43 results about "Cell substance" patented technology

The present invention provides for a novel system of extracting and purifying nucleic acids (DNA, RNA, etc.) from cellular material like blood. Such a system of extraction and purification relies on novel monolithic microfluidic devices and methods of using these devices. Such devices comprise numerous components, monolithically-incorporated on an single chip, and further comprising novel nucleic acid binding materials. The present invention is also directed to method of preparing such novel nucleic binding materials.

In vitro culture devices and methods are described. The subject methods and devices provide a means whereby cells and / or subcellular material are grown or held in a culture device that maintains the cells and / or subcellular material in a physiologically representative environment, thereby improving the predictive value of toxicity and metabolism assays, and the relevance of experimental results derived from such assays to actual in vivo conditions, processes and outcomes. The culture devices of the invention comprise a fluidic channel connected to or otherwise integrated with at least one chamber, preferably integrated in a chip format. The specific chamber geometry is designed to provide cellular interactions, liquid flow, and liquid residence and other parameter values that correlate with those found in or produced by the corresponding cell, organs or tissues, or components or products thereof, in vivo. Each device comprises at least one chamber and at least one inlet and one outlet port that allow for recirculation of the culture medium. The device will usually include a mechanism for obtaining signals from the cells and culture medium.

The invention provides a spray nozzle with double flow channels. The spray nozzle comprises a spray nozzle unit with the inner flow channel, a spray nozzle unit with the outer flow channel, a connector and a spray nozzle body. The spray nozzle unit with the inner flow channel and the spray nozzle unit with the outer flow channel are connected to the spray nozzle body by the connector, and a spinneret structure is formed in the spray nozzle body in such a manner that an inner-layer material squeezed out of the spray nozzle unit with the inner flow channel is positioned in the center of the spinneret structure and an outer-layer material squeezed out of the spray nozzle unit with the outer flow channel wraps the periphery of the spinneret structure. The spray nozzle with the double flow channels has the advantages that the two different materials are respectively arranged in the inner flow channel and the outer flow channel by the aid of inner and outer double-flow-channel technologies, accordingly, the spinneret structure with the corresponding material in the center and the other material uniformly arranged on the periphery can be formed by means of squeezing, the central material can be effectively protected, and cell substances in the center of the spray nozzle can be protected when the spray nozzle is applied to 3D (three-dimensional) biological printing; nutrients can be provided, so that the survival rate of printed biological cells can be increased.

Provided are nanoparticles, method of using and making thereof. The invention nanoparticle comprises a) an inner core comprising a non-cellular material; and b) an outer surface comprising a cellular membrane derived from a cell or a membrane derived from a virus. Medicament delivery systems or pharmaceutical compositions comprising the inventive nanoparticles are also provided. The present invention further provides immunogenic compositions comprising the inventive nanoparticles, and methods of use the inventive immunogenic compositions for eliciting an immune response and for treating or preventing disease or condition, such as neoplasm or cancer, or disease or conditions associated with cell membrane inserting toxin. Vaccines comprising the immunogenic composition comprising the nanoparticles of the present invention are also provided.

The invention relates to a confocal-photoacoustic dual-mode microscopic imaging method which comprises the following steps of: (1) carrying out confocal microscopic imaging to an observed object and carrying out photoacoustic scanning imaging to the observed object; and (2) acquiring the functional image of biological tissue by comparing dual-mode images. The invention also relates to a confocal-photoacoustic dual-mode microscopic imaging device which comprises a confocal microscopic imaging system and a photoacoustic scanning imaging system, wherein the confocal microscopic imaging system mainly comprises a laser, a laser scanning galvanometer, a pinhole and a photomultiplier, and the photoacoustic scanning imaging system is formed by sequentially and electrically connecting a photoacoustic sensor, a chopper, a phase-locked amplifier, a data acquisition card and a computer. The invention can carry out confocal-photoacoustic dual-mode microscopic imaging to cells for acquiring the structure information, the content space distribution information and the function information of the cells and the dynamic distribution information of the cell substance contents.

