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Method for breeding strand cheeseflower utilizing cell engineering rapid-breeding technology

A technology of mallow and tissue culture, applied in the directions of plant cells, horticultural methods, botanical equipment and methods, etc., can solve the problems of multiplying high-yield and high-quality seaside mallow seedlings, shortening the breeding cycle, etc., so as to shorten the breeding cycle and increase agricultural production. land use effect

Inactive Publication Date: 2008-04-23
NANJING UNIV
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Problems solved by technology

[0004] At present, there are no studies and related reports on the use of cell engineering rapid propagation technology to rapidly propagate high-yield and high-quality seaside mallow seedlings and provide research materials for genetic breeding and shorten the breeding cycle.

Method used

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  • Method for breeding strand cheeseflower utilizing cell engineering rapid-breeding technology

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specific Embodiment approach

[0027] The yield and quality of seaside mallow seeds can be improved by genetic breeding, but high-yield and high-quality plants or seeds are rarely obtained. The explants of seaside mallow plants are used to induce regeneration plants, and a large number of high-yield and high-quality seaside mallow plants are propagated. The specific implementation is as follows:

[0028] (1) Plant regeneration through seeds: select seeds produced by excellent plants cultivated by genetic breeding. The seeds are first sterilized. The specific steps are: 75% alcohol for 1min, distilled water rinse 5 times, 0.1% HgCl 2 10min, rinsed with distilled water 5 times, and then inserted into the culture medium. When the seed germinates to about 8 cm, the stem section with axillary buds is cut and inserted into the plant regeneration medium for cultivation. After 20 days, a large number of regenerated plants can be cultivated, and these regenerated plants can continue to be used for mass reproduct...

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Abstract

The present invention belongs to the field of plant tissue culture technology. Said invention relates to a method for propagating strand malva sinensis by utilizing cell engineering quick propagation technique. Said malva sinensis can be grown in the adverse environment, and the oil content of its seed and fat content are high and similar to that of soybean. Said invention provides the concrete method and steps for quickly propagating strand malva sinensis.

Description

1. Technical field [0001] The invention belongs to the technical field of plant tissue culture 2. Background technology [0002] my country has more than 500 million mu of saline soil resources, and there are about 300 million mu of coastal tidal flats to be developed. This requires the research and introduction of a variety of salt-tolerant plants with important economic value, among which seaside mallow is a kind of salt-tolerant plant. Oil plants have broad prospects for development. [0003] Seashore mallow (Kosteletzkya virginica (L.) presl.) is native to the United States and is a perennial plant that is naturally distributed in the salt marsh coastal zone from Delaware to Texas in the eastern United States. low soil moisture content), barren, windy, blowing sand, unstable substrates and high salinity environments. The oil content and crude fat content of its seeds are similar to those of soybean, an important economic crop. The protein content is slightly lower than ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/04A01G7/00
Inventor 钦佩周军王光韩睿明
Owner NANJING UNIV