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Curly pondweed rigid bud tissue culturing method

A technology of tissue culture and weed grass, which is applied in the field of plant tissue culture, can solve the problems of stone bud growth being restricted by seasons, difficult to germinate, difficult to propagate on a large scale, etc.

Inactive Publication Date: 2007-03-14
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the seeds are extremely difficult to germinate under natural conditions, the growth of stone buds is limited by seasons and the germination rate is low, it is difficult to propagate on a large scale.
[0004] At present, there is no relevant report on the research on the tissue culture technology of Smilax at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] 1. Establish a sterile system

[0020] After washing the Potamogeton buds with tap water, soak them in 70% ethanol for 30s and use 0.1% HgCl 2 The aqueous solution contains a small amount of Tween 80, sterilized for 9 minutes, and then washed with sterile distilled water 5 times. The sterilized stone buds were inoculated on MS medium without exogenous hormones supplemented with 3% sucrose, and a sterile system was established under the conditions of a temperature of 25±2° C., a light intensity of 2000 lx, and a daily light for 12 hours.

[0021] 2. Tissue culture method of Potamogeton crispus stone bud

[0022] Adventitious bud induction culture is carried out by using Potamogeton crispus buds. Through hormone regulation, the frequency of adventitious bud induction can be increased to 100%.

[0023] The establishment of a sterile system is the key to determining whether it can smoothly induce differentiation. By adjusting and improving the sterilization conditions of explan...

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Abstract

The invention relates to the technology field of plant tissue culture, wherein potamogeton crispus turions tissue culture and rapid multiplication technology technique is employed for the mass production of high grade potamogeton crispus tissue culture sprouts, which has overcome the problem of different seed germination under natural conditions, thus meeting the need of recovering aquatic vegetation, developing herbivorous fishes, purifying water and introducing seeds into lakes and ponds.

Description

1. Technical Field [0001] The invention belongs to the technical field of plant tissue culture. 2. Background technology [0002] my country's water ecosystems are seriously polluted, and the decline in water environment quality has caused serious damage to aquatic biological resources. It is of great significance to restore the balance of aquatic ecosystems, purify water quality, promote the development of fisheries, and introduce water purification plants. [0003] Potamogeton crispus L. (Potamogeton crispus L.) is a perennial submerged herbaceous plant of Potamogetonaceae, which has great nutritional and economic value. Potamogeton crispus has strong adaptability to water eutrophication, and can grow densely in water bodies that are heavily polluted by domestic sewage. It has the function of purifying water bodies and is the main aquatic plant to purify water quality from winter to early summer. However, because the seeds are extremely difficult to germinate under natural cond...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 安树青郑丽屏蒋金辉姚鑫黄成
Owner NANJING UNIV