The invention relates to a therapeutic composition, a method for producing a therapeutic composition, and the use of a cell-free substance, especially a cell-free bone or cartilage matrix. The disclosed therapeutic composition comprises at least a cell-free substance obtained from stimulated stem cells and / or precursor cells. Immunogenic reactions during in vivo therapeutic use are prevented by the fact that the therapeutic composition is free from cells and contains no typically antigenic cell components. The disclosed composition can therefore be universally used for the therapeutic purposes regardless of the origin of the stem cells and / or precursor cells and utilize the natural regenerative potency thereof in a highly efficient manner for replacing tissue, e.g. for a suitable bone and / or cartilage structure.

This invention provides an analytic method of inner micro-fluidic chip cell substance, which is characterized by the following: introducing biotin and affinity system; fixing the cell at a specific position of microchannel, which is decomposed by chemical reagent; making biotin- affinity protein micro-adsorption mode on the surface of microchannel; utilizing the special interaction of biotin and affinity; fitting biotin cell on the specific position of microchannel surface; decomposing the specific cell in the chemical buffer solution with high voltage; detecting the inner discharging substance by electrophoresis separation. This method realizes single cell sampling, operating, decomposing, separating and detecting by simple device, which is convenient and feasible for micro-fluidic chip cell analysis and decomposition.

The invention discloses a method for co-culturing, inducing and amplifying gamma delta T cells and NK cells. The gamma delta T cells and the NK cells are obtained in a co-culture manner; peripheral blood is collected to separate and screen mononuclear cells and stimulate and induce the mononuclear cells to induce the gamma delta T cells and the NK cells, the gamma delta T cells and the NK cells are subjected to activation amplification culture to amplify a gamma delta T cell and NK cell composite cell substance having characteristics of large number, high amplification multiple, strong cytotoxicity and the like so as to form the co-culture cell composition through compounding, and the co-culture cell composition has good clinical application value. The co-culture induced amplification method improves the cell culture efficiency, greatly reduces the culture cost, reduces the difficulty of a culture technology, simplifies the clinical treatment process, reduces the treatment cost, and simplifies the treatment means.

The invention belongs to the field of biochemical industry, and in particular relates to a method for producing phytosterol from deodorized distillate of vegetable fat by microbial fermentation. The method is characterized in that the deodorized distillate of vegetable fat obtained by microbial fermentation is used, the free fatty acid in the deodorized distillate is used as a nutrient substance of microorganism to be directly metabolized and utilized; glyceride in the deodorized distillate is hydrolyzed into micromolecule substances in the presence of hydrolases such as lipase, esterase and the like, and then the micromolecule substances are absorbed and utilized by microorganism cells so as to be transformed to cell substances; phytosterol combined with free fatty acid and glyceride is released to fermentation broth after free fatty acid and glyceride are digested and utilized, the fermentation broth is subjected to microfiltration so that microorganism cells in the fermentation broth are removed; and because phytosterol is not dissolved in water, filtrate is subjected to low temperature crystallization after concentration so as o separate out phytosterol. The method has the advantages of greatly simplifying the production process, reducing energy consumption and reducing production cost; and at the same time, because an organic solvent is not used, the method dose not generate environmental pollution and has obvious economic benefits.

A process for obtaining a substantially pure, stable solution of a molluscan haemocyanin, comprising the steps of: (1) collecting blood from a mollusc; (2) centrifuging the collected blood to remove cellular material and other particulates to obtain a serum fraction; (3) diafiltering the serum fraction to replace serum liquid with a cation exchange equilibration buffer using an ultrafiltration membrane; (4) contacting the serum fraction with a strong cation exchange resin in the presence of an equilibration buffer with a pH between 4.0 and 6.5, whereby a protein component of the serum fraction is retained on the column; (5) eluting the retained protein component from the column with a high salt elution buffer; and (6) concentrating the eluate and exchanging the elution buffer for a pH neutral / low salt diafiltration buffer by recirculating the eluate through an ultrafiltration membrane in the presence of the diafiltration buffer.

