interest of step 2 to produce at least one oocyte, blastocyst, ovum, embryonic cell or embryo; 4. selectively lysing the at least one oocyte, blastocyst, ovum, embryonic cell or embryo of step 3 in the presence of spermatozoa so as to selectively release cellular material from the at least one lysed oocyte, blastocyst, ovum, embryonic cell or embryo; and 5. using the released cellular material in at least one downstream application.

The present invention provides a method for detecting variant cell-free DNA (cfDNA) in a sample obtained from a subject, where analysis of the sample includes a size-selection step which separates out different fragment sizes of DNA. The sample may be a limited volume sample such as a blood, serum or plasma sample of less than 500ul (e.g. a blood or plasma sample of about 50ul), or other sample that has a low content of cfDNA. The sample may have been stored and/or dried and not have been processed to remove cells or cellular material prior to storage. The size-selection step may comprise filtering-out, depleting or removing genomic DNA (gDNA) fragments of > 200 bp, > 300 bp, > 500 bp, > 700 bp, > 1000 bp, > 1200 bp, > 1500 bp, or > 2000 bp prior to analysis, e.g. prior to DNA sequencing. The method may further comprise performing an analysis that summarises or combines data across multiple loci.

The present disclosure is related to a perfusable-type bio-dual proximal tubule cell construct and a producing method thereof capable of applying an in vitro artificial organ model configured to include a first bioink comprising a decellularized substance derived from a mammalian kidney tissue and human umbilical vascular endothelial cells (HUVECs) and a second bioink comprising the decellularized substance and renal proximal tubular epithelial cells (RPTECs), wherein the first bioink and the second bioink are coaxial and printed in tubular constructs having different inner diameters.

According to the present disclosure, it is possible to use the renal proximal tubule-on-a-chip as a bioreactor capable of observing a biological drug reaction similar to a real drug by perfusing various drugs to the renal proximal tubule-on-a-chip.