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Application of water-soluble multi-wall carbon nanotube as substrate in MALDI-MS

A multi-walled carbon nanotube, water-soluble technology, applied in the direction of nanotechnology, nanotechnology, nanostructure manufacturing, etc., can solve problems such as difficult to accurately control the amount of matrix added, low desorption ionization efficiency, and weak surface interaction of metal targets , to achieve the effects of convenient quantitative analysis, improved desorption ionization efficiency, and convenient sample pointing operation

Inactive Publication Date: 2008-11-05
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the strong hydrophobicity of multi-walled carbon nanotubes (almost insoluble in water and general organic solvents), it is used as a MALDI matrix: (1) it is difficult to accurately control the amount of matrix added, and the repeatability of the operation is poor (2) It is difficult to form a uniform matrix layer, the repeatability of the detection signal is poor, and it is difficult to carry out quantitative analysis; (3) The matrix layer is loose and has weak interaction with the metal target surface, and it is easy to fly away from the target surface in the vacuum electric field of the ion source. Contaminated ion source
In addition, because the crude product of multi-walled carbon nanotubes contains catalysts, amorphous carbon, graphite fragments and other impurities, it is not efficient to desorb and ionize analytes as a MALDI matrix.

Method used

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  • Application of water-soluble multi-wall carbon nanotube as substrate in MALDI-MS
  • Application of water-soluble multi-wall carbon nanotube as substrate in MALDI-MS
  • Application of water-soluble multi-wall carbon nanotube as substrate in MALDI-MS

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Water-soluble multi-walled carbon nanotubes used as MALDI-MS matrix for mass spectrometric analysis of Chinese medicine Coptidis Rhizoma extract

[0031] Preparation of water-soluble multi-walled carbon nanotubes:

[0032] (1) Weigh 200 mg of multi-walled carbon nanotubes and place them in a 100 mL flask, and slowly add 10 mL of concentrated nitric acid and 30 mL of concentrated sulfuric acid successively;

[0033] (2) Electromagnetic stirring, heated to 120°C and refluxed for 30min;

[0034] (3) remove heating, stir and cool to room temperature;

[0035] (4) Transfer the solution in the flask to a beaker filled with 300mL of cold water, stir and cool to room temperature;

[0036] (5) Suction filter the diluted solution with a 0.45 μm organic membrane, and rinse with secondary water until the pH is close to neutral;

[0037] (6) Transfer the filter membrane and the filter cake on the membrane to a beaker filled with 50 mL of absolute ethanol, and take out t...

Embodiment 2

[0045] Example 2 Quantitative analysis of water-soluble multi-walled carbon nanotubes as a MALDI-MS matrix for the components palmatine and jatrorrhizine in the Chinese medicine Coptidis Rhizoma extract

[0046] The specific process is as follows:

[0047] 1) The preparation of the multi-walled carbon nanotube matrix solution is the same as in Example 1;

[0048] 2) The preparation of Coptidis Rhizoma extract is the same as in Example 1;

[0049] 3) Dilute the extract of Coptidis rhizome 1,000,000 times with water, and add 0ng, 5ng, 10ng, 15ng and 20ng of palmatine and jatrorrhizine to 1mL solution respectively;

[0050] 4) Take 1 μL of sample solutions of various concentrations and spot them on the substrate layer, and after natural drying, send them to MALDI-MS instrument for mass spectrometry analysis;

[0051] 5) With berberine as the internal standard, calculate the relative peak areas of palmatine and jatrorrhizine to the internal standard respectively, and calculate t...

Embodiment 3

[0054] Embodiment 3 Water-soluble multi-walled carbon nanotubes and general multi-walled carbon nanotubes water-soluble comparison

[0055] Such as figure 1 As shown, the specific process is as follows:

[0056] (1) Weigh 10 mg of water-soluble multi-walled carbon nanotubes and common multi-walled carbon nanotubes respectively, add 5 mL of water respectively, and let stand for 48 hours after ultrasonication for 3 minutes;

[0057] (2) The water solubility of general multi-walled carbon nanotubes is as follows: figure 1 As shown in figure a on the left in the middle;

[0058] (3) Get the water-soluble multi-walled carbon nanotube solution, and centrifuge (5000rpm, 10min) to remove the undissolved part;

[0059] (4) The water solubility of water-soluble multi-walled carbon nanotubes is as follows: figure 1 As shown in Figure b on the right, the measured solubility is about 1.0 mg / mL.

[0060] The preparation of water-soluble multi-walled carbon nanotubes described in this e...

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Abstract

The invention uses water-soluble multi-wall carbon nano tube as MALDI matrix for MALDI-MS, overcomes the defect of nano tube self, accelerates analysis for small molecular compound, and realizes quantitative and qualitative analysis fast and accurately.

Description

technical field [0001] The invention relates to a matrix material of MALDI, specifically the application of water-soluble multi-walled carbon nanotubes in MALDI-MS as a matrix of MALDI. Background technique [0002] In 1988, Tanaka and Hillenkamp independently proposed a technology to assist analyte desorption and ionization by absorbing laser energy through a matrix, called Matrix Assisted LaserDesorption / Ionization (MALDI). Combined with time-of-flight mass spectrometry, this technology has become a powerful tool in biotechnology. It has been successfully applied to the detection of biological macromolecules such as proteins, DNA / RNA, and polysaccharides, and won the 2002 Nobel Prize in Chemistry. However, the introduction of the matrix brings a large number of matrix peaks in the low molecular weight range, resulting in serious background interference, thus limiting the application of this method in the analysis of small molecules. In addition, factors such as the choice...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90B82B3/00
Inventor 邹汉法潘晨松徐松云苏兴业张宇
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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