The invention discloses a health care product for treating uarthritis and a preparation method thereof. The health care product for treating uarthritis is prepared from the following raw materials: wheatgrass extracts, inulin, selenium-enriched yeast, medlar extracts, papain, saussurea involucrate, mentha leaves, glossy ganoderma, radix puerariae extracts, pericarpium citri reticulatae, haws, dicliptera chinensis amd folium mori extracts. The preparation method comprises the steps that the saussurea involucrate, the mentha leaves, the glossy ganoderma, the pericarpium citri reticulatae, the haws and the dicliptera chinensis are soaked in water, and after the soaking is completed, all raw materials are taken out and dried; the dried raw materials are smashed into powder and are uniformly mixed to obtain mixed powder; the mixed powder is poured into wine for sealed soaking, and after the soaking is completed, medicine dregs are filtered to obtain medical wine; the medical wine is concentrated to obtain medicine powder, and the rest raw materials and the medicine powder are uniformly mixed. The health care product has the beneficial effects that the health care product can enhance the metabolism of cell substances in the human body; the osteoporosis and the skeleton aging are prevented; the treatment effect is achieved on the uarthritis; the recurrence of the uarthritis can also be prevented.

The invention discloses a gynecological fluorescent staining solution and a preparation method and application thereof.The gynecological fluorescent staining solution comprises acridine orange, sodium chloride, EDTA-Na, disodium hydrogen phosphate and citric acid, the components can be combined with different cell substances for staining, so that fluorescence of different colors is displayed under the action of exciting light, the components are clear after staining, and the staining effect is good. Different components present different colors and are easy to identify and distinguish. The preparation method of the gynecological fluorescent staining solution is simple, and the gynecological fluorescent staining solution can be prepared and used at any time and can also be stored and used after being prepared. According to the use method of the gynecological fluorescent staining solution, two nucleic acids, namely DNA and RNA, are mainly displayed, so that the fluorescent staining solution is obvious in luminescence. The use method is simple, observation and detection can be carried out through a fluorescence microscope after the gynecological fluorescent staining solution is dropwise added, the defects of a traditional saline smear method are overcome, and the detection rate is increased.

The invention belongs to the field of biochemical industry, and in particular relates to a method for producing phytosterol from deodorized distillate of vegetable fat by microbial fermentation. The method is characterized in that the deodorized distillate of vegetable fat obtained by microbial fermentation is used, the free fatty acid in the deodorized distillate is used as a nutrient substance of microorganism to be directly metabolized and utilized; glyceride in the deodorized distillate is hydrolyzed into micromolecule substances in the presence of hydrolases such as lipase, esterase and the like, and then the micromolecule substances are absorbed and utilized by microorganism cells so as to be transformed to cell substances; phytosterol combined with free fatty acid and glyceride is released to fermentation broth after free fatty acid and glyceride are digested and utilized, the fermentation broth is subjected to microfiltration so that microorganism cells in the fermentation broth are removed; and because phytosterol is not dissolved in water, filtrate is subjected to low temperature crystallization after concentration so as o separate out phytosterol. The method has the advantages of greatly simplifying the production process, reducing energy consumption and reducing production cost; and at the same time, because an organic solvent is not used, the method dose not generate environmental pollution and has obvious economic benefits.

