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Antibody/ antigen or DNA hybridization detection device based on oxidation/reduction probe, and the checking method

A detection device and antibody technology, which is applied in the direction of measuring devices, biochemical equipment and methods, and microbial measurement/inspection, can solve problems such as increased complexity, and achieve the effect of large parameter variation range and high detection sensitivity

Inactive Publication Date: 2007-10-24
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most immunosensors also require an additional labeled antibody to convert the combination of antigen and antibody into a detectable signal, which increases the complexity of detection, so it is necessary to develop a label-free immunodetection sensor

Method used

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  • Antibody/ antigen or DNA hybridization detection device based on oxidation/reduction probe, and the checking method
  • Antibody/ antigen or DNA hybridization detection device based on oxidation/reduction probe, and the checking method
  • Antibody/ antigen or DNA hybridization detection device based on oxidation/reduction probe, and the checking method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The detection of embodiment 1 escherichia coli

[0016] Refer to accompanying drawings 1 and 2. The present invention includes a reference electrode 1, a generating electrode 2, a collecting electrode 3 and a counter electrode 4, the distance between the generating electrode and the collecting electrode is less than or equal to 100 microns, and a double electrode constant for detecting the electrode kinetic parameters on the collecting electrode Current Potentiometer8. Accompanying drawing 3 is the implementation schematic diagram that the present invention adopts the interdigitated electrode form, and in the figure, the reference numeral of reference electrode, counter electrode is identical with Fig. 1 and 2, and the reference numeral of producing electrode is 6, and the reference numeral of collecting electrode The number is 7.

[0017] Depending on the detection object, if it is the detection of antigen or pathogenic bacteria, the antibody of the detection object ...

Embodiment 2

[0021] The detection of embodiment 2 escherichia coli

[0022] The detection device provided in this embodiment is the same as that in Embodiment 1. During the detection process, the device was first immersed in a solution containing 10mM [Fe(CN) 6 ] 3- / 4- In the solution, with the reference electrode as the reference, a voltage of 0.4V is applied to the generating electrode, and a DC voltage of -0.2V is applied to the collecting electrode to superimpose an AC voltage with a smaller amplitude. Generally, the AC voltage is less than 10mV. In this embodiment Using a sinusoidal AC voltage with a frequency from 1Hz to 1MHz and an amplitude of 10mV, the standard electrochemical impedance method is used to test the electrochemical reaction impedance on the collecting electrode before and after adding the test solution containing E. coli, and compare to realize the detection of E. coli. Comparing the impedance on the collecting electrode before and after the biological reaction, wh...

Embodiment 3

[0024] The detection of embodiment 3DNA hybridization

[0025] The structure of the detection device in this embodiment is basically the same as that in Embodiment 1, except that single-stranded DNA is immobilized on the electrode surface instead of E. coli antibodies. In the actual test, the device is first immersed in a solution containing 10mM [Fe(CN) 6 ] 3- / 4-In the solution, using the reference electrode as a reference, apply a voltage of 0.4V to the generating electrode, and apply a DC voltage of -0.2V to the collecting electrode, and test the electrochemical reactions on the collecting electrode before and after adding the test solution containing the corresponding DNA single strand. Impedance: compare the impedance on the collecting electrode before and after the reaction to realize the detection of DNA hybridization.

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Abstract

The invention provides a hybridization combination check device of antibody / antigen or DNA based on oxidization / reduction probe, comprising a reference electrode, a generating electrode, a collecting electrode, and an opposite electrode, wherein the distance between the generating electrode and the collecting electrode is not longer than 100micrometer, the surfaces of the generating electrode and the collecting electrode are fixed with the antibody or single strand DNA molecule of the object, and the invention also comprises a dual-electrode constant-current potential meter for checking the electrode dynamic parameter of the collecting electrode. The invention can improve the check sensitivity. And the invention also discloses a relative check method, which measures and compares the current or electrochemical reaction resistances of the collecting electrode before and after the biological reaction to obtain the result. The invention measures the electrochemical reaction parameters on the collecting electrode, with large parameter change range and high sensitivity.

Description

technical field [0001] The invention relates to an antibody / antigen or DNA hybrid detection device and method based on an oxidation / reduction probe. The main application fields of the invention include biochemistry, environmental protection, food safety and the like. Background technique [0002] Immunoassay technology is a rapid detection technology for qualitative or quantitative determination of antigen or antibody by using the specific reaction between antigen and antibody, including enzyme immunoassay technology, fluorescent immunoassay technology, immunocolloidal gold labeling technology, immunomagnetic beads technology etc. However, most immunosensors also require an additional labeled antibody to convert the combination of antigen and antibody into a detectable signal, which increases the complexity of detection, so it is necessary to develop a label-free immunodetection sensor. At present, some immunosensors without labeling have been de...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N27/416C12Q1/68
Inventor 吴坚
Owner ZHEJIANG UNIV