Silk fibroin and polymeric lactic acid compound film and its preparation method

A technology of polylactic acid and composite membrane, which is applied in the direction of non-active components of polymer compounds, medical science, and absorbent pads, etc., can solve the problems of easy water loss, high dissolution rate of silk fibroin membrane, and poor mechanical properties, etc., to achieve convenient Storage and transportation, good vapor and moisture permeability, and good biocompatibility

Inactive Publication Date: 2011-02-02
ZHEJIANG SCI-TECH UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the preparation of silk fibroin membrane, the patents that have been reported include Li Mingzhong of Soochow University and others who have developed a porous silk fibroin membrane, the patent number is: ZL 00102103.6, which uses pure silk fibroin solution to obtain the pore size distribution by freeze-drying method. The porous silk film with a thickness of 1-500 μm can be used as a scaffold material for tissue engineering. Due to its large pore size, easy loss of water, and poor mechanical properties, it is not suitable for use in wound masks.
In addition, they also developed a flexible silk film, the patent number is: ZL 01134165.3, in this patent, epoxy resin is added to the silk fibroin aqueous solution as a cross-linking agent, and the film is formed by a drying method to obtain a film with better flexibility. , but because the film contains epoxy resin with poor biocompatibility, its application in medicine is limited
The patent No. is: ZL01108134.1, which discloses an antibacterial silk fibroin wound mask and its preparation method. It adopts the method of adding antibacterial agents and additives into the silk fibroin solution to obtain a non-toxic silk fibroin film with antibacterial efficacy, but The film is basically composed of pure silk fibroin, without insolubilization treatment, the obtained silk film has a high dissolution rate and poor mechanical properties. If it is insolubilized with ethanol, the obtained silk film The film is brittle and has little practical application value

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Put 1 kg of silk into 15L of neutral soap solution with a concentration of 2.5%, then move it into a water bath, heat it to 98°C, keep it for 50min, and use deionized water and Na with a concentration of 0.05% respectively 2 CO 3 solution for cleaning.

[0021] 2. Repeat step 1, take a large beaker with a volume of 5L, put the treated silk into it, and add 2L of 0.05% Na 2 CO 3 solution, and then moved into a water bath at 50°C for 20 minutes for deep degumming to obtain refined silk fibroin.

[0022] 3. Use 10L of CaCl with a molar ratio of 1:2:8 2 / C 2 h 5 OH / H 2 The above refined silk fibroin was dissolved in O solution, and the temperature was adjusted to 75° C. for 2 hours during the dissolution.

[0023] 4. Add the dissolved silk fibroin to the cellulose dialysis bag, add deionized water for dialysis for 3 days, and change the water every 12 hours to obtain a silk fibroin solution. Filter the silk fibroin solution with multiple layers of gauze to remove...

Embodiment 2

[0030] 1. Put 1 kg of silk into 15L of neutral soap solution with a concentration of 2.5%, then move it into a water bath, heat it to 98°C, keep it for 50min, and use deionized water and Na with a concentration of 0.05% respectively 2 CO 3 solution for cleaning.

[0031] 2. Repeat step 1, take a large beaker with a volume of 5L, put the treated silk into it, and add 2L of 0.05% Na 2 CO 3 solution, and then moved into a water bath at 50°C for 20 minutes for deep degumming to obtain refined silk fibroin.

[0032] 3. Use 10L of CaCl with a molar ratio of 1:2:8 2 / C 2 h 5 OH / H 2 The above refined silk fibroin was dissolved in O solution, and the temperature was adjusted to 75° C. for 2 hours during the dissolution.

[0033] 4. Add the dissolved silk fibroin to the cellulose dialysis bag, add deionized water for dialysis for 3 days, and change the water every 12 hours to obtain a silk fibroin solution. Filter the silk fibroin solution with multiple layers of gauze to remove...

Embodiment 3

[0040] 1. Put 0.5 kg of silk into 7.5L of neutral soap solution with a concentration of 2.5%, then move it into a water bath, heat it to 98°C, keep it for 50min, and use deionized water and Na with a concentration of 0.05% respectively 2 CO 3 The solution is rinsed clean.

[0041] 2. Repeat step 1, take a large beaker with a volume of 5L, put the treated silk into it, and add 2L of 0.05% Na 2 CO 3 solution, and then moved into a water bath at 50°C for 20 minutes for deep degumming to obtain refined silk fibroin.

[0042] 3. Use 5L of CaCl with a molar ratio of 1:2:8 2 / C 2 h 5 OH / H 2 The above refined silk fibroin was dissolved in O solution, and the temperature was adjusted to 75° C. for 2 hours during the dissolution.

[0043] 4. Add the dissolved silk fibroin to the cellulose dialysis bag, add deionized water for dialysis for 3 days, and change the water every 12 hours to obtain a silk fibroin solution. Filter the silk fibroin solution with multiple layers of gauze ...

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Abstract

The invention relates to a silk and polylactic acid composite membrane and preparation method thereof. Dissolving refining silk in tertiary solution of calcium chloride / ethanol / water, dialyzing three days by deionized water to obtain silk solution, dissolving polylactic acid in dimethyl sulphoxide (DMSO) to get polylactic acid solution, blending the silk solution and the polylactic acid solution in a certain ratio, stiring uniformly, then pouring in a culture dish, drying in an environmental chamber with 40-60 DEG C humidity and 40-80% of relative humidity to get silk / polylactic acid composite membrane. The vapor permeability of the composite membrane gets to 14.4-21.5 g / m2.h, moidture permeability gets to 94.7-113.2 g / m2.h, good mechanical properties. The composite membrane of this invention is capable of using widely in wound protective film, artificial skin, anticoagulation blood material, medicine control material.

Description

technical field [0001] The invention relates to a composite film of silk fibroin and polylactic acid and a preparation method thereof. Background technique [0002] In the existing technology, although various synthetic and natural polymer films can be prepared, such as polytetrafluoroethylene film, polyvinyl alcohol film, polyurethane film, cellulose film, chitosan film, sodium alginate film, etc. , but in the application of medicine, it is not easy to find a film that not only has good biocompatibility, but also has excellent mechanical properties and good vapor and moisture permeability. [0003] Silk is composed of silk fibroin and sericin. Silk fibroin, also known as silk fibroin, is the main part of silk, accounting for about 75% of the total weight. It is a natural polymer compound. Silk fibroin is mainly composed of 18 kinds of amino acids such as tyrosine, alanine and serine. It is a natural protein with good biocompatibility, low toxicity and degradability. Broad...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L15/32A61L15/26A61L27/60A61L27/26A61K47/42A61K47/34
Inventor 陈建勇张加忠冯新星吴君南郑桂芬王永军
Owner ZHEJIANG SCI-TECH UNIV
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