The invention provides a spray nozzle with double flow channels. The spray nozzle comprises a spray nozzle unit with the inner flow channel, a spray nozzle unit with the outer flow channel, a connector and a spray nozzle body. The spray nozzle unit with the inner flow channel and the spray nozzle unit with the outer flow channel are connected to the spray nozzle body by the connector, and a spinneret structure is formed in the spray nozzle body in such a manner that an inner-layer material squeezed out of the spray nozzle unit with the inner flow channel is positioned in the center of the spinneret structure and an outer-layer material squeezed out of the spray nozzle unit with the outer flow channel wraps the periphery of the spinneret structure. The spray nozzle with the double flow channels has the advantages that the two different materials are respectively arranged in the inner flow channel and the outer flow channel by the aid of inner and outer double-flow-channel technologies, accordingly, the spinneret structure with the corresponding material in the center and the other material uniformly arranged on the periphery can be formed by means of squeezing, the central material can be effectively protected, and cell substances in the center of the spray nozzle can be protected when the spray nozzle is applied to 3D (three-dimensional) biological printing; nutrients can be provided, so that the survival rate of printed biological cells can be increased.

The invention discloses a healthcare Salicornia Bigelovii Torr. and sesame seed oil and a making method thereof. The healthcare Salicornia Bigelovii Torr. and sesame seed oil meets people's demands for nutrition, healthcare and deliciousness. A technical scheme of ultrasonic assisted wall breaking, biological isomerization reaction and carbon dioxide supercritical extraction is adopted to make the above product rich in conjugated linoleic acid, alpha-linolenic acid and other functional oils, so the product has good anti-oxidation ability and nutrition and healthcare effects; and the product can be used for a long term, and has the efficacy of nourishing the liver and the kidney, strengthening muscles and bones, reducing cholesterols, promoting fat reduction, removing wastes in bodies, enhancing human body cell substance metabolism, balancing the blood pressure of human bodies and improving the immunity of the human bodies. The making method has the advantages of mild reaction conditions, short production time, low production cost, easiness in large-scale production, realization of high content of conjugated linoleic acid and alpha-linolenic acid, high anti-oxidation ability and good healthcare effects of the product, and wide industrial promotion prospect.

The invention discloses a preparation method of an exosome for nanoflow cytometry detection, and belongs to the technical field of cell substance detection. The preparation method comprises the following steps: (1) taking an exosome sample; (2) carrying out on-ice unfreezing treatment on the exosome sample; (3) performing ultrasonic treatment on the exosome sample; and (4) adding PBS into a centrifugal tube, then adding an antibody into the centrifugal tube, fully and uniformly mixing the mixture, then adding the exosome sample, performing incubating, and carrying out centrifugal treatment by an ultra-high-speed centrifuge to obtain the PBS resuspended exosome. The invention discloses the preparation method of the exosome for nanoflow cytometry detection. The method is simple to operate, accurate, reliable, high in sensitivity, free of extraction by an organic solvent and high in selectivity to a target detection object.

The present disclosure is directed to a method of preparing an osteogenic bone graft. The method includes introducing an anticoagulant compound into a volume of cellular material including a mononuclear cell population from the intramedullary canal of a long bone; collecting an effluent including the volume of cellular material containing the anticoagulant compound at a collection point; separating a concentrated cell fraction from the effluent, the concentrated cell fraction including the mononuclear cell population and the anticoagulant compound; and preparing an osteogenic bone graft from the concentrated cell fraction.

The invention discloses a preparation method for a farmhouse poultry and livestock breeding deodorant, and belongs to the technical field of environmental purification materials. The method disclosedby the invention adopts soil next to a poultry and livestock breeding farm as a raw material and screens high-efficiency inorganic chemotrophy-type desulfurization bacteria, the desulfurization bacteria are aerobic bacteria and can utilize residual feed, animal feces and the like in a closed animal house, under the action of microorganisms, sulfur-containing organic substances such as proteins aredecomposed into one or more reduced or partially-reduced sulfur compounds selected from hydrogen sulfide and mercaptan to be used as energy sources, the reduced or partially-reduced sulfur compoundsare oxidized into S, SO3<2-> and SO4<2->, carbon dioxide is used as a main carbon source to synthesize a cell substance, repugnant substances ingested by microbial cells become an energy source of themicroorganisms, the repugnant substances are decomposed, utilized and turned into cell substances by metabolism of the microorganisms to be removed, and sulfur is decomposed; and the method disclosedby the invention solves the problem that a current deodorizing microbial agent has certain damage to human bodies when being used to improve malodorous gases and toxic gases emitted in an animal husbandry breeding place, and cannot effectively suppress viruses, germs and parasites at the same time.

