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Method for forming film, base plate with film, sensor, and liquid composition

A composition and liquid technology, applied in chemical method analysis, instruments, scientific instruments, etc., can solve the problems of protein physiological activity reduction and inactivation

Inactive Publication Date: 2007-08-29
SEIKO EPSON CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, if such an ink is used to immobilize a protein on a substrate, the polyether compound contained in the ink comes into contact with the protein, thereby causing a problem that the physiological activity of the protein is reduced (deactivated).

Method used

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  • Method for forming film, base plate with film, sensor, and liquid composition
  • Method for forming film, base plate with film, sensor, and liquid composition
  • Method for forming film, base plate with film, sensor, and liquid composition

Examples

Experimental program
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Effect test

Embodiment 1

[0203] First, a glass substrate was prepared, and an Ag electrode (working electrode) having an average thickness of 200 nm was formed by vacuum deposition.

[0204] Next, albumin, which is a bio-related polymer, was dissolved in pure water to prepare an albumin-containing solution.

[0205] Next, the albumin-containing solution was supplied onto the working electrode by an inkjet method to form a liquid coating, and then dried under the conditions of 60° C.×30 minutes in an atmospheric pressure atmosphere to obtain a base layer.

[0206] Next, the liquid composition of sample No. 1 is supplied to the ink chamber of the inkjet printer (manufactured by Seiko Epson Corporation, "PM700"), and then the piezoelectric element provided in the ink chamber is operated to generate The form of droplets is provided onto the substrate layer. In this way, a liquid film is formed on the base layer. In addition, the viscosity and surface tension of the liquid composition were 3.6 cP and ...

Embodiment 2

[0212] In said process , the sensor was produced similarly to Example 1 except having used sample No. 2 instead of sample No. 1 as a liquid composition.

Embodiment 3

[0214] In said process , the sensor was produced similarly to Example 1 except having used sample No. 3 instead of sample No. 1 as a liquid composition.

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Abstract

The film forming method of the invention is a method for forming a detection film including the detection substance for detecting an object on one side of a base plate. The method is characterized in that it includes: a first step for preparing a liquid composition containing a substance for detecting an object and a polyether derivative having a weight-average molecular weight of 2000 or less; a second step for supplying the liquid composition to the said side by using a droplet discharge method to form a liquid film; and a third step for drying the liquid film to form the detection film. Therefore, the invention provides a film forming method which can form a film including protein with excellent film forming accuracy, and can restrain or prevent the reduction of the physiological active of the protein in the film, a base plate with the film formed by using the film forming method and a sensor with high reliability. The invention also provides a liquid composition which can prevent or restrain the reduction of the physiological active of the protein in the film and can form a film including protein with excellent film forming accuracy.

Description

technical field [0001] The present invention relates to a film forming method, a substrate with a film, a sensor, a liquid composition and the like. Background technique [0002] In recent years, for example, proteins having physiological activities such as enzymes and antibodies can be immobilized on substrates, and the biological reactions in which these proteins participate can be observed (detected) in vitro in real time. [0003] In order to observe this biological reaction with higher precision, it is necessary to support the protein on the substrate with excellent film-forming accuracy, but as a method of immobilizing the protein on the substrate, it is widely used to use a protein-containing liquid as an ink and place the protein on the substrate. An inkjet method in which ink is supplied onto a substrate (for example, refer to Patent Document 1.). [0004] In the inkjet method, the vibrating plate vibrated by the piezoelectric element is vibrated, the volume of the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/50G01N31/00
Inventor 福岛均泷口宏志
Owner SEIKO EPSON CORP
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