Method for detecting mononucleotide polymorphism with biochip

A single nucleotide polymorphism, biochip technology, applied in biochemical equipment and methods, microbial determination/inspection, fluorescence/phosphorescence, etc., can solve the problems of high detection cost and difficulty in achieving high-throughput detection. Simplified operation steps, significant advantages, low non-specific binding rate

Active Publication Date: 2010-02-10
SHANXI LIFEGEN
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  • Abstract
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Problems solved by technology

[0013] The purpose of the present invention is to provide a method for detecting single nucleotide polymorphisms on a biochip, which solves the technical problems of difficult to achieve high-throughput detection and high detection cost in the background technology

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  • Method for detecting mononucleotide polymorphism with biochip
  • Method for detecting mononucleotide polymorphism with biochip

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Embodiment Construction

[0073] Nucleic acid amplification technology is a rapid and economical technique for synthesizing a large number of copies of DNA fragments. Commonly used in prenatal diagnosis of genetic diseases, detection and diagnosis of cancer genes, detection of pathogenic pathogens, DNA fingerprinting, individual identification, parent-child relationship identification and forensic evidence, animal and plant quarantine, rapid diagnostic testing, gene splicing, sequencing and other molecules field of biology.

[0074] The ligase reaction is a technique for linking two nucleic acid fragments, which is often used in the field of molecular biology such as molecular cloning.

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Abstract

This invention relates to a method of biochip detecting mononucleotide polymorphism. first amplify nucleic acid sample, obtain amplifying product; then take solution hybridization and joining enzyme reaction, connect complete matching probe in solution hybridization with currency probe, to detect mononucleotide polymorphism; then carry out solid-phase hybridization, make label probe with 5' end band, probe 3's end with detecting base group and biochip to carry out hybridization, temperature 25 - 75deg, time 0.5h to 36h, detecting base group be distributed to different position of biochip; Carry out mononucleotide polymorphism detecting, gain detecting outcome. This invention solves technical problems of background technology hard of realizing high flux reactor detecting and high detectingcost.

Description

technical field [0001] The invention belongs to the field related to gene detection, is applicable to the nucleotide identification of a specific site on a nucleic acid molecule, and specifically relates to a method for detecting single nucleotide polymorphism with a biochip. Background technique [0002] In 2001, The SNP Consortium constructed a 1.42×10 6 A human genetic map with a single SNP and a density of one single nucleotide polymorphism site per 1.9kb fragment, which is very important for the study of disease-causing genes and human origin and evolution. It is estimated that about 100,000 SNP molecular markers will be used for gene function and disease-related research. Such a huge analysis requirement places extremely high requirements on detection technology. [0003] At present, the common detection methods for SNPs of alleles mainly include the following: [0004] The allele-specific amplification method is to make the 3′ end of the designed primer 1 complemen...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64C12Q1/68
Inventor 陈超聂萌杜蓬李铮
Owner SHANXI LIFEGEN
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