Plant treatment products include a plant treatment component and, preferably mixed with, a microbial fermentation product. The microbial fermentation product includes cellular material of cultured microorganisms and one or more anaerobic metabolite products of the cultured microorganisms. Preferably, the microbial fermentation product comprises a whole culture lysate of a microbial fermentation suspension culture, including liquid fermentation culture medium components and lysed microorganisms. The plant treatment component of the product includes one or more pesticides or plant growth regulators. Plant treatment products can be applied to or around plants or seeds to enhance growth, health, or productivity of the plants.

Plant treatment products include a plant treatment component and, preferably mixed with, a microbial fermentation product. The microbial fermentation product includes cellular material of cultured microorganisms and one or more anaerobic metabolite products of the cultured microorganisms. Preferably, the microbial fermentation product comprises a whole culture lysate of a microbial fermentation suspension culture, including liquid fermentation culture medium components and lysed microorganisms. The plant treatment component of the product includes one or more pesticides or plant growth regulators. Plant treatment products can be applied to or around plants or seeds to enhance growth, health, or productivity of the plants.

A composite carbon source production process including nitrogen and phosphorus removing is disclosed and includes phosphorus removing or nitrogen removing. The process includes main steps of a) determining preconditions; b) adding a carbon source; c) determining an addition position; d) determining an addition method; e) determining dosage; f) determining a suitable object, g) determining a concentration judgment standard and h) determining a nitrogen removing manner. The composite carbon source is a composite high-efficiency carbon source which contains nutrients suitable for growth in sewage, and which is developed for industrial or municipal wastewater with high ammonia nitrogen contents. The composite carbon source is rich in nutrition, enables high metabolism vitality of bacterial, ahigh synthesis speed of new cell substances, improved growth rates of denitrifying bacteria, a shortened stagnation period of bacteria and rapid adaption to the environment, and has high tolerance toimpact of outer bad conditions such as salt, temperature and pH values. Through addition of the composite carbon source, competition for carbon sources by denitrifying bacteria and phosphorus accumulating bacteria can be improved, the nitrogen and phosphorus removal efficiency of a low-carbon source sewage treatment biochemical system can be effectively improved, and in particular, the total nitrogen removal effect of sewage can be improved.

The present invention provides nanoparticles and methods of their use and manufacture. Nanoparticles of the invention comprise a) an inner core comprising non-cellular material; and b) an outer surface comprising a cell-derived cell membrane or a virus-derived membrane. Also provided are drug delivery systems or pharmaceutical compositions comprising the nanoparticles of the invention. The present invention further provides immunogenic compositions comprising nanoparticles of the present invention and the use of immunogenic compositions of the present invention for eliciting an immune response and for treating or preventing diseases or conditions such as tumors or cancer, or in combination with inserted A method for a disease or condition associated with a toxin of the cell membrane. The invention also provides a vaccine comprising said immunogenic composition comprising a nanoparticle of the invention.

Provided herein are scaffold biomaterials comprising a decellularized plant or fungal tissue from which cellular material and nucleic acid of the tissue are removed, the decellularized plant or fungal tissue having a 3-dimensional porous structure; wherein the decellularized plant or fungal tissue may optionally be at least partially coated or mineralized, and wherein the scaffold biomaterial may optionally further comprise a protein-based hydrogel and / or a polysaccharide-based hydrogel, or both. Also provided herein are methods and uses of such scaffold biomaterials, e.g., including methods of manufacture and methods and uses for bone tissue engineering.

