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Inoculating method of tomato pathogenic bacterium

A technology of pathogenic bacteria and pathogenic bacteria, which is applied in the field of inoculation of plant pathogenic bacteria, can solve the problems of unsuitable identification of canker resistance, instability of leaf-cutting method, small inoculation amount of acupuncture method, etc., to achieve stable and uniform inoculation amount, Difficult to operate and small amount of inoculated bacteria

Inactive Publication Date: 2008-02-13
CHINA AGRI UNIV
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  • Summary
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Several traditional inoculation methods have different defects when they are used to inoculate Cmm at the tomato seedling stage, such as leaf-cutting method is unstable; The (dip) root method is complicated to operate and is not suitable for experiments with large sample volumes, etc.
After the inventor tried several reported common bacterial disease inoculation methods, he thought that these inoculation methods were not suitable for inoculating Cmm at the tomato seedling stage and carrying out the anti-canker identification of tomato at the seedling stage

Method used

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Examples

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Effect test

Embodiment 1

[0014] Taking tomato bacterial canker bacterium as an example, the inoculation method of the present invention comprises the steps:

[0015] (1) Sowing and seedling management: sow healthy tomato seeds in seedling trays filled with sterilized substrates, water properly to maintain the humidity of the substrates, and manage them normally under greenhouse conditions. 25 days after sowing, depending on the climate Sunshine and other conditions are all feasible in the range of 21-28 days, that is, when the tomato seedlings grow to the stage of 3-4 true leaves, they are used as inoculated recipient plants.

[0016] (2) Preparation of inoculum: inoculate tomato canker sore bacteria on YDC medium, that is, yeast extract, glucose, and calcium carbonate medium, and culture it at 28°C for 60 hours, and the culture time is 48 to 72 hours. Feasible, then with sterile saline containing 0.85% NaCl to prepare a concentration of 10 8 The bacterial suspension, the bacterial suspension concent...

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Abstract

This invention relates to an inoculation method for tomato pathogenetic bacteria. The method comprises the steps of: preparing 108-109 cfu / mL of tomato pathogenetic bacteria suspension for inoculation, and cutting the stems of tomato seedlings by using sterilized surgical scissors dipped with the suspension so that the seedlings are invaded by the pathogenetic bacteria. This invention can rapidly and easily inoculate pathogenetic bacteria to tomato seedlings, and is suitable for large scale inoculation.

Description

technical field [0001] The invention relates to an inoculation method of plant pathogenic bacteria, in particular to an inoculation method of tomato pathogenic bacteria. Background technique [0002] Common inoculation methods for plant pathogenic bacteria include leaf-cutting method, dipping (dipping) root method, acupuncture method, etc. Each method has its own advantages and disadvantages, and different inoculation methods should be used to study different bacterial diseases. The more important tomato bacterial diseases in production include tomato canker, bacterial wilt, bacterial spot and scab. Among them, tomato bacterial canker is a very important and destructive disease in tomato production in the world. The pathogen is Clavibactermichiganensis subsp.michiganensis (hereinafter referred to as Cmm), which is a quarantine pest in China; the main mode of transmission of the pathogen is seed transmission At present, there is no effective control method for tomato bacteri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 李健强罗来鑫刘西莉吴学宏刘鹏飞张文华
Owner CHINA AGRI UNIV