Desmodium styracifolium extract and preparation method and application thereof
A technology of Desmodium sativa and extract, which is applied in the field of extracts and preparations of Desmodium sativa, can solve problems such as no cigarette application reports, and achieve the effects of easy separation and purification, no environmental pollution, and quality improvement
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Embodiment 1
[0021] Weigh a certain amount of Desmodium glabra powder passing through a 40-mesh sieve, add 75% ethanol solution according to the ratio of 1:15, mix and place in a high-pressure container, keep the pressure and extract for 20min, the extraction pressure is 300Mpa, filter after pressure relief Remove the filter residue, adjust the pH of the filtrate to 7.5 to 8.0 with 20% sodium hydroxide solution, refrigerate at 0°C to 4°C for 10 hours, and centrifuge at a centrifugal rate of 3000rpm for 20 minutes to obtain a supernatant. When concentrated under reduced pressure to 25°C, the relative contrast is 1.2, which is Desmodium sativa extract I. After diluting it 10 times with propylene glycol, add it to shredded tobacco according to 0.2% of the weight of shredded tobacco, and then make finished tobacco according to the existing technology.
Embodiment 2
[0023] Weigh a certain amount of Desmodium glabra powder passing through a 40-mesh sieve, add 95% ethanol solution according to the ratio of 1:16, mix and place in a high-pressure container, keep the pressure and extract for 25min, the extraction pressure is 500Mpa, filter after pressure relief Remove the filter residue, adjust the pH of the filtrate to 7.5 to 8.0 with 20% sodium hydroxide solution, put it in the refrigerator at 0°C to 4°C for 8 hours, and centrifuge at a centrifugal speed of 5000rpm for 15 minutes to obtain the supernatant. When concentrated under reduced pressure to 25°C, the relative contrast is 1.2, which is Desmodium glabra extract II. After diluting 10 times with sorbitol, it is added to shredded tobacco according to 0.1% of the weight of shredded tobacco, and then made into finished tobacco according to the existing technology.
Embodiment 3
[0025] Weigh a certain amount of Desmodium glabra powder through a 40-mesh sieve, add 80% ethanol solution according to the ratio of 1:12, mix and place in a high-pressure container, keep the pressure and extract for 30min, the extraction pressure is 600Mpa, filter after pressure relief Remove the filter residue, adjust the pH of the filtrate to 7.5-8.0 with 20% sodium hydroxide solution, place it in the refrigerator at 0°C-4°C for 16 hours, and then centrifuge at 8000rpm for 10 minutes to obtain the supernatant. When concentrated under reduced pressure to 25°C, the relative contrast is 1.2, which is Desmodium sativa extract III. After diluting it 10 times with propylene glycol, it is added to shredded tobacco at 0.5% of the weight of shredded tobacco, and then made into finished tobacco according to the existing technology.
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