A method comprising the steps of: 1. optionally subjecting spermatozoa to a treatment step; 2. subjecting the spermatozoa of step 1 to a sex selection step so as to select for either female or male spermatozoa of interest; 3. carrying out a fertilisation step using the spermatazoa of interest of step 2 to produce at least one oocyte, blastocyst, ovum, embryonic cell or embryo; 4. selectively lysing the at least one oocyte, blastocyst, ovum, embryonic cell or embryo of step 3 in the presence of spermatozoa so as to selectively release cellular material from the at least one lysed oocyte, blastocyst, ovum, embryonic cell or embryo; and 5. using the released cellular material in at least one downstream application.

The method for the diagnosis of a microbial infection in an organism makes use thereof that said microbial infection is at least partially present as elementary bodies in cellular material of the organism. The method comprises the steps of (1) providing a sample of cellular material from the organism; (2) processing the sample to obtain a test composition, that is enriched in elementary bodies, inso far as the sample contains any elementary bodies; and (3) Subjecting a volume of the test composition comprising at most a predetermined maximum of elementary bodies to a MALDI mass spectrometry method to identify presence of the microbial infection. The sample processing more particularly involves cell lysis and separation of elementary bodies from the lysed cell material. Subsequently, the elementary bodies (and any further material attached thereto) are contacted with a matrix material, that facilitates the MALDI mass spectrometry.

The present application relates to a cell imaging composition comprising 1-(o-benzoylphenylaminoalkyl)-phenylboronic acid [1-(ormo-benzoylphenoalkyl)-phenylboronic acid] or a derivative thereof as a fluorescent probe compound, and a method for imaging a cell substance using the same. The present invention also relates to a method for imaging a cell substance using the composition.

Systems and methods for developing and applying photosynthetic cellular substances to a human or animal for medical, therapeutic or cosmetic uses are provided. Photosynthetic cells, such as algal cells, can be used in these substances to provide the ability to continuously generate oxygen when exposed to a light source or other oxygen-generating trigger. The substances can be developed as a standalone liquid, gel or cream, or embedded within a bandage, mesh, scaffold or other structure with a light source to promote oxygen production. The substances can be applied topically for medical, therapeutic or cosmetic treatments, or injected internally for generation of oxygen within one or more parts of the body.

The invention relates to the technical field of sewage treatment, in particular to a novel composite carbon source additive and a preparation method thereof. The additive comprises the following components in percentage by weight: 8%-20% of 1, 2-ethylidene glycol (ethylene glycol), 8%-30% of methyl glycol, 8%-30% of sodium acetate, 8%-25% of propylene glycol methyl ether acetate, 8%-25% of protein high peptide and amino acid and the balance of water. The novel composite carbon source additive disclosed by the invention can provide nutrients required by microorganisms, realize high-efficiency denitrification of wastewater and enhance the metabolic activity of bacteria, so that the speed of synthesizing a new cell substance and the utilization rate of a carbon source matrix are increased, the biological denitrification efficiency is further improved, and the requirements of the market on a high-quality and high-efficiency water treatment carbon source are met.

The present invention provides a method for detecting variant cell-free DNA (cfDNA) in a sample obtained from a subject, where analysis of the sample includes a size-selection step which separates out different fragment sizes of DNA. The sample may be a limited volume sample such as a blood, serum or plasma sample of less than 500ul (e.g. a blood or plasma sample of about 50ul), or other sample that has a low content of cfDNA. The sample may have been stored and / or dried and not have been processed to remove cells or cellular material prior to storage. The size-selection step may comprise filtering-out, depleting or removing genomic DNA (gDNA) fragments of > 200 bp, > 300 bp, > 500 bp, > 700 bp, > 1000 bp, > 1200 bp, > 1500 bp, or > 2000 bp prior to analysis, e.g. prior to DNA sequencing. The method may further comprise performing an analysis that summarises or combines data across multiple loci.