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An auto T cell vaccine against T lymphocytoma and preparation method thereof

A lymphocyte and cell technology, applied in the field of vaccines, can solve the problem of down-regulation of T cell expression

Inactive Publication Date: 2009-12-09
SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, activated T cells in tumor patients downregulate

Method used

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  • An auto T cell vaccine against T lymphocytoma and preparation method thereof
  • An auto T cell vaccine against T lymphocytoma and preparation method thereof
  • An auto T cell vaccine against T lymphocytoma and preparation method thereof

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Experimental program
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Embodiment Construction

[0020] 1. Materials and methods:

[0021] Mice: Female C57 / B1 / 6 mice were purchased from Shanghai Experimental Animal Center, Chinese Academy of Sciences. Raised in a sterile environment and used at the age of 4 to 8 weeks;

[0022] Prepare autologous activated T cells in complete essential medium DMEM: 10% calf serum, 10mM HEPES, 50uM β-mercaptoethanol, 2mML-glutamine, 50IU / ml penicillin and streptomycin; make isolated and purified splenocytes (3×10 6 / ml) were stimulated with 2ug / ml ConA (VectorLaboratories, Burlingama, CA) for 72 hours. Cells were collected and washed three times with PBS (pH7.4). T cells were then purified by negative selection using specific antibody-coated magnetic beads (Dynal Biotech, Oslo, Norway). The resulting T cell purity was greater than 92%. Purified T cells were irradiated with 3000 rad by an illumination instrument and used as autologous activated T cells for subsequent immunization.

[0023] Optimal experimental conditions for immunizatio...

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Abstract

The invention relates the anti T-lymphoblast vaccine and preparing method. The anti T-lymphoblast vaccine uses the T cell as vaccine. The method comprises the following steps: putting the T cell in the culture medium DMEM, which contain 10% cattle serum, 10mMHEPES, 50uM beta mercaptoethanol, 2mML glutamine, 50IU / ml penicillin; separating them, getting 3X106 / ml splenocyte, stimulating it with 2ug / mlConA for 72 hours, collecting cells, washing three times with PBS whose pH is 7.4; purifying T cell with magnetic bead coated by specificity antibody, irradiating the T cell with 3000rad illumination, and getting the activatable T cell. The application area of the vaccine comprises the malignant tumor, including T lymph hump.

Description

Technical field [0001] The invention relates to a vaccine, in particular to a self-T cell vaccine capable of improving anti-T lymphoma and other types of tumors, its preparation method and application. Background technique [0002] Autoreactive T cells are involved in the occurrence of autoimmune diseases, inoculation of unactivated autoreactive T cells can cause anti-idiotypic responses, thereby reducing the autoreactive T cells that cause autoimmune diseases. However, whether inoculation with attenuated healthy autologous T cells can increase the antitumor response is not clear. [0003] Surveillance functions of the immune system are known to suppress tumor growth in vivo. But in many cases, tumors can escape the immune system's surveillance by limiting an effective immune response. Although some tumor antigens on the surface of tumor cells are believed to mediate the anti-tumor immune response to temporarily disappear tumor cells, most of the malignant tumor cells in t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/00A61K35/26C12N5/08A61P35/00A61K35/17C12N5/0783
Inventor 李宁丽沈佰华王利
Owner SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE