Soybean gray leaf spot detached leaf identification method
An isolated leaf and identification method technology, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, and microorganism determination/inspection, can solve the problems of poor repeatability of field identification and low accuracy of identification results, and save labor. and financial resources, high accuracy, and the effect of disease-prone grades
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preparation example 1
[0041] Preparation example 1 prepares gray spot pathogen spore suspension
[0042] The gray spot pathogenic bacteria stored in a refrigerator at 4°C (Northeast Agricultural University Plant Pathology Teaching and Research Office) was inoculated on a flat PDA medium, and activated for 7 days (cultivation conditions: cultured in a constant temperature incubator at 25°C for 7 days). The activated cinerea was cut into small pieces along with the PDA medium agar block, and inoculated on the sorghum grain medium, and multiplied and cultured for 14 days (the culture conditions were in a constant temperature incubator, 25°C for 14 days).
[0043] Take 5 bottles of sorghum grain culture medium that has been multiplied at a time (the volume of diseased grains in the bottle is 100ml), pour the sorghum grains in the bottle into a 2000ml plastic beaker, and the volume of sorghum grains in the beaker can be observed to be about 500ml. Add 225ml of distilled water, just enough to make the ba...
preparation example 2
[0045] Preparation example 2 prepares the environment that keeps blade normal survival
[0046] Making the pelvic floor supports of standard identification white basins
[0047] Clean the toughened plastic white basin with an inner diameter of 41.5×27.5×5.5cm and an outer diameter of 46×32×5.5cm, take a gauze roll with a width of about 20.5cm (the unfolded width is 82cm) and a roll of absorbent cotton with a width of about 30cm, Identify the length of the inner diameter of the white basin according to the standard, and cut off a piece of gauze about 41.5 cm long; according to the length and width of the inner diameter of the white basin, tear off the required absorbent cotton. Spread the cut gauze horizontally in the identification white basin, spread the torn absorbent cotton evenly over the entire surface of the gauze, the thickness of the absorbent cotton is about 9mm, then put the unfolded gauze back into the basin and place it on the absorbent cotton, so that The absorbe...
Embodiment 1
[0050] Plant material and strains tested
[0051] The plant material is the susceptible variety soybean Hefeng 45, and the tested strain is the No. 7 physiological race of dominant gray spot disease in Heilongjiang Province (Department of Phytopathology, Northeast Agricultural University).
[0052] Select fresh soybeans with similar growth potential at the same joint to fully unfold the leaves, cut off the three compound leaves with stalks, and then cut off the three leaflets of the three compound leaves in order, rinse them with sterile water, and divide them according to the variety number. Neatly and orderly place the leaves face up on the surface of the surface gauze in the standard identification white basin, leaving a blank distance of 5-7cm in the top, bottom, left, and right sides. When placing, care should be taken to gently press the petiole of the leaf downward so that the petiole of the leaf is in close contact with the support at the bottom of the basin, so that t...
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