The invention belongs to the field of identification of
pichia aldriavzevii, and particularly relates to an identification method and application of
pichia aldriavzevii with high tolerance, reduced
malic acid and
ethanol production, and the preservation number of the
pichia aldriavzevii strain is CGMCC No. 22929. The identification method comprises the following steps: carrying out
molecular identification through ITS sequence
amplification sequencing; the temperature,
ethanol, acid and
osmotic pressure tolerance is evaluated by measuring the growth condition of the strain in a YPD culture medium containing
ethanol, pH-
malic acid combination and
sodium chloride at different temperatures; by measuring the
malic acid degradation rate and the
ethanol yield of the strain in a malic acid-containing culture medium, the acid reduction and ethanol production capacities of the strain are evaluated. The strain can tolerate the high temperature of 50 DEG C, 15.2% vol ethanol, pH 3.5 and 46.8 g / L
sodium chloride, and can effectively degrade malic acid and improve the yield of ethanol. The method is suitable for solving the problem of food
fermentation caused by too high malic
acid content and has good application prospects.