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Cell growth and configuration change checking device based on oxidation/reduction probe, and the checking method

A morphological change and detection device technology, which is applied to measurement devices, biochemical equipment and methods, and microbial determination/inspection.

Inactive Publication Date: 2013-10-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It first emits a beam of light to the solution. Since the passage of light by ordinary cells is blocked and reflected, the growth and morphological changes of cells in the solution can be measured by measuring the transmitted and reflected light. However, optical methods are used in some colored Not very convenient to use in solution

Method used

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  • Cell growth and configuration change checking device based on oxidation/reduction probe, and the checking method
  • Cell growth and configuration change checking device based on oxidation/reduction probe, and the checking method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Detection of cell growth in solution

[0019] Reference attached figure 1 . The present invention includes a reference electrode 1, a generating electrode 2, a collecting electrode 3, and a counter electrode 4. The distance between the generating electrode and the collecting electrode is less than or equal to 100 microns and greater than or equal to X microns. Attached figure 2 This is a schematic diagram of the implementation of the present invention in the form of an interdigital electrode. In the figure, the reference electrode and counter electrode are marked with figure 1 Similarly, the reference number of the generating electrode is 5, and the reference number of the collecting electrode is 6. The present invention is also provided with a two-electrode galvanostatic potentiometer 7 for detecting electrode kinetic parameters on the collecting electrode.

[0020] Here, the function of the reference electrode is mainly to provide a voltage reference, and the ...

Embodiment 2

[0023] Example 2 Detection of cell growth in solution

[0024] The detection device provided in this embodiment is the same as the first embodiment. In the actual test, first immerse the device in the test solution containing lung fibroblasts (V79), and add [Fe(CN) 6 ] 3- / 4- Make the final [Fe(CN) 6 ] 3- / 4- The concentration is 10mM. Taking the reference electrode as a reference, a 0.4V voltage is applied to the generating electrode, and a -0.2V DC voltage is applied to the collecting electrode to superimpose a small AC voltage. Generally, the AC voltage is less than 10mV. The frequency used in this embodiment is from 1Hz To a 1MHz sinusoidal AC voltage with an amplitude of 10mV, the standard electrochemical impedance method was used to test the electrochemical reaction impedance on the collecting electrode. At the beginning of the experiment, the electrode impedance measured on the collecting electrode was 400 ohms, and after 1 hour, the electrode impedance measured on the coll...

Embodiment 3

[0026] Example 3 Measurement of cell morphology changes in solution

[0027] The structure of the detection device of this embodiment is the same as that of the first embodiment. In the actual test, first immerse the device in the test solution containing lung fibroblasts (V79), and add [Fe(CN) 6 ] 3- / 4- Make the final [Fe(CN) 6 ] 3- / 4- The concentration is 10mM. Using the reference electrode as a reference, a voltage of 0.4V was applied to the generating electrode and a voltage of -0.2V was applied to the collecting electrode to test the current flowing through the collecting electrode, which was 300 microamperes. Then, mercury chloride was added to the solution, and the concentration of mercury chloride in the final solution was 80 micromolar, and the current flowing through the test collection electrode was 420 microamperes. Comparing the above current values, we can infer the cell growth and morphological changes in the tested solution, and obtain the results of the effect o...

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Abstract

The invention provides a device for checking the cell growth and shape change, based on oxidization / reduction probe, comprising a reference electrode, a generating electrode, a collecting electrode, and an opposite electrode. The distance between the generating electrode and the collecting electrode is not larger than 100mum. And the invention also comprises a dual-electrode constant-current potential machine for checking the electrode dynamic parameter of the collecting electrode. The invention also provides a cell growth check method of the device, which comprises that interval measuring the current or electrochemical reaction resistance on the collecting electrode, comparing the data to obtain the result. The invention also provides a cell shape change check method of the device. The invention measures and compares the current or electrochemical reaction resistances on the collecting electrode, after and before adding the agent which can affect the cell growth and shape change, to obtain the agent effect on the cell growth and shape change. The invention has higher sensitivity on the check device and check method.

Description

Technical field [0001] The invention relates to a cell growth and morphological change detection device and method based on oxidation / reduction probes. The main application fields of the present invention include biochemistry, environmental protection, food safety, etc. Background technique [0002] The growth and morphological changes of cells are a parameter that is very important in many fields. For example, in the fermentation process, the number of cells in the solution is an important indicator of the fermentation process. In the development of modern new drugs, drug screening is often achieved through the influence of various compounds on cell growth, morphology, and performance, such as the adhesion of cells and container walls. In environmental testing, the impact of river water, lake water and other aqueous solutions on cell growth is also a common method of environmental monitoring. [0003] At present, the main detection method for cell growth and morphological change...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/416C12Q1/02
Inventor 吴坚
Owner ZHEJIANG UNIV