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Preparation method of umbellate pore fungus polysaccharide sulphate

Polyporus polysaccharide and sulfate technology are applied in medical preparations, pharmaceutical formulas, digestive systems and other directions containing active ingredients to achieve the effects of improving humoral immunity and cellular immunity, reducing pathological damage, and promoting the production of

Inactive Publication Date: 2008-07-23
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no literature report about the synthesis of Polyporus polysaccharide sulfate and its effect on the biological activity of Polyporus polysaccharide

Method used

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  • Preparation method of umbellate pore fungus polysaccharide sulphate
  • Preparation method of umbellate pore fungus polysaccharide sulphate
  • Preparation method of umbellate pore fungus polysaccharide sulphate

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0020] 1. Instrument:

[0021] Stainless steel pot, electric furnace, glass rod, beaker (1000ml), measuring cylinder (1000ml), analytical balance, Newley balance, Erlenmeyer flask, thermometer, dialysis bag, pipette (10ml, 5ml), three-necked flask, spherical condenser , separatory funnel, dropping funnel, agitator, TDL-5 desktop centrifuge (Shanghai Anting Scientific Instrument Factory), RE-52AA rotary evaporator (Shanghai Jiapeng Technology Co., Ltd.), KQ-100B ultrasonic cleaner (Kunshan Ultrasound Instrument Co., Ltd.), 766-2 Far Infrared Fast Drying Box (Huyue Scientific Instrument Factory), SZ-93 Automatic Double Pure Water Distiller (Shanghai Yarong Biochemical Instrument Factory), WQF-200 Fourier Transform Infrared Spectrometer (Beijing Second Optical Instrument Factory).

[0022] 2. Reagents:

[0023] Pyridine, chlorosulfonic acid, absolute ethanol, 2.5mol·L -1 NaOH (the above reagents are analytically pure)

[0024] 3. Identification of Polyporus polysaccharide:

...

example 2

[0034]Place the three-necked flask with a condenser tube and a stirring device in a brine-ice bath, add 40ml of pyridine, stir vigorously to make it fully cooled, slowly add 5ml of chlorosulfonic acid with a dropping funnel, and drop it in 30min. A large number of light yellow solids appeared in the solution, and the esterification reagent was obtained; 0.5 g of polyporus polysaccharide powder was suspended in 40 ml of anhydrous formamide to obtain a polyporus polysaccharide suspension; slowly, the polyporus polysaccharide suspension was added to the esterification reagent, and quickly Move the three-necked bottle into a boiling water bath (100°C), stir at a constant temperature for 1.8-2.2 hours, cool to room temperature, pour the reaction solution into ice water 0.3-0.5 times the reaction solution, and neutralize it with 2.5mol / L NaOH to The pH value is 6-8; after neutralization, add absolute ethanol so that the volume concentration of absolute ethanol is 75-80%, stir and pre...

example 3

[0036] Put the three-necked flask with a condenser tube and a stirring device in an ice-salt bath, add 60ml of pyridine, stir vigorously to make it fully cooled, slowly add 15ml of chlorosulfonic acid with a dropping funnel, and drop it in 40min. Appear a large amount of light yellow solid, obtain esterification reagent; suspend 2.0g polyporus polysaccharide powder in anhydrous formamide 40ml, obtain polyporus polysaccharide suspension; slowly add polyporus polysaccharide suspension in the esterification reagent, rapidly Move the three-necked bottle into a boiling water bath (100°C), stir at constant temperature for 2.8-3.2 hours, cool to room temperature, pour the reaction solution into ice water 0.3-0.5 times the reaction solution, and neutralize it to pH with 2.5mol / L NaOH The value is 6-8; after neutralization, add absolute ethanol to make the volume concentration of absolute ethanol 75-80%, stir and precipitate, centrifuge the liquid and solid, collect the precipitate A; d...

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Abstract

The present invention relates to a preparation method of polyporus polysaccharide sulfate. Said method includes the following steps: (1). preparing esterification reagent; (2). selecting and using polyporus polysaccharide powder and anhydrous methanamide or dimethyl methanamide according to mass ratio of 0.4-2.0:36-48, making the polyporus polysaccharide powder be suspended in anhydrous methanamide or dimethyl methanamide to obtain polyporus polysaccharide suspension; (3). making esterification reaction; (4). precipitation; (5). purifications and (6). secondary precipitation. Besides, said invention also provides the concrete requirement of every step.

Description

technical field [0001] The invention relates to a preparation method of polysaccharide sulfate mainly used for anti-hepatitis B virus (HBV) activity, and specifically relates to the active ingredient in Polyporus polysaccharides, which is water-soluble Polyporus polysaccharide, is sulfated and modified to prepare pig The method of Ling polysaccharide sulfate ester. Background technique [0002] Hepatitis B is a serious public health problem. It has the characteristics of strong infectivity, wide prevalence and high incidence. This disease is the most harmful one among the seven known hepatitis. This kind of infection can eventually develop into liver cirrhosis and liver cancer, which is the ninth cause of death among human diseases announced by the WTO. At present, there are more than 300 million people infected with chronic hepatitis B virus in the world, and according to a rough estimate in my country in the past two years, about 130 million people are infected with chron...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P1/16A61P31/12
Inventor 刘小平徐述明王莹万水昌
Owner WUHAN UNIV OF TECH
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