The invention discloses a multielement nanometer spray for operating room instrument sterilization and a preparing method thereof. The spray is prepared from propylene glycol, phosphorous acid, nanometer medical stone meal, sodium citrate benzotriazole, fluvastatin, zinc sulfate, iris florentina extract, polygonum orientale extract, aloe-emodin, jasmonic acid, laggera alata extract, 2-phenoxetol, prothioconazole, 4-chloro-3,5-dimethylphenol, iprodione, phosphorous acid, trifloxystrobin, alkyne tert-butyl carbamate, emulgator, defoamer, surfactant, liquid stabilizer, ethanol and deionized water. The spray can achieve antibiosis, lubrication and corrosion resistance, and can be widely applied to operating room instrument sterilization to effectively kill various bacteria and viruses with generating residues and prevent hospital cross infection.