Process for solid phase cultivating preserving of Laminaria gamobium

Abstract

The invention discloses a kelp gametophyte solid phase culturing storing method. It adopts sea belt male and female gametophyte clone, and includes the following steps: preparing solid phase culturing culture medium; smashing gametophyte clone; inoculating gametophyte; solid phase culturing; solid phase storing and resuscitation enlarging foster. The invention can make liquid culture storing long, anaphase operation simple, and provides the possibility of applying spaceflight breeding technique in marine alga breeding.

Description

1. Technical field [0001] The invention belongs to the technical field of marine biology, and in particular relates to a method for solid-phase preservation of kelp gametocytes of macroeconomic algae-kelp germplasm. 2. Background technology [0002] Kelp (Laminaria japonica Aresch) is an important economic algae in China. The life history of kelp is divided into two distinct generational stages: the sporophyte generation and the gametophyte generation. Separation culture can form asexual reproduction lines. In the 1970s, Fang Zongxi and others successfully separated and cultivated the male and female gametophytes of Laminaria, and established asexual reproduction lines, also known as cloning. Cui Jingjin et al. conducted research on the preservation of kelp gametophytes under low light. Wu Chaoyuan et al. proposed that the factors suitable for the growth of gametophyte clones are: temperature 10-15°C, light 2000-30001x, N6-8g / m 3 , P0.8-1g / m 3 . The traditional germplasm...

AI Technical Summary

Problems solved by technology

The traditional germplasm preservation technology is to subculture and preserve the clones. The traditional preservation technology has obvious deficiencies: (1) liquid culture is the only preservation method, and the method is single, which limits the scale and efficiency of preservation; (2) liquid culture Preservation generally needs to update the culture medium within half a month, the workload is heavy, the operation is cumbersome, and frequent operations also increase the chance of germplasm contamination and mixing; (3) the kelp species cannot be aseptically treated before the spores are collected, and the entire preservation process is difficult. Under the condition of bacteria, the pollution of microorganisms, especially algae, cannot be completely eliminated; (4) Abnormal cell morphology and parthenogenesis occur from time to time during culture, and the culture factors need to be further optimized

Therefore, the current seed conservation technology is a traditional method of subculture preservation, which cannot completely rule out the possibility of mixing, contamination, and variation.

[0003] These shortcomings limit the application of Laminaria gametophyte clones as the main materials for Laminaria seedlings and breeding

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