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Process for preparing immobilization compound microorganism preparation for controlling secondary pollution of river channel bed mud

A compound bacterial agent, secondary pollution technology, applied in microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of frequent dosing, sediment resuspension, high cost, and achieve pollution release. The effect of falling, not easy to run off, and reducing pollution

Inactive Publication Date: 2009-07-29
GUANGZHOU INST OF GEOCHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, the measures used to control the secondary pollution of sediment were mainly dredging and dredging. This method is costly, and the reprocessing of sediment is also a complex environmental protection problem; through aeration and reoxygenation of water body, sediment pollution can be reduced to a certain extent , but it is easy to cause sediment re-suspension in shallow rivers; adding microbial preparations in rivers, this method has a certain effect on enhancing the self-purification ability of water bodies, but the disadvantage is that microbial preparations are easy to be lost in rivers, frequent dosing, and the cost is too high high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Firstly, the immobilized composite bacterial agent was prepared, and then the application comparison experiment was carried out in the simulated river channel.

[0020] The preparation method of immobilized composite bacterial agent is as follows:

[0021] Using Rhodopseudomonas palustris (Rhodopseudomonas palustris) ZZH-01 strain as the bacterial strain, the bacterial strain was inserted into the liquid medium, and cultured under anoxic conditions with an illumination of 1000 lx, a temperature of 30°C, and a cultivation time of 72 hours to obtain seed culture 10% of the inoculum size was added to the liquid medium for amplified culture, under anoxic conditions, the light intensity was 1000 lx, the temperature was 30°C, the culture time was 96 hours, and the bacterial content was 20×10 8 photosynthetic bacteria fermentation broth per milliliter. The formula of photosynthetic bacteria liquid culture medium is calculated by mass fraction: ammonium chloride (NH 4 Cl) 0.1...

Embodiment 2

[0026] Use the Rhodopseudomonas palustris ZZH-02 strain as the bacterial strain, insert the bacterial strain into the liquid medium, and cultivate under anoxic conditions with an illumination of 2000 lx, a temperature of 25°C, and a cultivation time of 48 hours to obtain seed culture liquid; then according to the inoculum amount of 5% by mass fraction, it was inserted into the liquid medium for amplified culture, under anoxic conditions, the light intensity was 1000 lx, the temperature was 25°C, and the culture time was 72 hours, and the bacterial content was 10×10 8 photosynthetic bacteria fermentation broth per milliliter. The formula of photosynthetic bacteria liquid culture medium is calculated by mass fraction: ammonium chloride (NH 4 Cl) 0.10%, dipotassium hydrogen phosphate (K 2 HPO 4 )0.02%, sodium acetate (CH 3 COONa) 0.40%, magnesium sulfate (MgSO 4 ·7H 2 O) 0.05%, sodium chloride (NaCl) 0.40%, calcium chloride (CaCl 2 ): 0.02%, yeast extract 0.02%, water 98.99...

Embodiment 3

[0031] Using Rhodopseudomonas palustris ZZH-03 strain as the bacterial strain, the bacterial strain was inserted into the liquid medium, and cultured under anoxic conditions with a light intensity of 3000 lx, a temperature of 35°C, and a culture time of 72 hours to obtain seed culture liquid; then 15% of the inoculum size was inserted into the liquid medium for amplified culture, under anoxic conditions, the light intensity was 3000 lx, and the temperature was 35 ° C. The culture time was 96 hours, and the bacterial content was 20 × 10 8 photosynthetic bacteria fermentation broth per milliliter. The formula of photosynthetic bacteria liquid culture medium is calculated by mass fraction: ammonium chloride (NH 4 Cl) 0.12%, dipotassium hydrogen phosphate (K 2 HPO 4 )0.03%, sodium acetate (CH 3 COONa) 0.5%, magnesium sulfate (MgSO 4 ·7H 2 O) 0.07%, sodium chloride (NaCl) 0.5%, calcium chloride (CaCl 2 ): 0.04%, yeast extract 0.04%, water 98.7%.

[0032] Use Bacillus subtili...

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PUM

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Abstract

The invention relates to a method for preparing an immobilized composite microbial agent for controlling secondary pollution of river bottom mud, which is characterized in that it comprises preparing photosynthetic bacteria fermentation culture liquid and Bacillus fermentation culture liquid respectively, and preparing immobilized Chemical compound bacteria agent. The immobilized composite bacterial agent of the present invention is not easy to be lost after being put into the polluted river course, and not only exerts the degradation effect of the microbial bacterial agent on pollutants, but also exerts the adsorption and fixation effect of zeolite powder on the pollutants released from the bottom mud, so as to reduce the impact of the bottom mud on the upper The purpose of polluting the covered water body. Tests have shown that the use of the immobilized composite bacterial agent developed in the present invention can significantly reduce the release of pollution from the sediment to the overlying water body.

Description

technical field [0001] The invention relates to a preparation method of an immobilized composite bacterial agent for controlling secondary pollution of river bottom mud. Background technique [0002] Due to the long-term settlement and accumulation of domestic pollutants, the black and smelly bottom mud of urban rivers is silted up. Urban river bottom mud pollution is an important source of black and smelly water bodies. Especially when external source pollution of water is controlled, bottom mud pollution becomes the source of water body pollution. main source. The river sediment contains a large amount of organic substances and nutrients, and has a high oxygen consumption rate. Generally, it is in a strong reducing environment. Secondary pollution of the sediment can easily lead to high levels of harmful substances such as ammonia nitrogen, nitrite, sulfide, and skatole in the water body. It causes "black and smelly" water bodies, affecting urban landscape and residents' ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/38C12R1/125C02F3/34
Inventor 陈繁忠徐文新盛彦清傅家谟
Owner GUANGZHOU INST OF GEOCHEMISTRY - CHINESE ACAD OF SCI
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